Study on the response mechanism of MicroRNA novel-13 and novel-44 to Vibrio parahaemolyticus infection in Pinctada fucata martensii
Abstract Pinctada fucata martensii (P. f. martensii) is one of the main pearl oysters cultured in artificial seawater in China. However, it is highly susceptible to pathogen infection under intensive cultivation near the coast. MicroRNAs (miRNAs), as an innovative and potent regulator of immune func...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-024-04467-0 |
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| author | Chao Jie Li Qi Yuan Zhang Bin Zhang Hai Ying Liang Li Ning Ma Muhammad Salman |
| author_facet | Chao Jie Li Qi Yuan Zhang Bin Zhang Hai Ying Liang Li Ning Ma Muhammad Salman |
| author_sort | Chao Jie Li |
| collection | DOAJ |
| description | Abstract Pinctada fucata martensii (P. f. martensii) is one of the main pearl oysters cultured in artificial seawater in China. However, it is highly susceptible to pathogen infection under intensive cultivation near the coast. MicroRNAs (miRNAs), as an innovative and potent regulator of immune function, play a pivotal role in the immune response of pearl oysters to external stimuli and are a potent marker for the response of P. f. martensii to infection. This study identified two novel miRNAs, novel-13 and novel-44, from the whole transcriptome of the P. f. martensii hemocyte before and after infection with Vibrio parahaemolyticus. The dual luciferase results showed that novel-13 negatively regulated LAAO and novel-44 negatively regulated ILK. The activity of antioxidant-related enzymes increased significantly in the synthetic miRNA (novel-13 and novel-44) inhibitors and decreased significantly in the synthetic miRNA mimics. In the challenge experiment, injection with miRNA inhibitor increased the relative survival percentage by 10% compared with the control group. In conclusion, the overexpression of novel-13 and novel-44 can decrease the activity of immune and antioxidant-related enzymes, possibly affecting immune regulation in P. f. martensii by negatively regulating the LAAO and ILK target genes. |
| format | Article |
| id | doaj-art-3f68ca65b70f479eab62cad83622bfa3 |
| institution | Kabale University |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-3f68ca65b70f479eab62cad83622bfa32025-01-26T12:38:28ZengBMCBMC Veterinary Research1746-61482025-01-0121111110.1186/s12917-024-04467-0Study on the response mechanism of MicroRNA novel-13 and novel-44 to Vibrio parahaemolyticus infection in Pinctada fucata martensiiChao Jie Li0Qi Yuan Zhang1Bin Zhang2Hai Ying Liang3Li Ning Ma4Muhammad Salman5Fisheries College of Guangdong Ocean UniversityFisheries College of Guangdong Ocean UniversityFisheries College of Guangdong Ocean UniversityFisheries College of Guangdong Ocean UniversityFisheries College of Guangdong Ocean UniversityFisheries College of Guangdong Ocean UniversityAbstract Pinctada fucata martensii (P. f. martensii) is one of the main pearl oysters cultured in artificial seawater in China. However, it is highly susceptible to pathogen infection under intensive cultivation near the coast. MicroRNAs (miRNAs), as an innovative and potent regulator of immune function, play a pivotal role in the immune response of pearl oysters to external stimuli and are a potent marker for the response of P. f. martensii to infection. This study identified two novel miRNAs, novel-13 and novel-44, from the whole transcriptome of the P. f. martensii hemocyte before and after infection with Vibrio parahaemolyticus. The dual luciferase results showed that novel-13 negatively regulated LAAO and novel-44 negatively regulated ILK. The activity of antioxidant-related enzymes increased significantly in the synthetic miRNA (novel-13 and novel-44) inhibitors and decreased significantly in the synthetic miRNA mimics. In the challenge experiment, injection with miRNA inhibitor increased the relative survival percentage by 10% compared with the control group. In conclusion, the overexpression of novel-13 and novel-44 can decrease the activity of immune and antioxidant-related enzymes, possibly affecting immune regulation in P. f. martensii by negatively regulating the LAAO and ILK target genes.https://doi.org/10.1186/s12917-024-04467-0Pinctada fucata martensiimiRNAsTarget geneVibrio parahaemolyticus |
| spellingShingle | Chao Jie Li Qi Yuan Zhang Bin Zhang Hai Ying Liang Li Ning Ma Muhammad Salman Study on the response mechanism of MicroRNA novel-13 and novel-44 to Vibrio parahaemolyticus infection in Pinctada fucata martensii BMC Veterinary Research Pinctada fucata martensii miRNAs Target gene Vibrio parahaemolyticus |
| title | Study on the response mechanism of MicroRNA novel-13 and novel-44 to Vibrio parahaemolyticus infection in Pinctada fucata martensii |
| title_full | Study on the response mechanism of MicroRNA novel-13 and novel-44 to Vibrio parahaemolyticus infection in Pinctada fucata martensii |
| title_fullStr | Study on the response mechanism of MicroRNA novel-13 and novel-44 to Vibrio parahaemolyticus infection in Pinctada fucata martensii |
| title_full_unstemmed | Study on the response mechanism of MicroRNA novel-13 and novel-44 to Vibrio parahaemolyticus infection in Pinctada fucata martensii |
| title_short | Study on the response mechanism of MicroRNA novel-13 and novel-44 to Vibrio parahaemolyticus infection in Pinctada fucata martensii |
| title_sort | study on the response mechanism of microrna novel 13 and novel 44 to vibrio parahaemolyticus infection in pinctada fucata martensii |
| topic | Pinctada fucata martensii miRNAs Target gene Vibrio parahaemolyticus |
| url | https://doi.org/10.1186/s12917-024-04467-0 |
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