miR-429 suppresses cell growth and induces apoptosis of human thyroid cancer cell by targeting ZEB1
Thyroid cancer is now the most common endocrine malignancy and the effect of miR-429 in the development of thyroid cancer still need to be further investigated. The expression level of miR-429 was quantified by qPCR in both clinical samples and cultured cell lines. MTT, flow cytometry, migration ana...
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| Format: | Article |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2018.1564320 |
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| author | Guochang Wu Haitao Zheng Jie Xu Yawen Guo Guibin Zheng Ci Ma Shaolong Hao Xincheng Liu Huanjie Chen Shujian Wei Xicheng Song Xiaojie Wang |
| author_facet | Guochang Wu Haitao Zheng Jie Xu Yawen Guo Guibin Zheng Ci Ma Shaolong Hao Xincheng Liu Huanjie Chen Shujian Wei Xicheng Song Xiaojie Wang |
| author_sort | Guochang Wu |
| collection | DOAJ |
| description | Thyroid cancer is now the most common endocrine malignancy and the effect of miR-429 in the development of thyroid cancer still need to be further investigated. The expression level of miR-429 was quantified by qPCR in both clinical samples and cultured cell lines. MTT, flow cytometry, migration analyses and Matrigel invasion assays were conducted to test the proliferation, apoptosis, migration and invasion of MiR-429 transfection in thyroid cancer cell lines. Luciferase activity assay and western blot were conducted to detect the direct effect of miR-429 on Zinc finger E-box-binding homeobox 1 (ZEB1) expression. In this study, it was found that miR-429 was frequently decreased in thyroid cancer tissues and cell lines. Transfection of miR-429 in thyroid cancer cell lines substantially suppressed cell proliferation, migration and invasion. Besides, miR-429 up-regulation would induce apoptosis in different cell lines. ZEB1 was identified as a direct target of miR-429 and miR-429 transfection could inhibit ZEB1 by direct binding to its 3’-untranslated region (3’-UTR). In conclusion, these data indicated that miR-429 could act as a tumour suppressor miRNA and contribute to the development and progression and metastasis of thyroid cancer. |
| format | Article |
| id | doaj-art-3fb2186ec4224482bde4cbff78f896dc |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-3fb2186ec4224482bde4cbff78f896dc2025-08-20T03:51:08ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-0147154855410.1080/21691401.2018.1564320miR-429 suppresses cell growth and induces apoptosis of human thyroid cancer cell by targeting ZEB1Guochang Wu0Haitao Zheng1Jie Xu2Yawen Guo3Guibin Zheng4Ci Ma5Shaolong Hao6Xincheng Liu7Huanjie Chen8Shujian Wei9Xicheng Song10Xiaojie Wang11Department of Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaDepartment of Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaDepartment of Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaDepartment of Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaDepartment of Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaDepartment of Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaDepartment of Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaDepartment of Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaDepartment of Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaDepartment of Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaDepartment of Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, ChinaThyroid cancer is now the most common endocrine malignancy and the effect of miR-429 in the development of thyroid cancer still need to be further investigated. The expression level of miR-429 was quantified by qPCR in both clinical samples and cultured cell lines. MTT, flow cytometry, migration analyses and Matrigel invasion assays were conducted to test the proliferation, apoptosis, migration and invasion of MiR-429 transfection in thyroid cancer cell lines. Luciferase activity assay and western blot were conducted to detect the direct effect of miR-429 on Zinc finger E-box-binding homeobox 1 (ZEB1) expression. In this study, it was found that miR-429 was frequently decreased in thyroid cancer tissues and cell lines. Transfection of miR-429 in thyroid cancer cell lines substantially suppressed cell proliferation, migration and invasion. Besides, miR-429 up-regulation would induce apoptosis in different cell lines. ZEB1 was identified as a direct target of miR-429 and miR-429 transfection could inhibit ZEB1 by direct binding to its 3’-untranslated region (3’-UTR). In conclusion, these data indicated that miR-429 could act as a tumour suppressor miRNA and contribute to the development and progression and metastasis of thyroid cancer.https://www.tandfonline.com/doi/10.1080/21691401.2018.1564320miR-429thyroid cancerZinc finger E-box-binding homeobox 1ZEB1 |
| spellingShingle | Guochang Wu Haitao Zheng Jie Xu Yawen Guo Guibin Zheng Ci Ma Shaolong Hao Xincheng Liu Huanjie Chen Shujian Wei Xicheng Song Xiaojie Wang miR-429 suppresses cell growth and induces apoptosis of human thyroid cancer cell by targeting ZEB1 Artificial Cells, Nanomedicine, and Biotechnology miR-429 thyroid cancer Zinc finger E-box-binding homeobox 1 ZEB1 |
| title | miR-429 suppresses cell growth and induces apoptosis of human thyroid cancer cell by targeting ZEB1 |
| title_full | miR-429 suppresses cell growth and induces apoptosis of human thyroid cancer cell by targeting ZEB1 |
| title_fullStr | miR-429 suppresses cell growth and induces apoptosis of human thyroid cancer cell by targeting ZEB1 |
| title_full_unstemmed | miR-429 suppresses cell growth and induces apoptosis of human thyroid cancer cell by targeting ZEB1 |
| title_short | miR-429 suppresses cell growth and induces apoptosis of human thyroid cancer cell by targeting ZEB1 |
| title_sort | mir 429 suppresses cell growth and induces apoptosis of human thyroid cancer cell by targeting zeb1 |
| topic | miR-429 thyroid cancer Zinc finger E-box-binding homeobox 1 ZEB1 |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2018.1564320 |
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