Pilot Study of the Delivery of Microcollimated Pars Plana External Beam Radiation in Porcine Eyes: 270-Day Analysis

Objective. To determine the dose response and toxicity threshold of micro-collimated X-rays delivered to porcine maculae by a stereotactic radiosurgical system after 270 days. Methods. Twelve eyes of six Yucatan mini-swine were randomized to receive up to 90 Gy to the retina, using an office-based t...

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Main Authors: Rishi P. Singh, E. Mark Shusterman, Darius Moshfeghi, Ronald Danis, Michael Gertner
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:Journal of Ophthalmology
Online Access:http://dx.doi.org/10.1155/2012/615214
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author Rishi P. Singh
E. Mark Shusterman
Darius Moshfeghi
Ronald Danis
Michael Gertner
author_facet Rishi P. Singh
E. Mark Shusterman
Darius Moshfeghi
Ronald Danis
Michael Gertner
author_sort Rishi P. Singh
collection DOAJ
description Objective. To determine the dose response and toxicity threshold of micro-collimated X-rays delivered to porcine maculae by a stereotactic radiosurgical system after 270 days. Methods. Twelve eyes of six Yucatan mini-swine were randomized to receive up to 90 Gy to the retina, using an office-based trans-pars plana delivery system. To determine the safety profile of this radiation delivery, ophthalmic examination, fundus photography, fluorescein angiography (FA), and spectral domain optical coherence tomography (SD-OCT) were obtained at multiple time points up to 270 days post treatment. Results. No abnormalities were noted on external examination. Cataracts were noted in 4 of 12 eyes. Dose and time-dependent changes were noted on fundus examination, FA, ICG and SD-OCT. No significant abnormalities were seen in the control, 16 Gy or 24 Gy groups using any modality. Histopathology revealed a dose response effect with no discernable lesions in the 16 Gy group. Conclusion. The X-ray delivery system precisely targets the porcine retina in vivo with little effect on surrounding structures. No ophthalmic or intracranial adverse effects were noted at clinically relevant doses at 270 days following radiation delivery.
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spelling doaj-art-3fbde09bac6d481ea5b5a734538e8d2c2025-08-20T02:08:03ZengWileyJournal of Ophthalmology2090-004X2090-00582012-01-01201210.1155/2012/615214615214Pilot Study of the Delivery of Microcollimated Pars Plana External Beam Radiation in Porcine Eyes: 270-Day AnalysisRishi P. Singh0E. Mark Shusterman1Darius Moshfeghi2Ronald Danis3Michael Gertner4Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USAOraya Therapeutics, Newark, CA 94560, USAStanford University, Palo Alto, CA 94304, USAUniversity of Wisconsin, Madison, WI 53706, USAOraya Therapeutics, Newark, CA 94560, USAObjective. To determine the dose response and toxicity threshold of micro-collimated X-rays delivered to porcine maculae by a stereotactic radiosurgical system after 270 days. Methods. Twelve eyes of six Yucatan mini-swine were randomized to receive up to 90 Gy to the retina, using an office-based trans-pars plana delivery system. To determine the safety profile of this radiation delivery, ophthalmic examination, fundus photography, fluorescein angiography (FA), and spectral domain optical coherence tomography (SD-OCT) were obtained at multiple time points up to 270 days post treatment. Results. No abnormalities were noted on external examination. Cataracts were noted in 4 of 12 eyes. Dose and time-dependent changes were noted on fundus examination, FA, ICG and SD-OCT. No significant abnormalities were seen in the control, 16 Gy or 24 Gy groups using any modality. Histopathology revealed a dose response effect with no discernable lesions in the 16 Gy group. Conclusion. The X-ray delivery system precisely targets the porcine retina in vivo with little effect on surrounding structures. No ophthalmic or intracranial adverse effects were noted at clinically relevant doses at 270 days following radiation delivery.http://dx.doi.org/10.1155/2012/615214
spellingShingle Rishi P. Singh
E. Mark Shusterman
Darius Moshfeghi
Ronald Danis
Michael Gertner
Pilot Study of the Delivery of Microcollimated Pars Plana External Beam Radiation in Porcine Eyes: 270-Day Analysis
Journal of Ophthalmology
title Pilot Study of the Delivery of Microcollimated Pars Plana External Beam Radiation in Porcine Eyes: 270-Day Analysis
title_full Pilot Study of the Delivery of Microcollimated Pars Plana External Beam Radiation in Porcine Eyes: 270-Day Analysis
title_fullStr Pilot Study of the Delivery of Microcollimated Pars Plana External Beam Radiation in Porcine Eyes: 270-Day Analysis
title_full_unstemmed Pilot Study of the Delivery of Microcollimated Pars Plana External Beam Radiation in Porcine Eyes: 270-Day Analysis
title_short Pilot Study of the Delivery of Microcollimated Pars Plana External Beam Radiation in Porcine Eyes: 270-Day Analysis
title_sort pilot study of the delivery of microcollimated pars plana external beam radiation in porcine eyes 270 day analysis
url http://dx.doi.org/10.1155/2012/615214
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