Influence of Single-nucleotide Polymorphism of INF-γ (rs.2430561, +874 A/T) and Interleukin-10 (rs.1800896, −1082 A/G) on the Risk of Tuberculosis and Drug Resistance in Kaduna State, Nigeria
Background: Tuberculosis (TB) remains a global health challenge, necessitating comprehensive research to understand genetic factors influencing susceptibility and drug resistance. This study aimed to investigate the presence of drug resistance, analyze single nucleotide polymorphisms (SNPs) in IFN-γ...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-04-01
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| Series: | International Journal of Mycobacteriology |
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| Online Access: | https://journals.lww.com/10.4103/ijmy.ijmy_39_25 |
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| author | Suzie Madaki Yusuf Mohammed Lawal Dahiru Rogo Mustapha Yusuf Yazeed Garba Bala Umar Aliyu Ahmad |
| author_facet | Suzie Madaki Yusuf Mohammed Lawal Dahiru Rogo Mustapha Yusuf Yazeed Garba Bala Umar Aliyu Ahmad |
| author_sort | Suzie Madaki |
| collection | DOAJ |
| description | Background:
Tuberculosis (TB) remains a global health challenge, necessitating comprehensive research to understand genetic factors influencing susceptibility and drug resistance. This study aimed to investigate the presence of drug resistance, analyze single nucleotide polymorphisms (SNPs) in IFN-γ (reference SNP. 2430561, +874 Adenine/Thymine) and IL-10 (reference SNP.1800896, -1082 Adenine/Guanine), and assess their associations with age and sex among a cross section of TB patients in Kaduna state.
Methods:
A total of 140 participants, comprising drug-resistant TB (DR-TB) patients, drug-susceptible TB (DS-TB) patients, and Apparently Healthy controls (AHCs), were enrolled. Genomic deoxyribonucleic acid was extracted, and SNPs were genotyped using polymerase chain reaction-based techniques. Associations between genotypes, alleles, age, and sex were analyzed. Odd ratios and Hardy-Weinberg equilibrium were employed for demographic and genetic analyses.
Results:
In DR-TB, significant associations were observed between IFN-γ genotypes/alleles and increased susceptibility, with thymine thymine (TT) genotype and T allele showing higher frequency. For IL-10, guanine guanine (GG) genotype and G allele were prevalent, indicating potential associations with DR-TB risk. In DS-TB, similar trends were observed, highlighting potential genetic influences on susceptibility. HWE analysis revealed significant deviations in some groups, suggesting genetic variations.
Conclusions:
The prevalence of specific genotypes and alleles indicates potential genetic markers for risk assessment. Deviations from HWE suggest population-specific genetic variations. These findings underscore the importance of genetic factors in TB outcomes and advocate for tailored interventions for different populations. |
| format | Article |
| id | doaj-art-3f707d53dd5c4e1eb434fbdde01ecaf1 |
| institution | Kabale University |
| issn | 2212-5531 2212-554X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj-art-3f707d53dd5c4e1eb434fbdde01ecaf12025-08-20T03:29:19ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2025-04-0114217018110.4103/ijmy.ijmy_39_25Influence of Single-nucleotide Polymorphism of INF-γ (rs.2430561, +874 A/T) and Interleukin-10 (rs.1800896, −1082 A/G) on the Risk of Tuberculosis and Drug Resistance in Kaduna State, NigeriaSuzie MadakiYusuf MohammedLawal Dahiru RogoMustapha YusufYazeed Garba BalaUmar Aliyu AhmadBackground: Tuberculosis (TB) remains a global health challenge, necessitating comprehensive research to understand genetic factors influencing susceptibility and drug resistance. This study aimed to investigate the presence of drug resistance, analyze single nucleotide polymorphisms (SNPs) in IFN-γ (reference SNP. 2430561, +874 Adenine/Thymine) and IL-10 (reference SNP.1800896, -1082 Adenine/Guanine), and assess their associations with age and sex among a cross section of TB patients in Kaduna state. Methods: A total of 140 participants, comprising drug-resistant TB (DR-TB) patients, drug-susceptible TB (DS-TB) patients, and Apparently Healthy controls (AHCs), were enrolled. Genomic deoxyribonucleic acid was extracted, and SNPs were genotyped using polymerase chain reaction-based techniques. Associations between genotypes, alleles, age, and sex were analyzed. Odd ratios and Hardy-Weinberg equilibrium were employed for demographic and genetic analyses. Results: In DR-TB, significant associations were observed between IFN-γ genotypes/alleles and increased susceptibility, with thymine thymine (TT) genotype and T allele showing higher frequency. For IL-10, guanine guanine (GG) genotype and G allele were prevalent, indicating potential associations with DR-TB risk. In DS-TB, similar trends were observed, highlighting potential genetic influences on susceptibility. HWE analysis revealed significant deviations in some groups, suggesting genetic variations. Conclusions: The prevalence of specific genotypes and alleles indicates potential genetic markers for risk assessment. Deviations from HWE suggest population-specific genetic variations. These findings underscore the importance of genetic factors in TB outcomes and advocate for tailored interventions for different populations.https://journals.lww.com/10.4103/ijmy.ijmy_39_25interferon-gammainterleukin-10single-nucleotide polymorphismsusceptibility and drug resistancetuberculosis |
| spellingShingle | Suzie Madaki Yusuf Mohammed Lawal Dahiru Rogo Mustapha Yusuf Yazeed Garba Bala Umar Aliyu Ahmad Influence of Single-nucleotide Polymorphism of INF-γ (rs.2430561, +874 A/T) and Interleukin-10 (rs.1800896, −1082 A/G) on the Risk of Tuberculosis and Drug Resistance in Kaduna State, Nigeria International Journal of Mycobacteriology interferon-gamma interleukin-10 single-nucleotide polymorphism susceptibility and drug resistance tuberculosis |
| title | Influence of Single-nucleotide Polymorphism of INF-γ (rs.2430561, +874 A/T) and Interleukin-10 (rs.1800896, −1082 A/G) on the Risk of Tuberculosis and Drug Resistance in Kaduna State, Nigeria |
| title_full | Influence of Single-nucleotide Polymorphism of INF-γ (rs.2430561, +874 A/T) and Interleukin-10 (rs.1800896, −1082 A/G) on the Risk of Tuberculosis and Drug Resistance in Kaduna State, Nigeria |
| title_fullStr | Influence of Single-nucleotide Polymorphism of INF-γ (rs.2430561, +874 A/T) and Interleukin-10 (rs.1800896, −1082 A/G) on the Risk of Tuberculosis and Drug Resistance in Kaduna State, Nigeria |
| title_full_unstemmed | Influence of Single-nucleotide Polymorphism of INF-γ (rs.2430561, +874 A/T) and Interleukin-10 (rs.1800896, −1082 A/G) on the Risk of Tuberculosis and Drug Resistance in Kaduna State, Nigeria |
| title_short | Influence of Single-nucleotide Polymorphism of INF-γ (rs.2430561, +874 A/T) and Interleukin-10 (rs.1800896, −1082 A/G) on the Risk of Tuberculosis and Drug Resistance in Kaduna State, Nigeria |
| title_sort | influence of single nucleotide polymorphism of inf γ rs 2430561 874 a t and interleukin 10 rs 1800896 1082 a g on the risk of tuberculosis and drug resistance in kaduna state nigeria |
| topic | interferon-gamma interleukin-10 single-nucleotide polymorphism susceptibility and drug resistance tuberculosis |
| url | https://journals.lww.com/10.4103/ijmy.ijmy_39_25 |
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