Multi-omics analysis of the mechanism of alfalfa and wheat-induced rumen flatulence in Xizang sheep
ABSTRACT Rumen flatulence is a diet-related rumen disease in ruminants. This study induced a rumen flatulence model in Xizang sheep using alfalfa (HRF) and wheatgrass (MRF). The aim was to understand the rumen microbiota diversity in healthy and pathological states, host-microbiota interactions, and...
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American Society for Microbiology
2025-04-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.03268-24 |
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| author | Jing Wu Xiaoming Zhang Khan Ayesha Shahzad Khuram Jianzhao Cui Gaofu Wang Zhaxi Yangzong Mingyan Shi Xunping Jiang Long Li Guiqiong Liu Wangsheng Zhao Tianzeng Song |
| author_facet | Jing Wu Xiaoming Zhang Khan Ayesha Shahzad Khuram Jianzhao Cui Gaofu Wang Zhaxi Yangzong Mingyan Shi Xunping Jiang Long Li Guiqiong Liu Wangsheng Zhao Tianzeng Song |
| author_sort | Jing Wu |
| collection | DOAJ |
| description | ABSTRACT Rumen flatulence is a diet-related rumen disease in ruminants. This study induced a rumen flatulence model in Xizang sheep using alfalfa (HRF) and wheatgrass (MRF). The aim was to understand the rumen microbiota diversity in healthy and pathological states, host-microbiota interactions, and the molecular mechanisms of rumen flatulence. Results showed that the pH in the HRF and MRF groups was lower than that in the natural grass group (LRF). SCFA concentrations varied between groups: in HRF, 2-BA and CA increased; in MRF, 4-MVA and 5-MCA rose. Microbial analysis indicated that the alpha- and beta-diversity of HRF and MRF groups were lower than LRF’s, with different microbial compositions. Transcriptome analysis revealed many differentially expressed genes (DEGs). Compared to MRF, HRF had 348 upregulated and 511 downregulated DEGs. Versus LRF, MRF had 201 upregulated and 185 downregulated DEGs, while HRF had 128 upregulated and 238 downregulated DEGs. Spearman’s correlation analysis showed that there was a positive correlation between Butyrivibrio, Quinella, and specific genes. These findings reveal the potential mechanism of rumen flatulence in Xizang sheep and provide new insights into the prevention and treatment of the disease.IMPORTANCEThe research used a high-protein diet to induce a model to understand the diversity of rumen microbiota and its interaction with the host, as well as exploring the molecular mechanisms of rumen flatulence. |
| format | Article |
| id | doaj-art-3f687a20a6614da6900ce26e43be2d72 |
| institution | OA Journals |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-3f687a20a6614da6900ce26e43be2d722025-08-20T01:54:57ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-04-0113410.1128/spectrum.03268-24Multi-omics analysis of the mechanism of alfalfa and wheat-induced rumen flatulence in Xizang sheepJing Wu0Xiaoming Zhang1Khan Ayesha2Shahzad Khuram3Jianzhao Cui4Gaofu Wang5Zhaxi Yangzong6Mingyan Shi7Xunping Jiang8Long Li9Guiqiong Liu10Wangsheng Zhao11Tianzeng Song12Institute of Animal Science, Xizang Academy of Agricultural and Animal Husbandry Science, Lhasa, Xizang, ChinaInstitute of Animal Science, Xizang Academy of Agricultural and Animal Husbandry Science, Lhasa, Xizang, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, ChinaInterdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad (CUI), Lahore, PakistanInstitute of Animal Science, Xizang Academy of Agricultural and Animal Husbandry Science, Lhasa, Xizang, ChinaChongqing Academy of Animal Science, Chongqing, ChinaInstitute of Animal Science, Xizang Academy of Agricultural and Animal Husbandry Science, Lhasa, Xizang, ChinaCollege of Life Science, Luoyang Normal University, Luoyang, Henan, ChinaCollege of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaInstitute of Animal Science, Xizang Academy of Agricultural and Animal Husbandry Science, Lhasa, Xizang, ChinaCollege of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, ChinaInstitute of Animal Science, Xizang Academy of Agricultural and Animal Husbandry Science, Lhasa, Xizang, ChinaABSTRACT Rumen flatulence is a diet-related rumen disease in ruminants. This study induced a rumen flatulence model in Xizang sheep using alfalfa (HRF) and wheatgrass (MRF). The aim was to understand the rumen microbiota diversity in healthy and pathological states, host-microbiota interactions, and the molecular mechanisms of rumen flatulence. Results showed that the pH in the HRF and MRF groups was lower than that in the natural grass group (LRF). SCFA concentrations varied between groups: in HRF, 2-BA and CA increased; in MRF, 4-MVA and 5-MCA rose. Microbial analysis indicated that the alpha- and beta-diversity of HRF and MRF groups were lower than LRF’s, with different microbial compositions. Transcriptome analysis revealed many differentially expressed genes (DEGs). Compared to MRF, HRF had 348 upregulated and 511 downregulated DEGs. Versus LRF, MRF had 201 upregulated and 185 downregulated DEGs, while HRF had 128 upregulated and 238 downregulated DEGs. Spearman’s correlation analysis showed that there was a positive correlation between Butyrivibrio, Quinella, and specific genes. These findings reveal the potential mechanism of rumen flatulence in Xizang sheep and provide new insights into the prevention and treatment of the disease.IMPORTANCEThe research used a high-protein diet to induce a model to understand the diversity of rumen microbiota and its interaction with the host, as well as exploring the molecular mechanisms of rumen flatulence.https://journals.asm.org/doi/10.1128/spectrum.03268-24rumen flatulencerumen microorganismsXizang sheepalfalfawheat |
| spellingShingle | Jing Wu Xiaoming Zhang Khan Ayesha Shahzad Khuram Jianzhao Cui Gaofu Wang Zhaxi Yangzong Mingyan Shi Xunping Jiang Long Li Guiqiong Liu Wangsheng Zhao Tianzeng Song Multi-omics analysis of the mechanism of alfalfa and wheat-induced rumen flatulence in Xizang sheep Microbiology Spectrum rumen flatulence rumen microorganisms Xizang sheep alfalfa wheat |
| title | Multi-omics analysis of the mechanism of alfalfa and wheat-induced rumen flatulence in Xizang sheep |
| title_full | Multi-omics analysis of the mechanism of alfalfa and wheat-induced rumen flatulence in Xizang sheep |
| title_fullStr | Multi-omics analysis of the mechanism of alfalfa and wheat-induced rumen flatulence in Xizang sheep |
| title_full_unstemmed | Multi-omics analysis of the mechanism of alfalfa and wheat-induced rumen flatulence in Xizang sheep |
| title_short | Multi-omics analysis of the mechanism of alfalfa and wheat-induced rumen flatulence in Xizang sheep |
| title_sort | multi omics analysis of the mechanism of alfalfa and wheat induced rumen flatulence in xizang sheep |
| topic | rumen flatulence rumen microorganisms Xizang sheep alfalfa wheat |
| url | https://journals.asm.org/doi/10.1128/spectrum.03268-24 |
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