Highly-Sensitive Polymer Optical Fiber SPR Sensor for Fast Immunoassay
Abstract A new type of human immunoglobulin G (IgG) sensors based on the surface plasmon resonance (SPR) in the low refractive index (RI) plastic optical fiber (POF) and an antibody immobilization method is presented. A 50-nm-thick gold film was formed on the polished D-shaped fiber surface by magne...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2024-05-01
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| Series: | Photonic Sensors |
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| Online Access: | https://doi.org/10.1007/s13320-024-0729-x |
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| author | Ying Wang Xing Rao Xun Wu George Y. Chen Changrui Liao Mateusz Jakub Smietana Yiping Wang |
| author_facet | Ying Wang Xing Rao Xun Wu George Y. Chen Changrui Liao Mateusz Jakub Smietana Yiping Wang |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | Abstract A new type of human immunoglobulin G (IgG) sensors based on the surface plasmon resonance (SPR) in the low refractive index (RI) plastic optical fiber (POF) and an antibody immobilization method is presented. A 50-nm-thick gold film was formed on the polished D-shaped fiber surface by magnetron sputtering. The RI response of the POF sensor is 30 049.61 nm/RIU, which is 26.5 times higher than that of single mode fiber (SMF) SPR sensors. The proposed SPR biosensor can be developed by simple and rapid modification of the gold film with 11-mercapto undecanoic acid (MUA). Upon immobilization of the goat anti-human IgG antibody, the resonance wavelength shifts by 11.2 nm. The sensor can be used to specifically detect and quantify the human IgG at concentrations down to 245.4 ng/mL with the sensitivity of 1.327 7 nm per µg/mL, which offers an enhancement of 12.5-fold compared to that of the conventional SMF based SPR sensors. The proposed device may find the potential applications in the case of use at the point of care. |
| format | Article |
| id | doaj-art-3fc41fa803ef42519a018e4a1f7558fb |
| institution | Kabale University |
| issn | 1674-9251 2190-7439 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Photonic Sensors |
| spelling | doaj-art-3fc41fa803ef42519a018e4a1f7558fb2025-02-09T12:26:21ZengSpringerOpenPhotonic Sensors1674-92512190-74392024-05-0114411110.1007/s13320-024-0729-xHighly-Sensitive Polymer Optical Fiber SPR Sensor for Fast ImmunoassayYing Wang0Xing Rao1Xun Wu2George Y. Chen3Changrui Liao4Mateusz Jakub Smietana5Yiping Wang6Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education/Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen UniversityKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education/Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen UniversityKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education/Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen UniversityKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education/Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen UniversityKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education/Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen UniversityDivision of Microsystem & Electronic Materials Technology, Institute of Microelectronics & Optoelectronics, Warsaw University of TechnologyKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education/Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen UniversityAbstract A new type of human immunoglobulin G (IgG) sensors based on the surface plasmon resonance (SPR) in the low refractive index (RI) plastic optical fiber (POF) and an antibody immobilization method is presented. A 50-nm-thick gold film was formed on the polished D-shaped fiber surface by magnetron sputtering. The RI response of the POF sensor is 30 049.61 nm/RIU, which is 26.5 times higher than that of single mode fiber (SMF) SPR sensors. The proposed SPR biosensor can be developed by simple and rapid modification of the gold film with 11-mercapto undecanoic acid (MUA). Upon immobilization of the goat anti-human IgG antibody, the resonance wavelength shifts by 11.2 nm. The sensor can be used to specifically detect and quantify the human IgG at concentrations down to 245.4 ng/mL with the sensitivity of 1.327 7 nm per µg/mL, which offers an enhancement of 12.5-fold compared to that of the conventional SMF based SPR sensors. The proposed device may find the potential applications in the case of use at the point of care.https://doi.org/10.1007/s13320-024-0729-xOptical fiber biosensorsurface plasmon resonanceantibody immobilizationside-polished fiberplastic optical fiber |
| spellingShingle | Ying Wang Xing Rao Xun Wu George Y. Chen Changrui Liao Mateusz Jakub Smietana Yiping Wang Highly-Sensitive Polymer Optical Fiber SPR Sensor for Fast Immunoassay Photonic Sensors Optical fiber biosensor surface plasmon resonance antibody immobilization side-polished fiber plastic optical fiber |
| title | Highly-Sensitive Polymer Optical Fiber SPR Sensor for Fast Immunoassay |
| title_full | Highly-Sensitive Polymer Optical Fiber SPR Sensor for Fast Immunoassay |
| title_fullStr | Highly-Sensitive Polymer Optical Fiber SPR Sensor for Fast Immunoassay |
| title_full_unstemmed | Highly-Sensitive Polymer Optical Fiber SPR Sensor for Fast Immunoassay |
| title_short | Highly-Sensitive Polymer Optical Fiber SPR Sensor for Fast Immunoassay |
| title_sort | highly sensitive polymer optical fiber spr sensor for fast immunoassay |
| topic | Optical fiber biosensor surface plasmon resonance antibody immobilization side-polished fiber plastic optical fiber |
| url | https://doi.org/10.1007/s13320-024-0729-x |
| work_keys_str_mv | AT yingwang highlysensitivepolymeropticalfibersprsensorforfastimmunoassay AT xingrao highlysensitivepolymeropticalfibersprsensorforfastimmunoassay AT xunwu highlysensitivepolymeropticalfibersprsensorforfastimmunoassay AT georgeychen highlysensitivepolymeropticalfibersprsensorforfastimmunoassay AT changruiliao highlysensitivepolymeropticalfibersprsensorforfastimmunoassay AT mateuszjakubsmietana highlysensitivepolymeropticalfibersprsensorforfastimmunoassay AT yipingwang highlysensitivepolymeropticalfibersprsensorforfastimmunoassay |