Structural characterization of galactoglucomannan from medicinal fungus Hypocrella bambusae and its protective effects on drosophila intestinal injury

Despite traditional medicinal use, the polysaccharides of Hypocrella bambusae remain underexplored. This study aimed to characterize a novel polysaccharide HB-0.1 extracted and purified from the medicinal fungus Hypocrella bambusae and evaluate its therapeutic potential in mitigating intestinal inju...

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Main Authors: Juan Fang, Yin Chen, Qiong Wang, Liuyan Pu, Jing Chen, Kaiting Hong, Zhanwei Zhang, Jingna An
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Carbohydrate Polymer Technologies and Applications
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666893925001392
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Summary:Despite traditional medicinal use, the polysaccharides of Hypocrella bambusae remain underexplored. This study aimed to characterize a novel polysaccharide HB-0.1 extracted and purified from the medicinal fungus Hypocrella bambusae and evaluate its therapeutic potential in mitigating intestinal injury. The galactoglucomannan HB-0.1, was structurally characterized as a heteropolysaccharide with a (1→6)-α-D-mannopyranose backbone and branching side chains containing β-D-galactofuranose and α-D-glucopyranose residues. Functional evaluations in a Drosophila model demonstrated that HB-0.1 significantly mitigated SDS-induced intestinal injury, dose-dependently reducing reactive oxygen species (ROS), inhibiting intestinal stem cell (ISC) over proliferation, and prolonging lifespan. Mechanistic studies revealed that HB-0.1 regulated the JNK-MAPK signaling pathway, balancing oxidative stress and epithelial regeneration. These findings highlight the unique structural and therapeutic potential of HB-0.1 for intestinal protection and provide a basis for future development of bamboo-derived fungal polysaccharides.
ISSN:2666-8939