Gut microbiota and intestinal FXR siganlling involved in the alleviation of mulberry (Morus alba L.) leaf ethanol extract on type 2 diabetes mellitus in db/db mice
The potential mechanism of mulberry leaf against type 2 diabetes mellitus (T2DM) is not fully elucidated. Here, we hypothesized that the anti-T2DM of mulberry leaf ethanol extract (MLEE) is related to improvement of gut microbiota and bile acids (BAs) metabolism. Our results showed that MLEE alone c...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624006030 |
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| author | Ding-xiang Li Sheng-qiu Xu Han Jiang Ya-Ting Li Yan-lin Zhao Peng Jin Shuai Ji Yan Du Dao-Quan Tang |
| author_facet | Ding-xiang Li Sheng-qiu Xu Han Jiang Ya-Ting Li Yan-lin Zhao Peng Jin Shuai Ji Yan Du Dao-Quan Tang |
| author_sort | Ding-xiang Li |
| collection | DOAJ |
| description | The potential mechanism of mulberry leaf against type 2 diabetes mellitus (T2DM) is not fully elucidated. Here, we hypothesized that the anti-T2DM of mulberry leaf ethanol extract (MLEE) is related to improvement of gut microbiota and bile acids (BAs) metabolism. Our results showed that MLEE alone can ameliorate glycolipid metabolism disorder, insulin resistance, and inflammation in db/db mice, and presents synergistic effects with metformin (MET). Furthermore, we found that MLEE altered the abundances of gut microbiota related to BAs metabolism such as Colidextribacter, Muribaculum, Muribaculaceae, and Eubacterium_siraeum_group, promoted fecal ωMCA level and thus inhibited intestinal farnesoid X receptor (FXR) gene expression, and then increased plasma glucagon-like peptide-1 (GLP-1) level. Furthermore, intestinal FXR inhibition and plasma GLP-1 level enhancing of MLEE could also be observed in antibiotic-intervened db/db mice. These results indicated that MLEE could alleviate T2DM via gut microbiota-BAs-intestinal FXR pathway mediated by gut microbiota and host. |
| format | Article |
| id | doaj-art-3f49f6f73c734a15967a9d0935ea6502 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-3f49f6f73c734a15967a9d0935ea65022024-12-14T06:30:19ZengElsevierJournal of Functional Foods1756-46462024-12-01123106600Gut microbiota and intestinal FXR siganlling involved in the alleviation of mulberry (Morus alba L.) leaf ethanol extract on type 2 diabetes mellitus in db/db miceDing-xiang Li0Sheng-qiu Xu1Han Jiang2Ya-Ting Li3Yan-lin Zhao4Peng Jin5Shuai Ji6Yan Du7Dao-Quan Tang8Jiangsu Province Key Laboratory of Anesthesiology, Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical University, Xuzhou 221004, ChinaDepartment of Pharmacy, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou 221116, ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, ChinaDepartment of Pharmacy, Suining People's Hospital Affiliated to Xuzhou Medical University, Suining 221202, ChinaDepartment of Pharmacy, Suining People's Hospital Affiliated to Xuzhou Medical University, Suining 221202, ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China; Department of Pharmaceutical Analysis, Xuzhou Medical University, Xuzhou 221204, ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China; Department of Pharmacy, Suining People's Hospital Affiliated to Xuzhou Medical University, Suining 221202, China; Department of Pharmaceutical Analysis, Xuzhou Medical University, Xuzhou 221204, China; Corresponding author at: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China.The potential mechanism of mulberry leaf against type 2 diabetes mellitus (T2DM) is not fully elucidated. Here, we hypothesized that the anti-T2DM of mulberry leaf ethanol extract (MLEE) is related to improvement of gut microbiota and bile acids (BAs) metabolism. Our results showed that MLEE alone can ameliorate glycolipid metabolism disorder, insulin resistance, and inflammation in db/db mice, and presents synergistic effects with metformin (MET). Furthermore, we found that MLEE altered the abundances of gut microbiota related to BAs metabolism such as Colidextribacter, Muribaculum, Muribaculaceae, and Eubacterium_siraeum_group, promoted fecal ωMCA level and thus inhibited intestinal farnesoid X receptor (FXR) gene expression, and then increased plasma glucagon-like peptide-1 (GLP-1) level. Furthermore, intestinal FXR inhibition and plasma GLP-1 level enhancing of MLEE could also be observed in antibiotic-intervened db/db mice. These results indicated that MLEE could alleviate T2DM via gut microbiota-BAs-intestinal FXR pathway mediated by gut microbiota and host.http://www.sciencedirect.com/science/article/pii/S1756464624006030Mulberry leaf ethanol extractType 2 diabetes mellitusGut microbiotaBile acidsFarnesoid X receptor |
| spellingShingle | Ding-xiang Li Sheng-qiu Xu Han Jiang Ya-Ting Li Yan-lin Zhao Peng Jin Shuai Ji Yan Du Dao-Quan Tang Gut microbiota and intestinal FXR siganlling involved in the alleviation of mulberry (Morus alba L.) leaf ethanol extract on type 2 diabetes mellitus in db/db mice Journal of Functional Foods Mulberry leaf ethanol extract Type 2 diabetes mellitus Gut microbiota Bile acids Farnesoid X receptor |
| title | Gut microbiota and intestinal FXR siganlling involved in the alleviation of mulberry (Morus alba L.) leaf ethanol extract on type 2 diabetes mellitus in db/db mice |
| title_full | Gut microbiota and intestinal FXR siganlling involved in the alleviation of mulberry (Morus alba L.) leaf ethanol extract on type 2 diabetes mellitus in db/db mice |
| title_fullStr | Gut microbiota and intestinal FXR siganlling involved in the alleviation of mulberry (Morus alba L.) leaf ethanol extract on type 2 diabetes mellitus in db/db mice |
| title_full_unstemmed | Gut microbiota and intestinal FXR siganlling involved in the alleviation of mulberry (Morus alba L.) leaf ethanol extract on type 2 diabetes mellitus in db/db mice |
| title_short | Gut microbiota and intestinal FXR siganlling involved in the alleviation of mulberry (Morus alba L.) leaf ethanol extract on type 2 diabetes mellitus in db/db mice |
| title_sort | gut microbiota and intestinal fxr siganlling involved in the alleviation of mulberry morus alba l leaf ethanol extract on type 2 diabetes mellitus in db db mice |
| topic | Mulberry leaf ethanol extract Type 2 diabetes mellitus Gut microbiota Bile acids Farnesoid X receptor |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624006030 |
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