Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells
Objective To explore the role of pseudogene AC106872.1 in maintaining the self-renewal capacity of human embryonic stem cells (hESCs). Methods AC106872.1 was knocked out in hESCs and knockout efficiency was validated by PCR and agarose gel electrophoresis. The colony formation of hESCs was assessed...
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2025-05-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-5-561.pdf |
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| author | JIANG Zhengyang, SUN Mengyao, HE Liu, YU Jia, MA Yanni |
| author_facet | JIANG Zhengyang, SUN Mengyao, HE Liu, YU Jia, MA Yanni |
| author_sort | JIANG Zhengyang, SUN Mengyao, HE Liu, YU Jia, MA Yanni |
| collection | DOAJ |
| description | Objective To explore the role of pseudogene AC106872.1 in maintaining the self-renewal capacity of human embryonic stem cells (hESCs). Methods AC106872.1 was knocked out in hESCs and knockout efficiency was validated by PCR and agarose gel electrophoresis. The colony formation of hESCs was assessed through colony formation assays and alkaline phosphatase (AP) staining. The expression level of pluripotency and differentiation marker genes was analyzed by qPCR and flow cytometry. RNA sequencing (RNA-seq) was performed to assess transcriptomic changes upon AC106872.1 knockout. Results Knockout of AC106872.1significantly inhibited the colony formation of hESCs (P＜0.05). The expression level of pluripotency marker genes was significantly reduced (P＜0.000 1), while the expression of differentiation marker genes was markedly increased (P＜0.000 1). Conclusions The pseudogene AC106872.1 plays a crucial role in maintaining human embryonic stem cell self-renewal through regulation of pluripotency genes expression. |
| format | Article |
| id | doaj-art-3f5d6bb691304ad0a5a8e6fff32b3ed8 |
| institution | Kabale University |
| issn | 1001-6325 |
| language | zho |
| publishDate | 2025-05-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj-art-3f5d6bb691304ad0a5a8e6fff32b3ed82025-08-20T03:29:57ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252025-05-0145556156710.16352/j.issn.1001-6325.2025.05.0561Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cellsJIANG Zhengyang, SUN Mengyao, HE Liu, YU Jia, MA Yanni0Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, ChinaObjective To explore the role of pseudogene AC106872.1 in maintaining the self-renewal capacity of human embryonic stem cells (hESCs). Methods AC106872.1 was knocked out in hESCs and knockout efficiency was validated by PCR and agarose gel electrophoresis. The colony formation of hESCs was assessed through colony formation assays and alkaline phosphatase (AP) staining. The expression level of pluripotency and differentiation marker genes was analyzed by qPCR and flow cytometry. RNA sequencing (RNA-seq) was performed to assess transcriptomic changes upon AC106872.1 knockout. Results Knockout of AC106872.1significantly inhibited the colony formation of hESCs (P＜0.05). The expression level of pluripotency marker genes was significantly reduced (P＜0.000 1), while the expression of differentiation marker genes was markedly increased (P＜0.000 1). Conclusions The pseudogene AC106872.1 plays a crucial role in maintaining human embryonic stem cell self-renewal through regulation of pluripotency genes expression.https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-5-561.pdfpseudogene|human embryonic stem cells|pluripotency |
| spellingShingle | JIANG Zhengyang, SUN Mengyao, HE Liu, YU Jia, MA Yanni Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells Jichu yixue yu linchuang pseudogene|human embryonic stem cells|pluripotency |
| title | Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells |
| title_full | Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells |
| title_fullStr | Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells |
| title_full_unstemmed | Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells |
| title_short | Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells |
| title_sort | pseudogene ac106872 1 is involved in maintaining the self renewal capacity of human embryonic stem cells |
| topic | pseudogene|human embryonic stem cells|pluripotency |
| url | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-5-561.pdf |
| work_keys_str_mv | AT jiangzhengyangsunmengyaoheliuyujiamayanni pseudogeneac1068721isinvolvedinmaintainingtheselfrenewalcapacityofhumanembryonicstemcells |