Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory Stimulation
Astrocytic Ca2+ dynamics have been extensively studied in ex vivo models; however, the recent development of two-photon microscopy and astrocyte-specific labeling has allowed the study of Ca2+ signaling in living central nervous system. Ca2+ waves in astrocytes have been described in cultured cells...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2012/425818 |
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| author | Giovanni Cirillo Daniele De Luca Michele Papa |
| author_facet | Giovanni Cirillo Daniele De Luca Michele Papa |
| author_sort | Giovanni Cirillo |
| collection | DOAJ |
| description | Astrocytic Ca2+ dynamics have been extensively studied in ex vivo models; however, the recent development of two-photon microscopy and astrocyte-specific labeling has allowed the study of Ca2+ signaling in living central nervous system. Ca2+ waves in astrocytes have been described in cultured cells and slice preparations, but evidence for astrocytic activation during sensory activity is lacking. There are currently few methods to image living spinal cord: breathing and heart-beating artifacts have impeded the widespread application of this technique. We here imaged the living spinal cord by two-photon microscopy in C57BL6/J mice. Through pressurized injection, we specifically loaded spinal astrocytes using the red fluorescent dye sulforhodamine 101 (SR101) and imaged astrocytic Ca2+ levels with Oregon-Green BAPTA-1 (OGB). Then, we studied astrocytic Ca2+ levels at rest and after right electrical hind paw stimulation. Sensory stimulation significantly increased astrocytic Ca2+ levels within the superficial dorsal horn of the spinal cord compared to rest. In conclusion, in vivo morphofunctional imaging of living astrocytes in spinal cord revealed that astrocytes actively participate to sensory stimulation. |
| format | Article |
| id | doaj-art-3f4f6b2952d54efba2422f05439e2e69 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-3f4f6b2952d54efba2422f05439e2e692025-08-20T03:35:54ZengWileyNeural Plasticity2090-59041687-54432012-01-01201210.1155/2012/425818425818Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory StimulationGiovanni Cirillo0Daniele De Luca1Michele Papa2Laboratorio di Morfologia delle Reti Neuronali, Dipartimento di Medicina Pubblica Clinica e Preventiva, Seconda Università di Napoli, 80100 Napoli, ItalyLaboratorio di Morfologia delle Reti Neuronali, Dipartimento di Medicina Pubblica Clinica e Preventiva, Seconda Università di Napoli, 80100 Napoli, ItalyLaboratorio di Morfologia delle Reti Neuronali, Dipartimento di Medicina Pubblica Clinica e Preventiva, Seconda Università di Napoli, 80100 Napoli, ItalyAstrocytic Ca2+ dynamics have been extensively studied in ex vivo models; however, the recent development of two-photon microscopy and astrocyte-specific labeling has allowed the study of Ca2+ signaling in living central nervous system. Ca2+ waves in astrocytes have been described in cultured cells and slice preparations, but evidence for astrocytic activation during sensory activity is lacking. There are currently few methods to image living spinal cord: breathing and heart-beating artifacts have impeded the widespread application of this technique. We here imaged the living spinal cord by two-photon microscopy in C57BL6/J mice. Through pressurized injection, we specifically loaded spinal astrocytes using the red fluorescent dye sulforhodamine 101 (SR101) and imaged astrocytic Ca2+ levels with Oregon-Green BAPTA-1 (OGB). Then, we studied astrocytic Ca2+ levels at rest and after right electrical hind paw stimulation. Sensory stimulation significantly increased astrocytic Ca2+ levels within the superficial dorsal horn of the spinal cord compared to rest. In conclusion, in vivo morphofunctional imaging of living astrocytes in spinal cord revealed that astrocytes actively participate to sensory stimulation.http://dx.doi.org/10.1155/2012/425818 |
| spellingShingle | Giovanni Cirillo Daniele De Luca Michele Papa Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory Stimulation Neural Plasticity |
| title | Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory Stimulation |
| title_full | Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory Stimulation |
| title_fullStr | Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory Stimulation |
| title_full_unstemmed | Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory Stimulation |
| title_short | Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory Stimulation |
| title_sort | calcium imaging of living astrocytes in the mouse spinal cord following sensory stimulation |
| url | http://dx.doi.org/10.1155/2012/425818 |
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