Advances and Application of Polyphenol Oxidase Immobilization Technology in Plants

Polyphenol oxidase (PPO) is a metalloproteinase widely present in plant organelles that plays crucial roles in photosynthesis, pest and disease resistance, growth and development, and flower color formation. Due to the high cost and reuse difficulties of plant PPO in applications, immobilization has...

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Main Authors: Fang Zhou, Haiyan Lin, Yong Luo, Changwei Liu
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/15/2335
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author Fang Zhou
Haiyan Lin
Yong Luo
Changwei Liu
author_facet Fang Zhou
Haiyan Lin
Yong Luo
Changwei Liu
author_sort Fang Zhou
collection DOAJ
description Polyphenol oxidase (PPO) is a metalloproteinase widely present in plant organelles that plays crucial roles in photosynthesis, pest and disease resistance, growth and development, and flower color formation. Due to the high cost and reuse difficulties of plant PPO in applications, immobilization has emerged as a key technology to improve its stability, recyclability, and reusability. Immobilized plant PPO has been widely used in environmental and detection fields. This review examines different immobilization methods and carrier materials for plant PPO and summarizes its applications in wastewater treatment, biosensor detection, food preservation, and theaflavin synthesis. Finally, current challenges and future opportunities for immobilized plant PPO are discussed.
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spelling doaj-art-3f5e7b393fea4fd797de9b5b592fe2782025-08-20T03:36:27ZengMDPI AGPlants2223-77472025-07-011415233510.3390/plants14152335Advances and Application of Polyphenol Oxidase Immobilization Technology in PlantsFang Zhou0Haiyan Lin1Yong Luo2Changwei Liu3School of Chemistry and Environmental Sciences, Xiangnan University, Chenzhou 423000, ChinaKey Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, ChinaSchool of Chemistry and Environmental Sciences, Xiangnan University, Chenzhou 423000, ChinaSchool of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan 411201, ChinaPolyphenol oxidase (PPO) is a metalloproteinase widely present in plant organelles that plays crucial roles in photosynthesis, pest and disease resistance, growth and development, and flower color formation. Due to the high cost and reuse difficulties of plant PPO in applications, immobilization has emerged as a key technology to improve its stability, recyclability, and reusability. Immobilized plant PPO has been widely used in environmental and detection fields. This review examines different immobilization methods and carrier materials for plant PPO and summarizes its applications in wastewater treatment, biosensor detection, food preservation, and theaflavin synthesis. Finally, current challenges and future opportunities for immobilized plant PPO are discussed.https://www.mdpi.com/2223-7747/14/15/2335plant polyphenol oxidaseimmobilization technologyimmobilization materialapplication
spellingShingle Fang Zhou
Haiyan Lin
Yong Luo
Changwei Liu
Advances and Application of Polyphenol Oxidase Immobilization Technology in Plants
Plants
plant polyphenol oxidase
immobilization technology
immobilization material
application
title Advances and Application of Polyphenol Oxidase Immobilization Technology in Plants
title_full Advances and Application of Polyphenol Oxidase Immobilization Technology in Plants
title_fullStr Advances and Application of Polyphenol Oxidase Immobilization Technology in Plants
title_full_unstemmed Advances and Application of Polyphenol Oxidase Immobilization Technology in Plants
title_short Advances and Application of Polyphenol Oxidase Immobilization Technology in Plants
title_sort advances and application of polyphenol oxidase immobilization technology in plants
topic plant polyphenol oxidase
immobilization technology
immobilization material
application
url https://www.mdpi.com/2223-7747/14/15/2335
work_keys_str_mv AT fangzhou advancesandapplicationofpolyphenoloxidaseimmobilizationtechnologyinplants
AT haiyanlin advancesandapplicationofpolyphenoloxidaseimmobilizationtechnologyinplants
AT yongluo advancesandapplicationofpolyphenoloxidaseimmobilizationtechnologyinplants
AT changweiliu advancesandapplicationofpolyphenoloxidaseimmobilizationtechnologyinplants