Oxidative genomic damage in pediatric patients exposed to mercury released by dental amalgam
Abstract Background Mercury (Hg) is the major component of dental amalgam which has been utilized for decades because it is durable, inexpensive and easy to manipulate and position, as well as having a relatively low cost. Hg from dental amalgam fillings has been shown to be released into the buccal...
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BMC
2025-07-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-025-06522-0 |
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| author | Guadalupe Nalleli Macías-Lamas Yveth Marlene Ortiz-García Blanca Patricia Lazalde-Ramos Guillermo Moisés Zúñiga-González Belinda Claudia Gómez-Meda Cristina Hermila Martínez-Bugarín Susana Vanessa Sánchez-De-La-Rosa Saulo Oswaldo Sánchez-Rivera Gabriela Morales-Velazquez Celia Guerrero-Velázquez Ana Lourdes Zamora-Perez |
| author_facet | Guadalupe Nalleli Macías-Lamas Yveth Marlene Ortiz-García Blanca Patricia Lazalde-Ramos Guillermo Moisés Zúñiga-González Belinda Claudia Gómez-Meda Cristina Hermila Martínez-Bugarín Susana Vanessa Sánchez-De-La-Rosa Saulo Oswaldo Sánchez-Rivera Gabriela Morales-Velazquez Celia Guerrero-Velázquez Ana Lourdes Zamora-Perez |
| author_sort | Guadalupe Nalleli Macías-Lamas |
| collection | DOAJ |
| description | Abstract Background Mercury (Hg) is the major component of dental amalgam which has been utilized for decades because it is durable, inexpensive and easy to manipulate and position, as well as having a relatively low cost. Hg from dental amalgam fillings has been shown to be released into the buccal cavity due to processes such as abrasion and corrosion, and which could represent a continuous source of oxidative damage to mouth tissues. Therefore, this study assessed the impact of Hg-containing dental amalgams exposure on the oxidative genomic damage using a means of the buccal micronucleus cytome assay by counting the nuclear abnormalities (NAs) in buccal mucosa cells and by analyzing in whole saliva the molecules 8-hydroxy-2′-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) in whole saliva. Methods This study was conducted on 120 healthy children divided into two groups-group 1 (or the control group) (n = 60), including participants without dental caries, no history of dental amalgam (Hg-containing) restorations, and no other dental treatments, and group 2 (the intervention group) (n = 60), including participants with dental caries involving enamel or dentin, who received dental amalgam restorations in two posterior teeth. Oral epithelial cell and whole saliva samples were taken at baseline and 30 days later for group 1, while for group 2, samples were obtained at different pre-treatment (baseline) and post-amalgam placement time points at 15 and 30 days. Results An increased frequency of NAs (p < 0.05) and higher levels of 8-OHdG (p < 0.05) and MDA (p < 0.05) were observed after Hg-dental amalgams exposure. In addition, a positive correlation existed between NAs and oxidative stress induced by Hg from dental amalgam. Conclusions The presents study shows that individuals exposed to Hg-containing dental amalgam exhibit increased NAs in oral epithelial cells, as well as increased levels of 8-OHdG and MDA in saliva, which are directly related to genotoxicity, oxidative DNA damage and lipid peroxidation, respectively. Exposure to Hg- containing dental amalgams may contribute to oxidative stress and genetic damage, potentially leading to long-term implications for both oral and systemic health. |
| format | Article |
| id | doaj-art-3fc08a69ea254815b133304e148378f9 |
| institution | Kabale University |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-07-01 |
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| series | BMC Oral Health |
