Infant feeding and treatment practices could lead to enhanced transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV) and other orally shed infections via saliva, in rural south-western Uganda
This qualitative sub-study investigated household practices affecting orally shed infections using Kaposi’s sarcoma-associated herpesvirus (KSHV) as a focus. Participants enrolled from 50 households in rural south-western Uganda were followed monthly up to three times. At enrolment, in-depth intervi...
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Taylor & Francis Group
2024-12-01
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| Series: | Global Public Health |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/17441692.2024.2418594 |
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| author | Dominic Bukenya Vickie A. Marshall Georgina Nabaggala Wendell Miley Miriam Mirembe Denise Whitby Janet Seeley Robert Newton Rosemary Rochford Katherine R. Sabourin |
| author_facet | Dominic Bukenya Vickie A. Marshall Georgina Nabaggala Wendell Miley Miriam Mirembe Denise Whitby Janet Seeley Robert Newton Rosemary Rochford Katherine R. Sabourin |
| author_sort | Dominic Bukenya |
| collection | DOAJ |
| description | This qualitative sub-study investigated household practices affecting orally shed infections using Kaposi’s sarcoma-associated herpesvirus (KSHV) as a focus. Participants enrolled from 50 households in rural south-western Uganda were followed monthly up to three times. At enrolment, in-depth interviews were completed, and venous blood collected. KSHV seropositivity was defined as anti-KSHV antibody detection to any of 25 antigens by multiplex bead-based assay. Mouthwash samples from every visit were tested by qPCR and KSHV shedders defined as individuals with KSHV DNA detected. At least one KSHV seropositive person was in 48/49(98%) households. Among those, 79% had 1+ KSHV shedders including 45% with 1+ always shedders and 92% with 1+ intermittent shedders, not mutually exclusively. All respondents reported feeding infants with pre-masticated hard food/fruits and testing food/tea temperature. Temperature was tested by tasting, pouring tea on their hand, or touching the cup to their cheek. Some cooled food/tea using a utensil or blowing over it. Food sharing amongst children and adults and using the same dish was common practice. To treat colic pain, carers/mothers reported chewing herbs and spitting into the child’s mouth. Feeding and treatment practices did not vary by KSHV status. We identified potential KSHV transmission modes in rural Ugandan households. |
| format | Article |
| id | doaj-art-3f4e382eb1bf4d8d807d792646ff0642 |
| institution | OA Journals |
| issn | 1744-1692 1744-1706 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Global Public Health |
| spelling | doaj-art-3f4e382eb1bf4d8d807d792646ff06422025-08-20T02:30:34ZengTaylor & Francis GroupGlobal Public Health1744-16921744-17062024-12-0119110.1080/17441692.2024.2418594Infant feeding and treatment practices could lead to enhanced transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV) and other orally shed infections via saliva, in rural south-western UgandaDominic Bukenya0Vickie A. Marshall1Georgina Nabaggala2Wendell Miley3Miriam Mirembe4Denise Whitby5Janet Seeley6Robert Newton7Rosemary Rochford8Katherine R. Sabourin9MRC/UVRI and LSHTM Uganda Research Unit, Entebbe, UgandaViral Oncology Section, AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Frederick, MD, USAMRC/UVRI and LSHTM Uganda Research Unit, Entebbe, UgandaViral Oncology Section, AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Frederick, MD, USAMRC/UVRI and LSHTM Uganda Research Unit, Entebbe, UgandaViral Oncology Section, AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Frederick, MD, USAMRC/UVRI and LSHTM Uganda Research Unit, Entebbe, UgandaMRC/UVRI and LSHTM Uganda Research Unit, Entebbe, UgandaDepartment of Environmental & Public Health Sciences, University of Cincinnati, Cincinnati, OH, USAMRC/UVRI and LSHTM Uganda Research Unit, Entebbe, UgandaThis qualitative sub-study investigated household practices affecting orally shed infections using Kaposi’s sarcoma-associated herpesvirus (KSHV) as a focus. Participants enrolled from 50 households in rural south-western Uganda were followed monthly up to three times. At enrolment, in-depth interviews were completed, and venous blood collected. KSHV seropositivity was defined as anti-KSHV antibody detection to any of 25 antigens by multiplex bead-based assay. Mouthwash samples from every visit were tested by qPCR and KSHV shedders defined as individuals with KSHV DNA detected. At least one KSHV seropositive person was in 48/49(98%) households. Among those, 79% had 1+ KSHV shedders including 45% with 1+ always shedders and 92% with 1+ intermittent shedders, not mutually exclusively. All respondents reported feeding infants with pre-masticated hard food/fruits and testing food/tea temperature. Temperature was tested by tasting, pouring tea on their hand, or touching the cup to their cheek. Some cooled food/tea using a utensil or blowing over it. Food sharing amongst children and adults and using the same dish was common practice. To treat colic pain, carers/mothers reported chewing herbs and spitting into the child’s mouth. Feeding and treatment practices did not vary by KSHV status. We identified potential KSHV transmission modes in rural Ugandan households.https://www.tandfonline.com/doi/10.1080/17441692.2024.2418594SDG3good health and well-beingKSHVHHV-8sheddingsaliva |
| spellingShingle | Dominic Bukenya Vickie A. Marshall Georgina Nabaggala Wendell Miley Miriam Mirembe Denise Whitby Janet Seeley Robert Newton Rosemary Rochford Katherine R. Sabourin Infant feeding and treatment practices could lead to enhanced transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV) and other orally shed infections via saliva, in rural south-western Uganda Global Public Health SDG3 good health and well-being KSHV HHV-8 shedding saliva |
| title | Infant feeding and treatment practices could lead to enhanced transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV) and other orally shed infections via saliva, in rural south-western Uganda |
| title_full | Infant feeding and treatment practices could lead to enhanced transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV) and other orally shed infections via saliva, in rural south-western Uganda |
| title_fullStr | Infant feeding and treatment practices could lead to enhanced transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV) and other orally shed infections via saliva, in rural south-western Uganda |
| title_full_unstemmed | Infant feeding and treatment practices could lead to enhanced transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV) and other orally shed infections via saliva, in rural south-western Uganda |
| title_short | Infant feeding and treatment practices could lead to enhanced transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV) and other orally shed infections via saliva, in rural south-western Uganda |
| title_sort | infant feeding and treatment practices could lead to enhanced transmission of kaposi s sarcoma associated herpesvirus kshv and other orally shed infections via saliva in rural south western uganda |
| topic | SDG3 good health and well-being KSHV HHV-8 shedding saliva |
| url | https://www.tandfonline.com/doi/10.1080/17441692.2024.2418594 |
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