EXTRACTION OF COLLAGENOLYTIC ENZYME FROM FISH VISCERA BY PHASE PARTITIONING (PEG/CITRATE) AND ITS POTENTIAL FOR INDUSTRIAL APPLICATION
Internal viscera fish are potential sources of protein biomolecules of biopharmaceutical interest. However, this residue is frequently discarded inappropriately. The possibility to obtain by products of higher added value is a reality. Inside this view attention must be given to processes for the re...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Pesca
2021-03-01
|
| Series: | Boletim do Instituto de Pesca |
| Subjects: | |
| Online Access: | https://www.pesca.sp.gov.br/boletim/index.php/bip/article/view/1552/1481 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850257745206312960 |
|---|---|
| author | Jessica Costa SILVA Luiz Henrique Svintiskas LINO Márcia Nieves CARNEIRO DA CUNHA Juanize Matias da Silva BATISTA Vagne Melo OLIVEIRA Ana Lúcia Figueiredo PORTO |
| author_facet | Jessica Costa SILVA Luiz Henrique Svintiskas LINO Márcia Nieves CARNEIRO DA CUNHA Juanize Matias da Silva BATISTA Vagne Melo OLIVEIRA Ana Lúcia Figueiredo PORTO |
| author_sort | Jessica Costa SILVA |
| collection | DOAJ |
| description | Internal viscera fish are potential sources of protein biomolecules of biopharmaceutical interest. However, this residue is frequently discarded inappropriately. The possibility to obtain by products of higher added value is a reality. Inside this view attention must be given to processes for the recovery and extraction of target molecules. However, the high cost of processing these residues is one of the obstacles to their reuse; techniques that facilitate their handling and make the process cheaper are desirable, such as extraction in a two-phase aqueous system. Thus, the aim of this study was to extract collagenolytic enzymes from common snook (Centropomus undecimalis) using a two-phase aqueous system (polyethylene glycol/citrate), according to the 24 factorial design, using as variables: molar mass of PEG (MPEG), PEG concentration (CPEG), citrate concentration (CCIT), pH, still, considering purification factor (FP), partition coefficient (K), and yield (Y). The collagenolytic activity of the crude extract was 102.41 U mg-1, after partitioning, was purified 3.91 times (MPEG: 8000; CPEG: 20.0%; CCIT: 20.0% and pH 6.0). Inhibition (U mg-1) was observed in benzamidine (22.51), TLCK (21.05), TPCK (21.29), and PMSF (23.05), signaling to be a serine-protease. The results showed the advantage of this semipurification technique as concerns to the low cost of extraction and purification, adding value to the fishing source material and allocating the residues from its processing to the industry. |
| format | Article |
| id | doaj-art-3f74e54037a844669a4d4b28b9fd893c |
| institution | OA Journals |
| issn | 0046-9939 1678-2305 |
| language | English |
| publishDate | 2021-03-01 |
| publisher | Instituto de Pesca |
| record_format | Article |
| series | Boletim do Instituto de Pesca |
| spelling | doaj-art-3f74e54037a844669a4d4b28b9fd893c2025-08-20T01:56:20ZengInstituto de PescaBoletim do Instituto de Pesca0046-99391678-23052021-03-014641810.20950/1678-2305.2020.46.4.593EXTRACTION OF COLLAGENOLYTIC ENZYME FROM FISH VISCERA BY PHASE PARTITIONING (PEG/CITRATE) AND ITS POTENTIAL FOR INDUSTRIAL APPLICATIONJessica Costa SILVA0https://orcid.org/0000-0001-5358-2401Luiz Henrique Svintiskas LINO1https://orcid.org/0000-0002-0081-5862Márcia Nieves CARNEIRO DA CUNHA2https://orcid.org/0000-0003-2744-6216Juanize Matias da Silva BATISTA3https://orcid.org/0000-0001-7654-2533Vagne Melo OLIVEIRA4https://orcid.org/0000-0003-0841-1974Ana Lúcia Figueiredo PORTO5https://orcid.org/0000-0001-5561-5158Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Morfologia e Fisiologia Animal - DMFA, Laboratório de Tecnologia de Produtos Bioativos - LABTECBIO.Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Morfologia e Fisiologia Animal - DMFA, Laboratório de Tecnologia de Produtos Bioativos - LABTECBIOO.Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Morfologia e Fisiologia Animal - DMFA, Laboratório de Tecnologia de Produtos Bioativos - LABTECBIO.Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Morfologia e Fisiologia Animal - DMFA, Laboratório de Tecnologia de Produtos Bioativos - LABTECBIO.Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Morfologia e Fisiologia Animal - DMFA, Laboratório de Tecnologia de Produtos Bioativos - LABTECBIO.Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Morfologia e Fisiologia Animal - DMFA, Laboratório de Tecnologia de Produtos Bioativos - LABTECBIO.Internal viscera fish are potential sources of protein biomolecules of biopharmaceutical interest. However, this residue is frequently discarded inappropriately. The possibility to obtain by products of higher added value is a reality. Inside this view attention must be given to processes for the recovery and extraction of target molecules. However, the high cost of processing these residues is one of the obstacles to their reuse; techniques that facilitate their handling and make the process cheaper are desirable, such as extraction in a two-phase aqueous system. Thus, the aim of this study was to extract collagenolytic enzymes from common snook (Centropomus undecimalis) using a two-phase aqueous system (polyethylene glycol/citrate), according to the 24 factorial design, using as variables: molar mass of PEG (MPEG), PEG concentration (CPEG), citrate concentration (CCIT), pH, still, considering purification factor (FP), partition coefficient (K), and yield (Y). The collagenolytic activity of the crude extract was 102.41 U mg-1, after partitioning, was purified 3.91 times (MPEG: 8000; CPEG: 20.0%; CCIT: 20.0% and pH 6.0). Inhibition (U mg-1) was observed in benzamidine (22.51), TLCK (21.05), TPCK (21.29), and PMSF (23.05), signaling to be a serine-protease. The results showed the advantage of this semipurification technique as concerns to the low cost of extraction and purification, adding value to the fishing source material and allocating the residues from its processing to the industry.https://www.pesca.sp.gov.br/boletim/index.php/bip/article/view/1552/1481biotechnologycollagenaseby-productsresidue. |
| spellingShingle | Jessica Costa SILVA Luiz Henrique Svintiskas LINO Márcia Nieves CARNEIRO DA CUNHA Juanize Matias da Silva BATISTA Vagne Melo OLIVEIRA Ana Lúcia Figueiredo PORTO EXTRACTION OF COLLAGENOLYTIC ENZYME FROM FISH VISCERA BY PHASE PARTITIONING (PEG/CITRATE) AND ITS POTENTIAL FOR INDUSTRIAL APPLICATION Boletim do Instituto de Pesca biotechnology collagenase by-products residue. |
| title | EXTRACTION OF COLLAGENOLYTIC ENZYME FROM FISH VISCERA BY PHASE PARTITIONING (PEG/CITRATE) AND ITS POTENTIAL FOR INDUSTRIAL APPLICATION |
| title_full | EXTRACTION OF COLLAGENOLYTIC ENZYME FROM FISH VISCERA BY PHASE PARTITIONING (PEG/CITRATE) AND ITS POTENTIAL FOR INDUSTRIAL APPLICATION |
| title_fullStr | EXTRACTION OF COLLAGENOLYTIC ENZYME FROM FISH VISCERA BY PHASE PARTITIONING (PEG/CITRATE) AND ITS POTENTIAL FOR INDUSTRIAL APPLICATION |
| title_full_unstemmed | EXTRACTION OF COLLAGENOLYTIC ENZYME FROM FISH VISCERA BY PHASE PARTITIONING (PEG/CITRATE) AND ITS POTENTIAL FOR INDUSTRIAL APPLICATION |
| title_short | EXTRACTION OF COLLAGENOLYTIC ENZYME FROM FISH VISCERA BY PHASE PARTITIONING (PEG/CITRATE) AND ITS POTENTIAL FOR INDUSTRIAL APPLICATION |
| title_sort | extraction of collagenolytic enzyme from fish viscera by phase partitioning peg citrate and its potential for industrial application |
| topic | biotechnology collagenase by-products residue. |
| url | https://www.pesca.sp.gov.br/boletim/index.php/bip/article/view/1552/1481 |
| work_keys_str_mv | AT jessicacostasilva extractionofcollagenolyticenzymefromfishviscerabyphasepartitioningpegcitrateanditspotentialforindustrialapplication AT luizhenriquesvintiskaslino extractionofcollagenolyticenzymefromfishviscerabyphasepartitioningpegcitrateanditspotentialforindustrialapplication AT marcianievescarneirodacunha extractionofcollagenolyticenzymefromfishviscerabyphasepartitioningpegcitrateanditspotentialforindustrialapplication AT juanizematiasdasilvabatista extractionofcollagenolyticenzymefromfishviscerabyphasepartitioningpegcitrateanditspotentialforindustrialapplication AT vagnemelooliveira extractionofcollagenolyticenzymefromfishviscerabyphasepartitioningpegcitrateanditspotentialforindustrialapplication AT analuciafigueiredoporto extractionofcollagenolyticenzymefromfishviscerabyphasepartitioningpegcitrateanditspotentialforindustrialapplication |