16 S rRNA–based molecular identification of coagulase-negative Staphylococcus species in neonates with sepsis and their antibiotic resistance patterns in Ahvaz, Iran
Abstract Introduction Coagulase-negative staphylococci (CoNS) are among the leading causes of neonatal sepsis (NS). NS can be divided into two types: early-onset sepsis (EOS), which usually occurs less than 72 h after birth, and late-onset sepsis (LOS), which can occur 8 to 28 days after birth. Acco...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-03-01
|
| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-025-03809-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849761529607487488 |
|---|---|
| author | Arash Malakian Sahar Dehghan Effat Abbasi Montazeri Mohammad Reza Aramesh Masoud Dehdashtian Seyed Mohammad Hassan Aletayeb |
| author_facet | Arash Malakian Sahar Dehghan Effat Abbasi Montazeri Mohammad Reza Aramesh Masoud Dehdashtian Seyed Mohammad Hassan Aletayeb |
| author_sort | Arash Malakian |
| collection | DOAJ |
| description | Abstract Introduction Coagulase-negative staphylococci (CoNS) are among the leading causes of neonatal sepsis (NS). NS can be divided into two types: early-onset sepsis (EOS), which usually occurs less than 72 h after birth, and late-onset sepsis (LOS), which can occur 8 to 28 days after birth. According to newly published statistics, the incidence rates of EOS and LOS in neonates are 0.5–3.1% and 2–32%, respectively. This study aimed to determine the prevalence of common CoNS isolates and their antibiotic resistance patterns in NS cases in Ahvaz, Iran. Methods This cross-sectional study (October 2022-April 2023) was conducted on all neonates (0–28 days old) with NS manifestations admitted to Imam Khomeini Hospital. Blood culture samples were collected and incubated at 37 °C for 24 h. The bacterial isolates were identified via standard biochemical tests, and the Staphylococcus epidermidis strains were identified via polymerase chain reaction (PCR) of the SesC gene. The other suspected CoNS species were identified using 16 S rRNA sequencing. Results In total, 1221 blood culture bottles were collected from 1330 neonates with NS manifestations. A total of 111 (9.1%) blood cultures were positive for bacterial growth. Overall, 51 staphylococcal isolates, including 39 (76.5%) CoNS species and 12 (23.5%) S. aureus isolates, were identified. Using SesC gene PCR and 16 S rRNA sequencing, the CoNS species were as follows: 28 (71.8%) S. epidermidis, 5 (12.8%) S. hominis, 4 (10.38%) S. haemolyticus and 2 (5.1%) S. warneri. In total, the frequency of NS caused by CoNS isolates was 35.1% (n = 39/111). All CoNS isolates were methicillin resistant and presented the highest antibiotic resistance rates (100.0%) to cefoxitin, ampicillin, erythromycin, and linezolid. Conclusions This study revealed a high incidence of methicillin-resistant coagulase-negative staphylococci (MRCoNS) with high antibiotic resistance rates in NS patients from Ahvaz. To prevent the spread of these isolates in healthcare systems, measures such as monitoring the optimal use of antibiotics on the basis of the results of laboratory antibiograms seem necessary. |
| format | Article |
| id | doaj-art-3fbbe5b22e974a788c87aa0678689b92 |
| institution | DOAJ |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj-art-3fbbe5b22e974a788c87aa0678689b922025-08-20T03:06:00ZengBMCBMC Microbiology1471-21802025-03-0125111010.1186/s12866-025-03809-816 S rRNA–based molecular identification of coagulase-negative Staphylococcus species in neonates with sepsis and their antibiotic resistance patterns in Ahvaz, IranArash Malakian0Sahar Dehghan1Effat Abbasi Montazeri2Mohammad Reza Aramesh3Masoud Dehdashtian4Seyed Mohammad Hassan Aletayeb5Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical SciencesDepartment of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical SciencesInfectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical SciencesInfectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical SciencesInfectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical SciencesInfectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical SciencesAbstract Introduction Coagulase-negative staphylococci (CoNS) are among the leading causes of neonatal sepsis (NS). NS can be divided into two types: early-onset sepsis (EOS), which usually occurs less than 72 h after birth, and late-onset sepsis (LOS), which can occur 8 to 28 days after birth. According to newly published statistics, the incidence rates of EOS and LOS in