MiR-367-3p Alleviates Oxidative Stress Injury in the Ischemia/Reperfusion Injury Cell Model by Targeting Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4-mediated Keap1/Nrf2/ARE Pathway
Ischemic stroke is a debilitating central nervous disease linked to oxidative stress. Although miR-367-3p has been reported to be related to ischemic stroke, the direct evidence concerning oxidative stress remains elusive. Our study aimed to elucidate the mechanisms associated with oxidative stress...
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Wolters Kluwer Medknow Publications
2025-05-01
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| Series: | Journal of Physiological Investigation |
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| Online Access: | https://journals.lww.com/jpi/fulltext/2025/05000/mir_367_3p_alleviates_oxidative_stress_injury_in.2.aspx |
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| author | Qian He Xingwen Tian Yujie Zhang Qiong Mu |
| author_facet | Qian He Xingwen Tian Yujie Zhang Qiong Mu |
| author_sort | Qian He |
| collection | DOAJ |
| description | Ischemic stroke is a debilitating central nervous disease linked to oxidative stress. Although miR-367-3p has been reported to be related to ischemic stroke, the direct evidence concerning oxidative stress remains elusive. Our study aimed to elucidate the mechanisms associated with oxidative stress in ischemic stroke. Initially, we discovered that miR-367-3p was notably downregulated in SH-SY5Y cells induced by oxygen-glucose deprivation/reoxygenation (OGD/R). Employing the in vitro ischemia/reperfusion injury model, we further demonstrated that overexpression of miR-367-3p alleviated OGD/R-induced apoptosis, inflammation, and oxidative stress, accompanied by the activation of the Keap1/Nrf2/ARE pathway. Mechanistically, nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) was confirmed to be the target of miR-367-3p by dual-luciferase reporter assay. Moreover, the knockdown of NOX4 mimicked, while overexpression reversed the effects of miR-367-3p overexpression on OGD/R-induced oxidative stress injury and the impaired Keap1/Nrf2/ARE pathway. In conclusion, our findings indicate that miR-367-3p mitigates OGD/R-induced oxidative stress injury by activating the Keap1/Nrf2/ARE pathway through targeting NOX4. |
| format | Article |
| id | doaj-art-3f9da336933c417ca5ce64e6ec26ea7b |
| institution | DOAJ |
| issn | 2950-6344 2950-6352 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Physiological Investigation |
| spelling | doaj-art-3f9da336933c417ca5ce64e6ec26ea7b2025-08-20T03:16:26ZengWolters Kluwer Medknow PublicationsJournal of Physiological Investigation2950-63442950-63522025-05-0168314014910.4103/ejpi.EJPI-D-24-00103MiR-367-3p Alleviates Oxidative Stress Injury in the Ischemia/Reperfusion Injury Cell Model by Targeting Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4-mediated Keap1/Nrf2/ARE PathwayQian HeXingwen TianYujie ZhangQiong MuIschemic stroke is a debilitating central nervous disease linked to oxidative stress. Although miR-367-3p has been reported to be related to ischemic stroke, the direct evidence concerning oxidative stress remains elusive. Our study aimed to elucidate the mechanisms associated with oxidative stress in ischemic stroke. Initially, we discovered that miR-367-3p was notably downregulated in SH-SY5Y cells induced by oxygen-glucose deprivation/reoxygenation (OGD/R). Employing the in vitro ischemia/reperfusion injury model, we further demonstrated that overexpression of miR-367-3p alleviated OGD/R-induced apoptosis, inflammation, and oxidative stress, accompanied by the activation of the Keap1/Nrf2/ARE pathway. Mechanistically, nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) was confirmed to be the target of miR-367-3p by dual-luciferase reporter assay. Moreover, the knockdown of NOX4 mimicked, while overexpression reversed the effects of miR-367-3p overexpression on OGD/R-induced oxidative stress injury and the impaired Keap1/Nrf2/ARE pathway. In conclusion, our findings indicate that miR-367-3p mitigates OGD/R-induced oxidative stress injury by activating the Keap1/Nrf2/ARE pathway through targeting NOX4.https://journals.lww.com/jpi/fulltext/2025/05000/mir_367_3p_alleviates_oxidative_stress_injury_in.2.aspxkeap1/nrf2/are pathwaymir-367-3pnicotinamide adenine dinucleotide phosphate oxidase 4oxygen-glucose deprivation/reoxygenationoxidative stress |
| spellingShingle | Qian He Xingwen Tian Yujie Zhang Qiong Mu MiR-367-3p Alleviates Oxidative Stress Injury in the Ischemia/Reperfusion Injury Cell Model by Targeting Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4-mediated Keap1/Nrf2/ARE Pathway Journal of Physiological Investigation keap1/nrf2/are pathway mir-367-3p nicotinamide adenine dinucleotide phosphate oxidase 4 oxygen-glucose deprivation/reoxygenation oxidative stress |
| title | MiR-367-3p Alleviates Oxidative Stress Injury in the Ischemia/Reperfusion Injury Cell Model by Targeting Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4-mediated Keap1/Nrf2/ARE Pathway |
| title_full | MiR-367-3p Alleviates Oxidative Stress Injury in the Ischemia/Reperfusion Injury Cell Model by Targeting Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4-mediated Keap1/Nrf2/ARE Pathway |
| title_fullStr | MiR-367-3p Alleviates Oxidative Stress Injury in the Ischemia/Reperfusion Injury Cell Model by Targeting Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4-mediated Keap1/Nrf2/ARE Pathway |
| title_full_unstemmed | MiR-367-3p Alleviates Oxidative Stress Injury in the Ischemia/Reperfusion Injury Cell Model by Targeting Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4-mediated Keap1/Nrf2/ARE Pathway |
| title_short | MiR-367-3p Alleviates Oxidative Stress Injury in the Ischemia/Reperfusion Injury Cell Model by Targeting Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4-mediated Keap1/Nrf2/ARE Pathway |
| title_sort | mir 367 3p alleviates oxidative stress injury in the ischemia reperfusion injury cell model by targeting nicotinamide adenine dinucleotide phosphate oxidase 4 mediated keap1 nrf2 are pathway |
| topic | keap1/nrf2/are pathway mir-367-3p nicotinamide adenine dinucleotide phosphate oxidase 4 oxygen-glucose deprivation/reoxygenation oxidative stress |
| url | https://journals.lww.com/jpi/fulltext/2025/05000/mir_367_3p_alleviates_oxidative_stress_injury_in.2.aspx |
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