The Effect of <i>G0S2</i> Gene Knockout on the Proliferation, Apoptosis, and Differentiation of Chicken Preadipocytes
The G0/G1 switch gene 2 (<i>G0S2</i>) has been shown to be involved in cell proliferation, apoptosis, and differentiation in mammals. However, its function in poultry is not fully understood, especially in preadipocytes of chickens. This study aimed to establish a <i>G0S2</i>...
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MDPI AG
2025-03-01
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| author | Yantao Li Boyu Wang Zhaochuan Wang Jintian Wen Tianle Zhou Jiahao Tang Zhenhui Li |
| author_facet | Yantao Li Boyu Wang Zhaochuan Wang Jintian Wen Tianle Zhou Jiahao Tang Zhenhui Li |
| author_sort | Yantao Li |
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| description | The G0/G1 switch gene 2 (<i>G0S2</i>) has been shown to be involved in cell proliferation, apoptosis, and differentiation in mammals. However, its function in poultry is not fully understood, especially in preadipocytes of chickens. This study aimed to establish a <i>G0S2</i> knockout preadipocyte cell line in chickens through CRISPR/Cas9 technology and to thoroughly investigate the impact of <i>G0S2</i> on chicken preadipocyte proliferation, apoptosis, and differentiation. To explore the involvement of <i>G0S2</i> in chicken preadipocyte growth and development, transcriptome sequencing was performed. The results demonstrated that <i>G0S2</i> was successfully deleted using the CRISPR/Cas9 system. <i>G0S2</i> knockout significantly inhibited the differentiation of chicken preadipocytes while promoting their proliferation. Additionally, although <i>G0S2</i> knockout exhibited a pro-apoptotic effect, it was relatively mild, primarily reflected in an increased proportion of early apoptotic cells. <i>G0S2</i> deletion significantly affected the expression of important genes related to lipid metabolism, cell cycle control, and signaling pathways, based on transcriptomic analysis. In conclusion, our findings suggest that <i>G0S2</i> performs a critical role in regulating chicken preadipocyte differentiation, proliferation, and apoptosis. This research offers valuable new insights into the molecular mechanisms and control of <i>G0S2</i> in the growth and development of chicken preadipocytes. |
| format | Article |
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| issn | 2076-2615 |
| language | English |
| publishDate | 2025-03-01 |
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| series | Animals |
| spelling | doaj-art-3f50e3aec4e24f21ba7b7493ecd0532e2025-08-20T02:17:00ZengMDPI AGAnimals2076-26152025-03-0115795110.3390/ani15070951The Effect of <i>G0S2</i> Gene Knockout on the Proliferation, Apoptosis, and Differentiation of Chicken PreadipocytesYantao Li0Boyu Wang1Zhaochuan Wang2Jintian Wen3Tianle Zhou4Jiahao Tang5Zhenhui Li6State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaThe G0/G1 switch gene 2 (<i>G0S2</i>) has been shown to be involved in cell proliferation, apoptosis, and differentiation in mammals. However, its function in poultry is not fully understood, especially in preadipocytes of chickens. This study aimed to establish a <i>G0S2</i> knockout preadipocyte cell line in chickens through CRISPR/Cas9 technology and to thoroughly investigate the impact of <i>G0S2</i> on chicken preadipocyte proliferation, apoptosis, and differentiation. To explore the involvement of <i>G0S2</i> in chicken preadipocyte growth and development, transcriptome sequencing was performed. The results demonstrated that <i>G0S2</i> was successfully deleted using the CRISPR/Cas9 system. <i>G0S2</i> knockout significantly inhibited the differentiation of chicken preadipocytes while promoting their proliferation. Additionally, although <i>G0S2</i> knockout exhibited a pro-apoptotic effect, it was relatively mild, primarily reflected in an increased proportion of early apoptotic cells. <i>G0S2</i> deletion significantly affected the expression of important genes related to lipid metabolism, cell cycle control, and signaling pathways, based on transcriptomic analysis. In conclusion, our findings suggest that <i>G0S2</i> performs a critical role in regulating chicken preadipocyte differentiation, proliferation, and apoptosis. This research offers valuable new insights into the molecular mechanisms and control of <i>G0S2</i> in the growth and development of chicken preadipocytes.https://www.mdpi.com/2076-2615/15/7/951lipocyte<i>G0S2</i>functionCRISPR/Cas9RNA-seq |
| spellingShingle | Yantao Li Boyu Wang Zhaochuan Wang Jintian Wen Tianle Zhou Jiahao Tang Zhenhui Li The Effect of <i>G0S2</i> Gene Knockout on the Proliferation, Apoptosis, and Differentiation of Chicken Preadipocytes Animals lipocyte <i>G0S2</i> function CRISPR/Cas9 RNA-seq |
| title | The Effect of <i>G0S2</i> Gene Knockout on the Proliferation, Apoptosis, and Differentiation of Chicken Preadipocytes |
| title_full | The Effect of <i>G0S2</i> Gene Knockout on the Proliferation, Apoptosis, and Differentiation of Chicken Preadipocytes |
| title_fullStr | The Effect of <i>G0S2</i> Gene Knockout on the Proliferation, Apoptosis, and Differentiation of Chicken Preadipocytes |
| title_full_unstemmed | The Effect of <i>G0S2</i> Gene Knockout on the Proliferation, Apoptosis, and Differentiation of Chicken Preadipocytes |
| title_short | The Effect of <i>G0S2</i> Gene Knockout on the Proliferation, Apoptosis, and Differentiation of Chicken Preadipocytes |
| title_sort | effect of i g0s2 i gene knockout on the proliferation apoptosis and differentiation of chicken preadipocytes |
| topic | lipocyte <i>G0S2</i> function CRISPR/Cas9 RNA-seq |
| url | https://www.mdpi.com/2076-2615/15/7/951 |
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