Manganese Phthalocyanine-Based Magnetic Core–Shell Composites with Peroxidase Mimetic Activity for Colorimetric Detection of Ascorbic Acid and Glutathione
Ascorbic acid (AA) and glutathione (GSH) play a pivotal role in health assessment, drug development, and quality control of nutritional supplements. The development of a new and efficient method for their detection is highly desired. In this work, we fabricated magnetic core–shell nanocomposites (Fe...
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MDPI AG
2025-03-01
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| author | Junchao Qi Long Tian Yudong Pang Fengshou Wu |
| author_facet | Junchao Qi Long Tian Yudong Pang Fengshou Wu |
| author_sort | Junchao Qi |
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| description | Ascorbic acid (AA) and glutathione (GSH) play a pivotal role in health assessment, drug development, and quality control of nutritional supplements. The development of a new and efficient method for their detection is highly desired. In this work, we fabricated magnetic core–shell nanocomposites (Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs) by a one-pot hydrothermal method with citric acid and manganese tetraamino phthalocyanine (MnTAPc) as precursors. Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs exhibited enhanced peroxidase activity compared to bare Fe<sub>3</sub>O<sub>4</sub> nanoparticles, enabling catalytic oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue ox-TMB in the presence of H<sub>2</sub>O<sub>2</sub>. Leveraging the antioxidant properties of AA/GSH to reduce ox-TMB, a colorimetric assay achieved a low detection limit of 0.161 μM for AA and 0.188 μM for GSH with broad linear ranges. Moreover, this method displayed high specificity against 12 interfering substances and excellent recyclability (>90% activity after five cycles). Finally, the Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs could act as an efficient colorimetric sensor for accurately detecting AA in genuine VC tablets and GSH in whitening serums with high accuracy. Therefore, Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs exhibited great potential in bioassay applications, benefiting from their outstanding sensitivity and high recycling rate. |
| format | Article |
| id | doaj-art-3fa8e412d9bd44ab9b892cc8fac90004 |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-03-01 |
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| series | Molecules |
| spelling | doaj-art-3fa8e412d9bd44ab9b892cc8fac900042025-08-20T03:06:16ZengMDPI AGMolecules1420-30492025-03-01307148410.3390/molecules30071484Manganese Phthalocyanine-Based Magnetic Core–Shell Composites with Peroxidase Mimetic Activity for Colorimetric Detection of Ascorbic Acid and GlutathioneJunchao Qi0Long Tian1Yudong Pang2Fengshou Wu3Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, Key Laboratory of Novel Biomass-Based Environmental and Energy Materials in Petroleum and Chemical Industry, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430072, ChinaHubei Key Laboratory of Novel Reactor and Green Chemical Technology, Key Laboratory of Novel Biomass-Based Environmental and Energy Materials in Petroleum and Chemical Industry, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430072, ChinaHubei Key Laboratory of Novel Reactor and Green Chemical Technology, Key Laboratory of Novel Biomass-Based Environmental and Energy Materials in Petroleum and Chemical Industry, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430072, ChinaHubei Key Laboratory of Novel Reactor and Green Chemical Technology, Key Laboratory of Novel Biomass-Based Environmental and Energy Materials in Petroleum and Chemical Industry, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430072, ChinaAscorbic acid (AA) and glutathione (GSH) play a pivotal role in health assessment, drug development, and quality control of nutritional supplements. The development of a new and efficient method for their detection is highly desired. In this work, we fabricated magnetic core–shell nanocomposites (Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs) by a one-pot hydrothermal method with citric acid and manganese tetraamino phthalocyanine (MnTAPc) as precursors. Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs exhibited enhanced peroxidase activity compared to bare Fe<sub>3</sub>O<sub>4</sub> nanoparticles, enabling catalytic oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue ox-TMB in the presence of H<sub>2</sub>O<sub>2</sub>. Leveraging the antioxidant properties of AA/GSH to reduce ox-TMB, a colorimetric assay achieved a low detection limit of 0.161 μM for AA and 0.188 μM for GSH with broad linear ranges. Moreover, this method displayed high specificity against 12 interfering substances and excellent recyclability (>90% activity after five cycles). Finally, the Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs could act as an efficient colorimetric sensor for accurately detecting AA in genuine VC tablets and GSH in whitening serums with high accuracy. Therefore, Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs exhibited great potential in bioassay applications, benefiting from their outstanding sensitivity and high recycling rate.https://www.mdpi.com/1420-3049/30/7/1484phthalocyaninenanoenzymesperoxidasemagneticcolorimetric |
| spellingShingle | Junchao Qi Long Tian Yudong Pang Fengshou Wu Manganese Phthalocyanine-Based Magnetic Core–Shell Composites with Peroxidase Mimetic Activity for Colorimetric Detection of Ascorbic Acid and Glutathione Molecules phthalocyanine nanoenzymes peroxidase magnetic colorimetric |
| title | Manganese Phthalocyanine-Based Magnetic Core–Shell Composites with Peroxidase Mimetic Activity for Colorimetric Detection of Ascorbic Acid and Glutathione |
| title_full | Manganese Phthalocyanine-Based Magnetic Core–Shell Composites with Peroxidase Mimetic Activity for Colorimetric Detection of Ascorbic Acid and Glutathione |
| title_fullStr | Manganese Phthalocyanine-Based Magnetic Core–Shell Composites with Peroxidase Mimetic Activity for Colorimetric Detection of Ascorbic Acid and Glutathione |
| title_full_unstemmed | Manganese Phthalocyanine-Based Magnetic Core–Shell Composites with Peroxidase Mimetic Activity for Colorimetric Detection of Ascorbic Acid and Glutathione |
| title_short | Manganese Phthalocyanine-Based Magnetic Core–Shell Composites with Peroxidase Mimetic Activity for Colorimetric Detection of Ascorbic Acid and Glutathione |
| title_sort | manganese phthalocyanine based magnetic core shell composites with peroxidase mimetic activity for colorimetric detection of ascorbic acid and glutathione |
| topic | phthalocyanine nanoenzymes peroxidase magnetic colorimetric |
| url | https://www.mdpi.com/1420-3049/30/7/1484 |
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