Unravelling the impact of linear energy transfer on micronuclei induction from proton and photon irradiation
Abstract Micronucleus (MN) formation has a strong link to radiation damage and is a common bio-dosimeter for acute radiation exposures. The percentage of cells containing MN (PCMN) has a strong relationship with dose, however variation between previous studies has made understanding the effect of li...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-09763-9 |
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| author | Charlotte J. Heaven John-William Warmenhoven Amy L. Chadwick Elham Santina Jamie Honeychurch Christine K. Schmidt Karen J. Kirkby Michael J. Merchant Nicholas T. Henthorn |
| author_facet | Charlotte J. Heaven John-William Warmenhoven Amy L. Chadwick Elham Santina Jamie Honeychurch Christine K. Schmidt Karen J. Kirkby Michael J. Merchant Nicholas T. Henthorn |
| author_sort | Charlotte J. Heaven |
| collection | DOAJ |
| description | Abstract Micronucleus (MN) formation has a strong link to radiation damage and is a common bio-dosimeter for acute radiation exposures. The percentage of cells containing MN (PCMN) has a strong relationship with dose, however variation between previous studies has made understanding the effect of linear energy transfer (LET) difficult. This study investigated the PCMN of seven cell lines in response to photon and proton irradiation at two different LETs (0.6 keV/ µm and 6.5 keV/ µm). MN production was scored via the cytokinesis block micronuclei assay. A linear relationship between dose and PCMN was noted for all cell lines and radiation types, with a large variability in the MN yield between cell lines. This dose-dependent increase in PCMN was independent of LET, with most cell lines showing similar responses to the radiation qualities. Overall, this data proposes a more complex relationship between dose, MN and LET. |
| format | Article |
| id | doaj-art-3f8adf596b084b6ba5ea23e039b2fa47 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-3f8adf596b084b6ba5ea23e039b2fa472025-08-20T03:45:24ZengNature PortfolioScientific Reports2045-23222025-07-0115111110.1038/s41598-025-09763-9Unravelling the impact of linear energy transfer on micronuclei induction from proton and photon irradiationCharlotte J. Heaven0John-William Warmenhoven1Amy L. Chadwick2Elham Santina3Jamie Honeychurch4Christine K. Schmidt5Karen J. Kirkby6Michael J. Merchant7Nicholas T. Henthorn8Manchester Academic Health Science Centre, The Christie NHS Foundation TrustManchester Academic Health Science Centre, The Christie NHS Foundation TrustManchester Academic Health Science Centre, The Christie NHS Foundation TrustManchester Academic Health Science Centre, The Christie NHS Foundation TrustDivision of Cancer Sciences, Faculty of Biology, Medicine and Health, School of Medical Sciences, The University of ManchesterDivision of Cancer Sciences, Faculty of Biology, Medicine and Health, School of Medical Sciences, The University of ManchesterDivision of Cancer Sciences, Faculty of Biology, Medicine and Health, School of Medical Sciences, The University of ManchesterDivision of Cancer Sciences, Faculty of Biology, Medicine and Health, School of Medical Sciences, The University of ManchesterManchester Academic Health Science Centre, The Christie NHS Foundation TrustAbstract Micronucleus (MN) formation has a strong link to radiation damage and is a common bio-dosimeter for acute radiation exposures. The percentage of cells containing MN (PCMN) has a strong relationship with dose, however variation between previous studies has made understanding the effect of linear energy transfer (LET) difficult. This study investigated the PCMN of seven cell lines in response to photon and proton irradiation at two different LETs (0.6 keV/ µm and 6.5 keV/ µm). MN production was scored via the cytokinesis block micronuclei assay. A linear relationship between dose and PCMN was noted for all cell lines and radiation types, with a large variability in the MN yield between cell lines. This dose-dependent increase in PCMN was independent of LET, with most cell lines showing similar responses to the radiation qualities. Overall, this data proposes a more complex relationship between dose, MN and LET.https://doi.org/10.1038/s41598-025-09763-9Micronucleicytochalasin-BRadiotherapyProton therapy, DNA damage |
| spellingShingle | Charlotte J. Heaven John-William Warmenhoven Amy L. Chadwick Elham Santina Jamie Honeychurch Christine K. Schmidt Karen J. Kirkby Michael J. Merchant Nicholas T. Henthorn Unravelling the impact of linear energy transfer on micronuclei induction from proton and photon irradiation Scientific Reports Micronuclei cytochalasin-B Radiotherapy Proton therapy, DNA damage |
| title | Unravelling the impact of linear energy transfer on micronuclei induction from proton and photon irradiation |
| title_full | Unravelling the impact of linear energy transfer on micronuclei induction from proton and photon irradiation |
| title_fullStr | Unravelling the impact of linear energy transfer on micronuclei induction from proton and photon irradiation |
| title_full_unstemmed | Unravelling the impact of linear energy transfer on micronuclei induction from proton and photon irradiation |
| title_short | Unravelling the impact of linear energy transfer on micronuclei induction from proton and photon irradiation |
| title_sort | unravelling the impact of linear energy transfer on micronuclei induction from proton and photon irradiation |
| topic | Micronuclei cytochalasin-B Radiotherapy Proton therapy, DNA damage |
| url | https://doi.org/10.1038/s41598-025-09763-9 |
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