Characterizing the Temporally Dynamic Nature of Relative Growth Rates: A Kinetic Analysis on Nitrogen-, Phosphorus-, and Potassium-Limited Growth

Developing precision models to describe agricultural growth is a necessary step to promote sustainable agriculture and increase resource circulation. In this study, the researchers hydroponically cultivated Bibb lettuce (<i>Lactuca sativa</i>) across a variety of nitrogen, phosphorus, an...

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Main Authors: Andrew Sharkey, Asher Altman, Yuming Sun, Thomas K. S. Igou, Yongsheng Chen
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Agriculture
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Online Access:https://www.mdpi.com/2077-0472/15/15/1641
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author Andrew Sharkey
Asher Altman
Yuming Sun
Thomas K. S. Igou
Yongsheng Chen
author_facet Andrew Sharkey
Asher Altman
Yuming Sun
Thomas K. S. Igou
Yongsheng Chen
author_sort Andrew Sharkey
collection DOAJ
description Developing precision models to describe agricultural growth is a necessary step to promote sustainable agriculture and increase resource circulation. In this study, the researchers hydroponically cultivated Bibb lettuce (<i>Lactuca sativa</i>) across a variety of nitrogen, phosphorus, and potassium (NPK)-limited treatments and developed robust data-driven kinetic models observing nutrient uptake, biomass growth, and tissue composition based on all three primary macronutrients. The resulting Dynamic μ model is the first to integrate plant maturity’s impact on growth rate, significantly improving model accuracy across limiting nutrients, treatments, and developmental stages. This reduced error supports this simple expansion as a practical and necessary inclusion for agricultural kinetic modeling. Furthermore, analysis of nutrient uptake refines the ideal hydroponic nutrient balance for Bibb lettuce to 132, 35, and 174 mg L<sup>−1</sup> (N, P, and K, respectively), while qualitative cell yield analysis identifies minimum nutrient thresholds at approximately 26.2–41.7 mg-N L<sup>−1</sup>, 3.7–5.6 mg-P L<sup>−1</sup>, and 17.4–31.5 mg-K L<sup>−1</sup> to produce compositionally healthy lettuce. These findings evaluate reclaimed wastewater’s ability to offset the fertilizer burden for lettuce by 23–45%, 14–57%, and 3–23% for N, P, and K and guide the required minimum amount of wastewater pre-processing or nutrient supplements needed to completely fulfill hydroponic nutrient demands.
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spelling doaj-art-3f4baf90f7064b70be9ff31b0bc3bfbc2025-08-20T03:36:01ZengMDPI AGAgriculture2077-04722025-07-011515164110.3390/agriculture15151641Characterizing the Temporally Dynamic Nature of Relative Growth Rates: A Kinetic Analysis on Nitrogen-, Phosphorus-, and Potassium-Limited GrowthAndrew Sharkey0Asher Altman1Yuming Sun2Thomas K. S. Igou3Yongsheng Chen4School of Civil & Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USASchool of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USASchool of Civil & Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USASchool of Civil & Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USASchool of Civil & Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USADeveloping precision models to describe agricultural growth is a necessary step to promote sustainable agriculture and increase resource circulation. In this study, the researchers hydroponically cultivated Bibb lettuce (<i>Lactuca sativa</i>) across a variety of nitrogen, phosphorus, and potassium (NPK)-limited treatments and developed robust data-driven kinetic models observing nutrient uptake, biomass growth, and tissue composition based on all three primary macronutrients. The resulting Dynamic μ model is the first to integrate plant maturity’s impact on growth rate, significantly improving model accuracy across limiting nutrients, treatments, and developmental stages. This reduced error supports this simple expansion as a practical and necessary inclusion for agricultural kinetic modeling. Furthermore, analysis of nutrient uptake refines the ideal hydroponic nutrient balance for Bibb lettuce to 132, 35, and 174 mg L<sup>−1</sup> (N, P, and K, respectively), while qualitative cell yield analysis identifies minimum nutrient thresholds at approximately 26.2–41.7 mg-N L<sup>−1</sup>, 3.7–5.6 mg-P L<sup>−1</sup>, and 17.4–31.5 mg-K L<sup>−1</sup> to produce compositionally healthy lettuce. These findings evaluate reclaimed wastewater’s ability to offset the fertilizer burden for lettuce by 23–45%, 14–57%, and 3–23% for N, P, and K and guide the required minimum amount of wastewater pre-processing or nutrient supplements needed to completely fulfill hydroponic nutrient demands.https://www.mdpi.com/2077-0472/15/15/1641MonodMichaelis–Mentenrelative growth ratespecific growth ratenutrient uptakekinetics
spellingShingle Andrew Sharkey
Asher Altman
Yuming Sun
Thomas K. S. Igou
Yongsheng Chen
Characterizing the Temporally Dynamic Nature of Relative Growth Rates: A Kinetic Analysis on Nitrogen-, Phosphorus-, and Potassium-Limited Growth
Agriculture
Monod
Michaelis–Menten
relative growth rate
specific growth rate
nutrient uptake
kinetics
title Characterizing the Temporally Dynamic Nature of Relative Growth Rates: A Kinetic Analysis on Nitrogen-, Phosphorus-, and Potassium-Limited Growth
title_full Characterizing the Temporally Dynamic Nature of Relative Growth Rates: A Kinetic Analysis on Nitrogen-, Phosphorus-, and Potassium-Limited Growth
title_fullStr Characterizing the Temporally Dynamic Nature of Relative Growth Rates: A Kinetic Analysis on Nitrogen-, Phosphorus-, and Potassium-Limited Growth
title_full_unstemmed Characterizing the Temporally Dynamic Nature of Relative Growth Rates: A Kinetic Analysis on Nitrogen-, Phosphorus-, and Potassium-Limited Growth
title_short Characterizing the Temporally Dynamic Nature of Relative Growth Rates: A Kinetic Analysis on Nitrogen-, Phosphorus-, and Potassium-Limited Growth
title_sort characterizing the temporally dynamic nature of relative growth rates a kinetic analysis on nitrogen phosphorus and potassium limited growth
topic Monod
Michaelis–Menten
relative growth rate
specific growth rate
nutrient uptake
kinetics
url https://www.mdpi.com/2077-0472/15/15/1641
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