Exploring northeast India’s culturable soil Actinomycetia for potent antibacterial agents against gram-positive bacterial pathogens of clinical importance
Abstract This study investigated the isolation and bioactivity of Actinomycetia from the soil of Northeast India, a region rich in microbial diversity. A total of 187 presumptive Actinomycetia isolates were obtained and 53 were found to exhibit antimicrobial properties. Phylogenetic analysis based o...
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Nature Portfolio
2024-11-01
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| author | Aditya Narayan Konwar Surajit Basak Shalini Gurumayum Jagat Chandra Borah Debajit Thakur |
| author_facet | Aditya Narayan Konwar Surajit Basak Shalini Gurumayum Jagat Chandra Borah Debajit Thakur |
| author_sort | Aditya Narayan Konwar |
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| description | Abstract This study investigated the isolation and bioactivity of Actinomycetia from the soil of Northeast India, a region rich in microbial diversity. A total of 187 presumptive Actinomycetia isolates were obtained and 53 were found to exhibit antimicrobial properties. Phylogenetic analysis based on 16 S rRNA gene sequencing revealed that the isolates were predominantly from the genus Streptomyces. Among these, the strain Streptomyces sp. NP14 (ANP14ARS) demonstrated specific and significant antimicrobial activity against gram-positive bacterial pathogens. The strain was further assayed against a panel of clinically important bacterial pathogens including Methicillin-resistant Staphylococcus aureus (MRSA). Its antimicrobial activity was confirmed using disc diffusion and membrane disruption assay, and its minimum inhibitory concentration was determined to be ≥ 3.12 ± 0.5 µg/ml against MRSA. Chemical analyses using FTIR and GC-MS identified key bioactive compounds, including Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)- and Phenol 3,5-bis(1,1-dimethylethyl), known for their antimicrobial properties. Toxicity studies against animal liver cell lines indicated that the ethyl acetate extract was non-toxic at concentrations below 125 µg/mL, compared to Vancomycin, which was found to be hepatotoxic at similar concentrations. These findings highlight the potential of ANP14ARS as a source of bioactive natural products that is specific towards priority pathogens such as MRSA. |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
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| spelling | doaj-art-3fc047030547495ea5f6c371cf0287d62025-01-19T12:24:53ZengNature PortfolioScientific Reports2045-23222024-11-0114111510.1038/s41598-024-77644-8Exploring northeast India’s culturable soil Actinomycetia for potent antibacterial agents against gram-positive bacterial pathogens of clinical importanceAditya Narayan Konwar0Surajit Basak1Shalini Gurumayum2Jagat Chandra Borah3Debajit Thakur4Microbial Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Studies in Science and TechnologyMicrobial Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Studies in Science and TechnologyChemical Biology Laboratory - 1, Life Sciences Division, Institute of Advanced Studies in Science and TechnologyChemical Biology Laboratory - 1, Life Sciences Division, Institute of Advanced Studies in Science and TechnologyMicrobial Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Studies in Science and TechnologyAbstract This study investigated the isolation and bioactivity of Actinomycetia from the soil of Northeast India, a region rich in microbial diversity. A total of 187 presumptive Actinomycetia isolates were obtained and 53 were found to exhibit antimicrobial properties. Phylogenetic analysis based on 16 S rRNA gene sequencing revealed that the isolates were predominantly from the genus Streptomyces. Among these, the strain Streptomyces sp. NP14 (ANP14ARS) demonstrated specific and significant antimicrobial activity against gram-positive bacterial pathogens. The strain was further assayed against a panel of clinically important bacterial pathogens including Methicillin-resistant Staphylococcus aureus (MRSA). Its antimicrobial activity was confirmed using disc diffusion and membrane disruption assay, and its minimum inhibitory concentration was determined to be ≥ 3.12 ± 0.5 µg/ml against MRSA. Chemical analyses using FTIR and GC-MS identified key bioactive compounds, including Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)- and Phenol 3,5-bis(1,1-dimethylethyl), known for their antimicrobial properties. Toxicity studies against animal liver cell lines indicated that the ethyl acetate extract was non-toxic at concentrations below 125 µg/mL, compared to Vancomycin, which was found to be hepatotoxic at similar concentrations. These findings highlight the potential of ANP14ARS as a source of bioactive natural products that is specific towards priority pathogens such as MRSA.https://doi.org/10.1038/s41598-024-77644-8ActinomycetiaStreptomycesAntimicrobialsDrug-resistanceStaphylococcus aureusCytotoxicity |
| spellingShingle | Aditya Narayan Konwar Surajit Basak Shalini Gurumayum Jagat Chandra Borah Debajit Thakur Exploring northeast India’s culturable soil Actinomycetia for potent antibacterial agents against gram-positive bacterial pathogens of clinical importance Scientific Reports Actinomycetia Streptomyces Antimicrobials Drug-resistance Staphylococcus aureus Cytotoxicity |
| title | Exploring northeast India’s culturable soil Actinomycetia for potent antibacterial agents against gram-positive bacterial pathogens of clinical importance |
| title_full | Exploring northeast India’s culturable soil Actinomycetia for potent antibacterial agents against gram-positive bacterial pathogens of clinical importance |
| title_fullStr | Exploring northeast India’s culturable soil Actinomycetia for potent antibacterial agents against gram-positive bacterial pathogens of clinical importance |
| title_full_unstemmed | Exploring northeast India’s culturable soil Actinomycetia for potent antibacterial agents against gram-positive bacterial pathogens of clinical importance |
| title_short | Exploring northeast India’s culturable soil Actinomycetia for potent antibacterial agents against gram-positive bacterial pathogens of clinical importance |
| title_sort | exploring northeast india s culturable soil actinomycetia for potent antibacterial agents against gram positive bacterial pathogens of clinical importance |
| topic | Actinomycetia Streptomyces Antimicrobials Drug-resistance Staphylococcus aureus Cytotoxicity |
| url | https://doi.org/10.1038/s41598-024-77644-8 |
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