Impact of Drying–Wetting Cycles on Nitrification Inhibitors (DMPP and DMPSA) in a Greenhouse Experiment with Two Contrasting Mediterranean Soils
Studies of the impact of nitrification inhibitors (NIs), specifically DMPP and DMPSA, on N<sub>2</sub>O emissions during “hot moments” have produced conflicting results regarding their effectiveness after rewetting. This study aimed to clarify the effectiveness of NIs in reducing N<su...
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MDPI AG
2024-11-01
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| author | Laura Sánchez-Martin Adrián Bozal-Leorri Janaina M. Rodrigues Carmen González-Murua Pedro Aparicio Sonia García-Marco Antonio Vallejo |
| author_facet | Laura Sánchez-Martin Adrián Bozal-Leorri Janaina M. Rodrigues Carmen González-Murua Pedro Aparicio Sonia García-Marco Antonio Vallejo |
| author_sort | Laura Sánchez-Martin |
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| description | Studies of the impact of nitrification inhibitors (NIs), specifically DMPP and DMPSA, on N<sub>2</sub>O emissions during “hot moments” have produced conflicting results regarding their effectiveness after rewetting. This study aimed to clarify the effectiveness of NIs in reducing N<sub>2</sub>O emissions by assessing residual DMP concentration and its influence on ammonia-oxidizing bacteria (AOB) in two pot experiments using calcareous (Soil C, <i>Calcic Haploxerept</i>) and acidic soils (Soil A, <i>Dystric Xerochrepts</i>). Fertilizer treatments included urea (U), DMPP, and DMPSA. The experiments were divided into Phase I (water application to dry period, 44 days) and Phase II (rewetting from days 101 to 121). In both phases for Soil C, total N<sub>2</sub>O emissions were reduced by 88% and 90% for DMPP and DMPSA, respectively, compared with U alone. While in Phase I, the efficacy of NIs was linked to the regulation of AOB populations, in Phase II this group was not affected by NIs, suggesting that nitrification may not be the predominant process after rewetting. In Soil A, higher concentrations of DMP from DMPP were maintained compared to Soil C at the end of each phase. Despite this, NIs had no significant effect due to low nitrification rates and limited <i>amoA</i> gene abundance, indicating unfavorable conditions for nitrifiers. The study highlights the need to optimize NIs to reduce N<sub>2</sub>O emissions and improve nitrogen efficiency, while understanding their interactions with the soil. This knowledge is necessary in order to design fertilization strategies that improve the sustainability of agriculture under climate change. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
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| series | Agronomy |
| spelling | doaj-art-3f9e0c67ca62426c911a62b9b709280e2024-11-26T17:44:42ZengMDPI AGAgronomy2073-43952024-11-011411262010.3390/agronomy14112620Impact of Drying–Wetting Cycles on Nitrification Inhibitors (DMPP and DMPSA) in a Greenhouse Experiment with Two Contrasting Mediterranean SoilsLaura Sánchez-Martin0Adrián Bozal-Leorri1Janaina M. Rodrigues2Carmen González-Murua3Pedro Aparicio4Sonia García-Marco5Antonio Vallejo6Departamento de Química y Tecnología de Alimentos, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040 Madrid, SpainDepartment of Plant Biology and Ecology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, SpainInstitute for Multidisciplinary Research in Applied Biology (IMAB), Public University of Navarre, 31006 Pamplona, SpainDepartment of Plant Biology and Ecology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, SpainInstitute for Multidisciplinary Research in Applied Biology (IMAB), Public University of Navarre, 31006 Pamplona, SpainDepartamento de Química y Tecnología de Alimentos, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040 Madrid, SpainDepartamento de Química y Tecnología de Alimentos, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040 Madrid, SpainStudies of the impact of nitrification inhibitors (NIs), specifically DMPP and DMPSA, on N<sub>2</sub>O emissions during “hot moments” have produced conflicting results regarding their effectiveness after rewetting. This study aimed to clarify the effectiveness of NIs in reducing N<sub>2</sub>O emissions by assessing residual DMP concentration and its influence on ammonia-oxidizing bacteria (AOB) in two pot experiments using calcareous (Soil C, <i>Calcic Haploxerept</i>) and acidic soils (Soil A, <i>Dystric Xerochrepts</i>). Fertilizer treatments included urea (U), DMPP, and DMPSA. The experiments were divided into Phase I (water application to dry period, 44 days) and Phase II (rewetting from days 101 to 121). In both phases for Soil C, total N<sub>2</sub>O emissions were reduced by 88% and 90% for DMPP and DMPSA, respectively, compared with U alone. While in Phase I, the efficacy of NIs was linked to the regulation of AOB populations, in Phase II this group was not affected by NIs, suggesting that nitrification may not be the predominant process after rewetting. In Soil A, higher concentrations of DMP from DMPP were maintained compared to Soil C at the end of each phase. Despite this, NIs had no significant effect due to low nitrification rates and limited <i>amoA</i> gene abundance, indicating unfavorable conditions for nitrifiers. The study highlights the need to optimize NIs to reduce N<sub>2</sub>O emissions and improve nitrogen efficiency, while understanding their interactions with the soil. This knowledge is necessary in order to design fertilization strategies that improve the sustainability of agriculture under climate change.https://www.mdpi.com/2073-4395/14/11/2620wet–dry cyclescontrasting soilN<sub>2</sub>O emissionshot momentAOBDMPP |
| spellingShingle | Laura Sánchez-Martin Adrián Bozal-Leorri Janaina M. Rodrigues Carmen González-Murua Pedro Aparicio Sonia García-Marco Antonio Vallejo Impact of Drying–Wetting Cycles on Nitrification Inhibitors (DMPP and DMPSA) in a Greenhouse Experiment with Two Contrasting Mediterranean Soils Agronomy wet–dry cycles contrasting soil N<sub>2</sub>O emissions hot moment AOB DMPP |
| title | Impact of Drying–Wetting Cycles on Nitrification Inhibitors (DMPP and DMPSA) in a Greenhouse Experiment with Two Contrasting Mediterranean Soils |
| title_full | Impact of Drying–Wetting Cycles on Nitrification Inhibitors (DMPP and DMPSA) in a Greenhouse Experiment with Two Contrasting Mediterranean Soils |
| title_fullStr | Impact of Drying–Wetting Cycles on Nitrification Inhibitors (DMPP and DMPSA) in a Greenhouse Experiment with Two Contrasting Mediterranean Soils |
| title_full_unstemmed | Impact of Drying–Wetting Cycles on Nitrification Inhibitors (DMPP and DMPSA) in a Greenhouse Experiment with Two Contrasting Mediterranean Soils |
| title_short | Impact of Drying–Wetting Cycles on Nitrification Inhibitors (DMPP and DMPSA) in a Greenhouse Experiment with Two Contrasting Mediterranean Soils |
| title_sort | impact of drying wetting cycles on nitrification inhibitors dmpp and dmpsa in a greenhouse experiment with two contrasting mediterranean soils |
| topic | wet–dry cycles contrasting soil N<sub>2</sub>O emissions hot moment AOB DMPP |
| url | https://www.mdpi.com/2073-4395/14/11/2620 |
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