Manganese Phthalocyanine-Based Magnetic Core–Shell Composites with Peroxidase Mimetic Activity for Colorimetric Detection of Ascorbic Acid and Glutathione
Ascorbic acid (AA) and glutathione (GSH) play a pivotal role in health assessment, drug development, and quality control of nutritional supplements. The development of a new and efficient method for their detection is highly desired. In this work, we fabricated magnetic core–shell nanocomposites (Fe...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/7/1484 |
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| Summary: | Ascorbic acid (AA) and glutathione (GSH) play a pivotal role in health assessment, drug development, and quality control of nutritional supplements. The development of a new and efficient method for their detection is highly desired. In this work, we fabricated magnetic core–shell nanocomposites (Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs) by a one-pot hydrothermal method with citric acid and manganese tetraamino phthalocyanine (MnTAPc) as precursors. Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs exhibited enhanced peroxidase activity compared to bare Fe<sub>3</sub>O<sub>4</sub> nanoparticles, enabling catalytic oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue ox-TMB in the presence of H<sub>2</sub>O<sub>2</sub>. Leveraging the antioxidant properties of AA/GSH to reduce ox-TMB, a colorimetric assay achieved a low detection limit of 0.161 μM for AA and 0.188 μM for GSH with broad linear ranges. Moreover, this method displayed high specificity against 12 interfering substances and excellent recyclability (>90% activity after five cycles). Finally, the Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs could act as an efficient colorimetric sensor for accurately detecting AA in genuine VC tablets and GSH in whitening serums with high accuracy. Therefore, Fe<sub>3</sub>O<sub>4</sub>@MnPc-NDs exhibited great potential in bioassay applications, benefiting from their outstanding sensitivity and high recycling rate. |
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| ISSN: | 1420-3049 |