APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis
Chloroplast stromules are dynamic tubular extensions implicated in inter-organelle communication and stress responses in plants. However, the molecular mechanisms underlying stromule-mediated processes remain largely unexplored. In this study, we investigated the localization and function of ACCLIMA...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Plant Signaling & Behavior |
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| Online Access: | http://dx.doi.org/10.1080/15592324.2025.2511830 |
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| _version_ | 1849422317361299456 |
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| author | Zihan Ge Yunhe Jing Jiaoyu Zhu Li-En Yang Shan Lu Yinyin Deng |
| author_facet | Zihan Ge Yunhe Jing Jiaoyu Zhu Li-En Yang Shan Lu Yinyin Deng |
| author_sort | Zihan Ge |
| collection | DOAJ |
| description | Chloroplast stromules are dynamic tubular extensions implicated in inter-organelle communication and stress responses in plants. However, the molecular mechanisms underlying stromule-mediated processes remain largely unexplored. In this study, we investigated the localization and function of ACCLIMATION OF PHOTOSYNTHESIS TO ENVIRONMENT1 (APE1) and its interaction with the autophagy-related protein ATG8-INTERACTING PROTEIN1 (ATI1) in Arabidopsis thaliana. Expression analysis revealed that APE1 is predominantly expressed in rosette leaves. Using yeast two-hybrid and luciferase complementation assays, we confirmed a direct interaction between APE1 and ATI1. Confocal microscopy demonstrated that APE1 localizes both within chloroplasts and stromules, co-localizing with stromule markers, while sub-organelle fractionation indicated APE1 enrichment in the thylakoid and inner envelope membranes. Our findings suggest that APE1, through its interaction with ATI1, may play a crucial role in stromule-mediated signaling and the selective autophagic degradation of chloroplast proteins during stress acclimation. This work provides new insights into the functional dynamics of stromules and highlights the importance of the APE1–ATI1 interaction in chloroplast-nucleus communication under environmental stress. |
| format | Article |
| id | doaj-art-3f66faa99a6044138a1f00a89efcd62b |
| institution | Kabale University |
| issn | 1559-2316 1559-2324 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj-art-3f66faa99a6044138a1f00a89efcd62b2025-08-20T03:31:10ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242025-12-0120110.1080/15592324.2025.25118302511830APE1 localizes to chloroplast stromules and interacts with ATI1 in ArabidopsisZihan Ge0Yunhe Jing1Jiaoyu Zhu2Li-En Yang3Shan Lu4Yinyin Deng5Nanjing UniversityNanjing UniversityNanjing UniversityJiangsu Marine Fisheries Research InstituteNanjing UniversityJiangsu Marine Fisheries Research InstituteChloroplast stromules are dynamic tubular extensions implicated in inter-organelle communication and stress responses in plants. However, the molecular mechanisms underlying stromule-mediated processes remain largely unexplored. In this study, we investigated the localization and function of ACCLIMATION OF PHOTOSYNTHESIS TO ENVIRONMENT1 (APE1) and its interaction with the autophagy-related protein ATG8-INTERACTING PROTEIN1 (ATI1) in Arabidopsis thaliana. Expression analysis revealed that APE1 is predominantly expressed in rosette leaves. Using yeast two-hybrid and luciferase complementation assays, we confirmed a direct interaction between APE1 and ATI1. Confocal microscopy demonstrated that APE1 localizes both within chloroplasts and stromules, co-localizing with stromule markers, while sub-organelle fractionation indicated APE1 enrichment in the thylakoid and inner envelope membranes. Our findings suggest that APE1, through its interaction with ATI1, may play a crucial role in stromule-mediated signaling and the selective autophagic degradation of chloroplast proteins during stress acclimation. This work provides new insights into the functional dynamics of stromules and highlights the importance of the APE1–ATI1 interaction in chloroplast-nucleus communication under environmental stress.http://dx.doi.org/10.1080/15592324.2025.2511830arabidopsis thalianaacclimation of photosynthesis to environment1 (ape1)atg8-interacting protein1 (ati1)protein–protein interactionstromule |
| spellingShingle | Zihan Ge Yunhe Jing Jiaoyu Zhu Li-En Yang Shan Lu Yinyin Deng APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis Plant Signaling & Behavior arabidopsis thaliana acclimation of photosynthesis to environment1 (ape1) atg8-interacting protein1 (ati1) protein–protein interaction stromule |
| title | APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis |
| title_full | APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis |
| title_fullStr | APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis |
| title_full_unstemmed | APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis |
| title_short | APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis |
| title_sort | ape1 localizes to chloroplast stromules and interacts with ati1 in arabidopsis |
| topic | arabidopsis thaliana acclimation of photosynthesis to environment1 (ape1) atg8-interacting protein1 (ati1) protein–protein interaction stromule |
| url | http://dx.doi.org/10.1080/15592324.2025.2511830 |
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