neonates are 0.5–3.1% and 2–32%, respectively. This study aimed to determine the prevalence of common CoNS isolates and their antibiotic resistance patterns in NS cases in Ahvaz, Iran. Methods This cross-sectional study (October 2022-April 2023) was conducted on all neonates (0–28 days old) with NS manifestations admitted to Imam Khomeini Hospital. Blood culture samples were collected and incubated at 37 °C for 24 h. The bacterial isolates were identified via standard biochemical tests, and the Staphylococcus epidermidis strains were identified via polymerase chain reaction (PCR) of the SesC gene. The other suspected CoNS species were identified using 16 S rRNA sequencing. Results In total, 1221 blood culture bottles were collected from 1330 neonates with NS manifestations. A total of 111 (9.1%) blood cultures were positive for bacterial growth. Overall, 51 staphylococcal isolates, including 39 (76.5%) CoNS species and 12 (23.5%) S. aureus isolates, were identified. Using SesC gene PCR and 16 S rRNA sequencing, the CoNS species were as follows: 28 (71.8%) S. epidermidis, 5 (12.8%) S. hominis, 4 (10.38%) S. haemolyticus and 2 (5.1%) S. warneri. In total, the frequency of NS caused by CoNS isolates was 35.1% (n = 39/111). All CoNS isolates were methicillin resistant and presented the highest antibiotic resistance rates (100.0%) to cefoxitin, ampicillin, erythromycin, and linezolid. Conclusions This study revealed a high incidence of methicillin-resistant coagulase-negative staphylococci (MRCoNS) with high antibiotic resistance rates in NS patients from Ahvaz. To prevent the spread of these isolates in healthcare systems, measures such as monitoring the optimal use of antibiotics on the basis of the results of laboratory antibiograms seem necessary.https://doi.org/10.1186/s12866-025-03809-8Antibiotic resistanceCoNSEarly onset sepsisLate onset sepsisNeonatal sepsis |
| spellingShingle | Arash Malakian Sahar Dehghan Effat Abbasi Montazeri Mohammad Reza Aramesh Masoud Dehdashtian Seyed Mohammad Hassan Aletayeb 16 S rRNA–based molecular identification of coagulase-negative Staphylococcus species in neonates with sepsis and their antibiotic resistance patterns in Ahvaz, Iran BMC Microbiology Antibiotic resistance CoNS Early onset sepsis Late onset sepsis Neonatal sepsis |
| title | 16 S rRNA–based molecular identification of coagulase-negative Staphylococcus species in neonates with sepsis and their antibiotic resistance patterns in Ahvaz, Iran |
| title_full | 16 S rRNA–based molecular identification of coagulase-negative Staphylococcus species in neonates with sepsis and their antibiotic resistance patterns in Ahvaz, Iran |
| title_fullStr | 16 S rRNA–based molecular identification of coagulase-negative Staphylococcus species in neonates with sepsis and their antibiotic resistance patterns in Ahvaz, Iran |
| title_full_unstemmed | 16 S rRNA–based molecular identification of coagulase-negative Staphylococcus species in neonates with sepsis and their antibiotic resistance patterns in Ahvaz, Iran |
| title_short | 16 S rRNA–based molecular identification of coagulase-negative Staphylococcus species in neonates with sepsis and their antibiotic resistance patterns in Ahvaz, Iran |
| title_sort | 16 s rrna based molecular identification of coagulase negative staphylococcus species in neonates with sepsis and their antibiotic resistance patterns in ahvaz iran |
| topic | Antibiotic resistance CoNS Early onset sepsis Late onset sepsis Neonatal sepsis |
| url | https://doi.org/10.1186/s12866-025-03809-8 |
| work_keys_str_mv | AT arashmalakian 16srrnabasedmolecularidentificationofcoagulasenegativestaphylococcusspeciesinneonateswithsepsisandtheirantibioticresistancepatternsinahvaziran AT sahardehghan 16srrnabasedmolecularidentificationofcoagulasenegativestaphylococcusspeciesinneonateswithsepsisandtheirantibioticresistancepatternsinahvaziran AT effatabbasimontazeri 16srrnabasedmolecularidentificationofcoagulasenegativestaphylococcusspeciesinneonateswithsepsisandtheirantibioticresistancepatternsinahvaziran AT mohammadrezaaramesh 16srrnabasedmolecularidentificationofcoagulasenegativestaphylococcusspeciesinneonateswithsepsisandtheirantibioticresistancepatternsinahvaziran AT masouddehdashtian 16srrnabasedmolecularidentificationofcoagulasenegativestaphylococcusspeciesinneonateswithsepsisandtheirantibioticresistancepatternsinahvaziran AT seyedmohammadhassanaletayeb 16srrnabasedmolecularidentificationofcoagulasenegativestaphylococcusspeciesinneonateswithsepsisandtheirantibioticresistancepatternsinahvaziran |