Toxin production and drug resistance profiles of pediatric methicillin-resistant Staphylococcus aureus isolates in Tehran
Introduction: Staphylococcus aureus is known to be a major cause of skin and soft tissue infections, pneumonia and invasive diseases. In this study, attempts were made to examine the prevalence of tsst-1, eta, etb, and luk-PV genes among methicillin-resistant S. aureus (MRSA) isolated from children...
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The Journal of Infection in Developing Countries
2017-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/9360 |
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| author | Mozhgan Esmaeili Benvidi Hamidreza Houri Zohreh Ghalavand Bahram Nikmanesh Hadi Azimi Roghayeh Samadi Narges Nodeh Farahani Gita Eslami |
| author_facet | Mozhgan Esmaeili Benvidi Hamidreza Houri Zohreh Ghalavand Bahram Nikmanesh Hadi Azimi Roghayeh Samadi Narges Nodeh Farahani Gita Eslami |
| author_sort | Mozhgan Esmaeili Benvidi |
| collection | DOAJ |
| description |
Introduction: Staphylococcus aureus is known to be a major cause of skin and soft tissue infections, pneumonia and invasive diseases. In this study, attempts were made to examine the prevalence of tsst-1, eta, etb, and luk-PV genes among methicillin-resistant S. aureus (MRSA) isolated from children in Tehran.
Methodology: In the present cross-sectional study, a total of 100 MRSA were isolated from children who were referred to a pediatric hospital during 11-month period of September 2014 to August 2015. Isolates were identified using biochemical tests and then, using PCR, the isolates were tested for the presence of mecA, tsst-1, eta, etb, and luk-PV genes. Susceptibility of isolates to cefoxitin, penicillin, erythromycin, clindamycin, gentamicin, rifampin, minocycline, co-trimoxazole, linezolid, and vancomycin were evaluated using standard methods.
Results: It was found that the MRSA isolates had the greatest resistance to clindamycin (72%) and erythromycin (59%), while the lowest rates of resistance were observed to be related to minocycline (6%) and rifampin (12%). All of isolates were sensitive to vancomycin and linezolid. The mecA gene was detected in all the isolates. Moreover, luk-PV and tsst-1 were detected in 18% and 17% of the isolates, respectively. None of the isolates harbored eta and etb genes.
Conclusions: Our data provide specifications about the toxin production status of S. aureus isolates from pediatric children. The current study showed increased resistance to different antibiotics in S. aureus isolates. Therefore, to prevent multi-resistance to other antibiotic classes, it is essential to withhold prescriptions and stop unessential use of available antibiotics.
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| format | Article |
| id | doaj-art-3f7384dfb72d4db49b72522a04355e8a |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-3f7384dfb72d4db49b72522a04355e8a2025-08-20T02:57:45ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-10-01111010.3855/jidc.9360Toxin production and drug resistance profiles of pediatric methicillin-resistant Staphylococcus aureus isolates in TehranMozhgan Esmaeili Benvidi0Hamidreza Houri1Zohreh Ghalavand2Bahram Nikmanesh3Hadi Azimi4Roghayeh Samadi5Narges Nodeh Farahani6Gita Eslami7Shahid Beheshti University of Medical Sciences, Tehran, IranShahid Beheshti University of Medical Sciences, Tehran, IranShahid Beheshti University of Medical Sciences, Tehran, IranTehran University of Medical Sciences, Tehran, IranShahid Beheshti University of Medical Sciences, Tehran, IranShahid Beheshti University of Medical Sciences, Tehran, IranIran University of Medical Sciences, Tehran, IranShahid Beheshti University of Medical Sciences, Tehran, Iran Introduction: Staphylococcus aureus is known to be a major cause of skin and soft tissue infections, pneumonia and invasive diseases. In this study, attempts were made to examine the prevalence of tsst-1, eta, etb, and luk-PV genes among methicillin-resistant S. aureus (MRSA) isolated from children in Tehran. Methodology: In the present cross-sectional study, a total of 100 MRSA were isolated from children who were referred to a pediatric hospital during 11-month period of September 2014 to August 2015. Isolates were identified using biochemical tests and then, using PCR, the isolates were tested for the presence of mecA, tsst-1, eta, etb, and luk-PV genes. Susceptibility of isolates to cefoxitin, penicillin, erythromycin, clindamycin, gentamicin, rifampin, minocycline, co-trimoxazole, linezolid, and vancomycin were evaluated using standard methods. Results: It was found that the MRSA isolates had the greatest resistance to clindamycin (72%) and erythromycin (59%), while the lowest rates of resistance were observed to be related to minocycline (6%) and rifampin (12%). All of isolates were sensitive to vancomycin and linezolid. The mecA gene was detected in all the isolates. Moreover, luk-PV and tsst-1 were detected in 18% and 17% of the isolates, respectively. None of the isolates harbored eta and etb genes. Conclusions: Our data provide specifications about the toxin production status of S. aureus isolates from pediatric children. The current study showed increased resistance to different antibiotics in S. aureus isolates. Therefore, to prevent multi-resistance to other antibiotic classes, it is essential to withhold prescriptions and stop unessential use of available antibiotics. https://jidc.org/index.php/journal/article/view/9360Methicillin-resistant Staphylococcus aureusMethicillin-Resistant Staphylococcus aureustoxic shock syndrome toxin-1Toxic shock syndrome toxin-1Panton-Valentine leukocidinPanton-valentine leukocidin |
| spellingShingle | Mozhgan Esmaeili Benvidi Hamidreza Houri Zohreh Ghalavand Bahram Nikmanesh Hadi Azimi Roghayeh Samadi Narges Nodeh Farahani Gita Eslami Toxin production and drug resistance profiles of pediatric methicillin-resistant Staphylococcus aureus isolates in Tehran Journal of Infection in Developing Countries Methicillin-resistant Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus toxic shock syndrome toxin-1 Toxic shock syndrome toxin-1 Panton-Valentine leukocidin Panton-valentine leukocidin |
| title | Toxin production and drug resistance profiles of pediatric methicillin-resistant Staphylococcus aureus isolates in Tehran |
| title_full | Toxin production and drug resistance profiles of pediatric methicillin-resistant Staphylococcus aureus isolates in Tehran |
| title_fullStr | Toxin production and drug resistance profiles of pediatric methicillin-resistant Staphylococcus aureus isolates in Tehran |
| title_full_unstemmed | Toxin production and drug resistance profiles of pediatric methicillin-resistant Staphylococcus aureus isolates in Tehran |
| title_short | Toxin production and drug resistance profiles of pediatric methicillin-resistant Staphylococcus aureus isolates in Tehran |
| title_sort | toxin production and drug resistance profiles of pediatric methicillin resistant staphylococcus aureus isolates in tehran |
| topic | Methicillin-resistant Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus toxic shock syndrome toxin-1 Toxic shock syndrome toxin-1 Panton-Valentine leukocidin Panton-valentine leukocidin |
| url | https://jidc.org/index.php/journal/article/view/9360 |
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