Structural characterization of galactoglucomannan from medicinal fungus Hypocrella bambusae and its protective effects on drosophila intestinal injury
Despite traditional medicinal use, the polysaccharides of Hypocrella bambusae remain underexplored. This study aimed to characterize a novel polysaccharide HB-0.1 extracted and purified from the medicinal fungus Hypocrella bambusae and evaluate its therapeutic potential in mitigating intestinal inju...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925001392 |
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| author | Juan Fang Yin Chen Qiong Wang Liuyan Pu Jing Chen Kaiting Hong Zhanwei Zhang Jingna An |
| author_facet | Juan Fang Yin Chen Qiong Wang Liuyan Pu Jing Chen Kaiting Hong Zhanwei Zhang Jingna An |
| author_sort | Juan Fang |
| collection | DOAJ |
| description | Despite traditional medicinal use, the polysaccharides of Hypocrella bambusae remain underexplored. This study aimed to characterize a novel polysaccharide HB-0.1 extracted and purified from the medicinal fungus Hypocrella bambusae and evaluate its therapeutic potential in mitigating intestinal injury. The galactoglucomannan HB-0.1, was structurally characterized as a heteropolysaccharide with a (1→6)-α-D-mannopyranose backbone and branching side chains containing β-D-galactofuranose and α-D-glucopyranose residues. Functional evaluations in a Drosophila model demonstrated that HB-0.1 significantly mitigated SDS-induced intestinal injury, dose-dependently reducing reactive oxygen species (ROS), inhibiting intestinal stem cell (ISC) over proliferation, and prolonging lifespan. Mechanistic studies revealed that HB-0.1 regulated the JNK-MAPK signaling pathway, balancing oxidative stress and epithelial regeneration. These findings highlight the unique structural and therapeutic potential of HB-0.1 for intestinal protection and provide a basis for future development of bamboo-derived fungal polysaccharides. |
| format | Article |
| id | doaj-art-3f777b31c2b949e0aef2b4dced81868e |
| institution | DOAJ |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-3f777b31c2b949e0aef2b4dced81868e2025-08-20T02:39:35ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010080110.1016/j.carpta.2025.100801Structural characterization of galactoglucomannan from medicinal fungus Hypocrella bambusae and its protective effects on drosophila intestinal injuryJuan Fang0Yin Chen1Qiong Wang2Liuyan Pu3Jing Chen4Kaiting Hong5Zhanwei Zhang6Jingna An7Zhoushan Maternal and Child Care Hospital, Zhoushan 316000, ChinaSchool of Medicine, Zhejiang Ocean University, Zhoushan 316000, ChinaZhoushan Maternal and Child Care Hospital, Zhoushan 316000, ChinaZhoushan Maternal and Child Care Hospital, Zhoushan 316000, ChinaZhoushan Maternal and Child Care Hospital, Zhoushan 316000, ChinaZhoushan Maternal and Child Care Hospital, Zhoushan 316000, ChinaZhoushan Maternal and Child Care Hospital, Zhoushan 316000, ChinaZhoushan Maternal and Child Care Hospital, Zhoushan 316000, China; Corresponding author.Despite traditional medicinal use, the polysaccharides of Hypocrella bambusae remain underexplored. This study aimed to characterize a novel polysaccharide HB-0.1 extracted and purified from the medicinal fungus Hypocrella bambusae and evaluate its therapeutic potential in mitigating intestinal injury. The galactoglucomannan HB-0.1, was structurally characterized as a heteropolysaccharide with a (1→6)-α-D-mannopyranose backbone and branching side chains containing β-D-galactofuranose and α-D-glucopyranose residues. Functional evaluations in a Drosophila model demonstrated that HB-0.1 significantly mitigated SDS-induced intestinal injury, dose-dependently reducing reactive oxygen species (ROS), inhibiting intestinal stem cell (ISC) over proliferation, and prolonging lifespan. Mechanistic studies revealed that HB-0.1 regulated the JNK-MAPK signaling pathway, balancing oxidative stress and epithelial regeneration. These findings highlight the unique structural and therapeutic potential of HB-0.1 for intestinal protection and provide a basis for future development of bamboo-derived fungal polysaccharides.http://www.sciencedirect.com/science/article/pii/S2666893925001392PolysaccharideStructureDrosophila intestinal protection |
| spellingShingle | Juan Fang Yin Chen Qiong Wang Liuyan Pu Jing Chen Kaiting Hong Zhanwei Zhang Jingna An Structural characterization of galactoglucomannan from medicinal fungus Hypocrella bambusae and its protective effects on drosophila intestinal injury Carbohydrate Polymer Technologies and Applications Polysaccharide Structure Drosophila intestinal protection |
| title | Structural characterization of galactoglucomannan from medicinal fungus Hypocrella bambusae and its protective effects on drosophila intestinal injury |
| title_full | Structural characterization of galactoglucomannan from medicinal fungus Hypocrella bambusae and its protective effects on drosophila intestinal injury |
| title_fullStr | Structural characterization of galactoglucomannan from medicinal fungus Hypocrella bambusae and its protective effects on drosophila intestinal injury |
| title_full_unstemmed | Structural characterization of galactoglucomannan from medicinal fungus Hypocrella bambusae and its protective effects on drosophila intestinal injury |
| title_short | Structural characterization of galactoglucomannan from medicinal fungus Hypocrella bambusae and its protective effects on drosophila intestinal injury |
| title_sort | structural characterization of galactoglucomannan from medicinal fungus hypocrella bambusae and its protective effects on drosophila intestinal injury |
| topic | Polysaccharide Structure Drosophila intestinal protection |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925001392 |
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