| spelling | doaj-art-3fc08a69ea254815b133304e148378f92025-08-20T03:42:03ZengBMCBMC Oral Health1472-68312025-07-0125111210.1186/s12903-025-06522-0Oxidative genomic damage in pediatric patients exposed to mercury released by dental amalgamGuadalupe Nalleli Macías-Lamas0Yveth Marlene Ortiz-García1Blanca Patricia Lazalde-Ramos2Guillermo Moisés Zúñiga-González3Belinda Claudia Gómez-Meda4Cristina Hermila Martínez-Bugarín5Susana Vanessa Sánchez-De-La-Rosa6Saulo Oswaldo Sánchez-Rivera7Gabriela Morales-Velazquez8Celia Guerrero-Velázquez9Ana Lourdes Zamora-Perez10Instituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraInstituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraMaestría en Ciencias y Tecnología Química, Unidad Académica de Ciencias Químicas, Universidad Autónoma de ZacatecasLaboratorio de Mutagenesis, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro SocialInstituto de Genética Humana “Enrique Corona Rivera” Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraInstituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraInstituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraInstituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraInstituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraInstituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraInstituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraAbstract Background Mercury (Hg) is the major component of dental amalgam which has been utilized for decades because it is durable, inexpensive and easy to manipulate and position, as well as having a relatively low cost. Hg from dental amalgam fillings has been shown to be released into the buccal cavity due to processes such as abrasion and corrosion, and which could represent a continuous source of oxidative damage to mouth tissues. Therefore, this study assessed the impact of Hg-containing dental amalgams exposure on the oxidative genomic damage using a means of the buccal micronucleus cytome assay by counting the nuclear abnormalities (NAs) in buccal mucosa cells and by analyzing in whole saliva the molecules 8-hydroxy-2′-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) in whole saliva. Methods This study was conducted on 120 healthy children divided into two groups-group 1 (or the control group) (n = 60), including participants without dental caries, no history of dental amalgam (Hg-containing) restorations, and no other dental treatments, and group 2 (the intervention group) (n = 60), including participants with dental caries involving enamel or dentin, who received dental amalgam restorations in two posterior teeth. Oral epithelial cell and whole saliva samples were taken at baseline and 30 days later for group 1, while for group 2, samples were obtained at different pre-treatment (baseline) and post-amalgam placement time points at 15 and 30 days. Results An increased frequency of NAs (p < 0.05) and higher levels of 8-OHdG (p < 0.05) and MDA (p < 0.05) were observed after Hg-dental amalgams exposure. In addition, a positive correlation existed between NAs and oxidative stress induced by Hg from dental amalgam. Conclusions The presents study shows that individuals exposed to Hg-containing dental amalgam exhibit increased NAs in oral epithelial cells, as well as increased levels of 8-OHdG and MDA in saliva, which are directly related to genotoxicity, oxidative DNA damage and lipid peroxidation, respectively. Exposure to Hg- containing dental amalgams may contribute to oxidative stress and genetic damage, potentially leading to long-term implications for both oral and systemic health.https://doi.org/10.1186/s12903-025-06522-0DNA damageOxidative stressDental amalgamBuccal mucosa cellsNuclear abnormalitiesMercury |
| spellingShingle | Guadalupe Nalleli Macías-Lamas Yveth Marlene Ortiz-García Blanca Patricia Lazalde-Ramos Guillermo Moisés Zúñiga-González Belinda Claudia Gómez-Meda Cristina Hermila Martínez-Bugarín Susana Vanessa Sánchez-De-La-Rosa Saulo Oswaldo Sánchez-Rivera Gabriela Morales-Velazquez Celia Guerrero-Velázquez Ana Lourdes Zamora-Perez Oxidative genomic damage in pediatric patients exposed to mercury released by dental amalgam BMC Oral Health DNA damage Oxidative stress Dental amalgam Buccal mucosa cells Nuclear abnormalities Mercury |
| title | Oxidative genomic damage in pediatric patients exposed to mercury released by dental amalgam |
| title_full | Oxidative genomic damage in pediatric patients exposed to mercury released by dental amalgam |
| title_fullStr | Oxidative genomic damage in pediatric patients exposed to mercury released by dental amalgam |
| title_full_unstemmed | Oxidative genomic damage in pediatric patients exposed to mercury released by dental amalgam |
| title_short | Oxidative genomic damage in pediatric patients exposed to mercury released by dental amalgam |
| title_sort | oxidative genomic damage in pediatric patients exposed to mercury released by dental amalgam |
| topic | DNA damage Oxidative stress Dental amalgam Buccal mucosa cells Nuclear abnormalities Mercury |
| url | https://doi.org/10.1186/s12903-025-06522-0 |
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