Measurement of the Local Tension of Red Blood Cell Membranes by Atomic Force Spectroscopy
Objective: to study the average local tension of a membrane upon exposure to its modifiers. Materials and methods. Blood from 3 healthy donors was sampled into ethylene diamine tetraacetate-containing microvettes (Sarstedt AG and Co., Germany) during prophylactic examinations. In this series of expe...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2013-02-01
|
| Series: | Общая реаниматология |
| Online Access: | https://www.reanimatology.com/rmt/article/view/161 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Objective: to study the average local tension of a membrane upon exposure to its modifiers. Materials and methods. Blood from 3 healthy donors was sampled into ethylene diamine tetraacetate-containing microvettes (Sarstedt AG and Co., Germany) during prophylactic examinations. In this series of experiments, the red blood cells were exposed to the membrane nanosurface modifier hemin (muriatic hematin). Hemin disrupts the conformation of spectrin, a band 4.1 protein, and weakens their bond [19]. Hemin was added to blood in vitro. Its blood concentration was 1.8 mM. The images of cells and their membranes were obtained on a NTEGRA Prima atomic force microscope (NT-MDT, Russia) [16]. The membrane tension was estimated by atomic force spectroscopy. Results. After exposure to hemin, 68% of cases showed a 2.1-fold increase in the average tension as compared to the mean control value (p<0.05), which could reduce ID by «30 %. Subsequent exposure to perftoran returned the membrane tension to the baseline values in 85% of cases. The membrane tension of other 15% of the areas on the cells remained high — 2.3 times higher than the control values (p<0.05) even despite the action of perftoran. Conclusion. Thus, atomic force spectroscopy was used to measure the average local tension of the membrane, which depended on exposure to its modifiers, such as hemin. Key words: red blood cell, membrane tension, atomic force spectroscopy, hemin. |
|---|---|
| ISSN: | 1813-9779 2411-7110 |