Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry
The present study aimed to identify key metabolites related to weight reduction in humans by studying the metabolic profiles of sera obtained from 34 participants who underwent dietary intervention with black soybean peptides (BSP) for 12 weeks. This research is a sequel to our previous work in whic...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Obesity |
| Online Access: | http://dx.doi.org/10.1155/2013/874981 |
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| author | Min Jung Kim Hye Jeong Yang Jin Hee Kim Chang-Won Ahn Jong Ho Lee Kang Sung Kim Dae Young Kwon |
| author_facet | Min Jung Kim Hye Jeong Yang Jin Hee Kim Chang-Won Ahn Jong Ho Lee Kang Sung Kim Dae Young Kwon |
| author_sort | Min Jung Kim |
| collection | DOAJ |
| description | The present study aimed to identify key metabolites related to weight reduction in humans by studying the metabolic profiles of sera obtained from 34 participants who underwent dietary intervention with black soybean peptides (BSP) for 12 weeks. This research is a sequel to our previous work in which the effects of BSP on BMI and blood composition of lipid were investigated. Sera of the study were subjected to ultra performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and the data were analyzed using partial least-squares discriminate analysis (PLS-DA) score plots. Body mass index and percent body fat of the test group were reduced. Levels of betaine, benzoic acid, pyroglutamic acid, pipecolic acid, N-phenylacetamide, uric acid, l-aspartyl-l-phenylalanine, and lysophosphatidyl cholines (lysoPCs) (C18:1, C18:2, C20:1, and C20:4) showed significant increases. Levels of l-proline, valine, l-leucine/isoleucine, hypoxanthine, glutamine, l-methionine, phenylpyruvic acid, several carnitine derivatives, and lysoPCs (C14:0, PC16:0, C15:0, C16:0, C17:1, C18:0, and C22:0) were significantly decreased. In particular, lysoPC 16:0 with a VIP value of 12.02 is esteemed to be the most important metabolite for evaluating the differences between the 2 serum samples. Our result confirmed weight-lowering effects of BSP, accompanied by favorable changes in metabolites in the subjects’ blood. Therefore, this research enables us to better understand obesity and increases the predictability of the obesity-related risk by studying metabolites present in the blood. |
| format | Article |
| id | doaj-art-3f38d1ada74f4e3eb87e3172fbadf230 |
| institution | Kabale University |
| issn | 2090-0708 2090-0716 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Obesity |
| spelling | doaj-art-3f38d1ada74f4e3eb87e3172fbadf2302025-08-20T03:35:51ZengWileyJournal of Obesity2090-07082090-07162013-01-01201310.1155/2013/874981874981Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass SpectrometryMin Jung Kim0Hye Jeong Yang1Jin Hee Kim2Chang-Won Ahn3Jong Ho Lee4Kang Sung Kim5Dae Young Kwon6Department of Food Metabolism and Nutrition, Korea Food Research Institute, 516 Paekhyon-dong, Pundang-ku, Songnam, Kyongki-do 463-747, Republic of KoreaDepartment of Food Metabolism and Nutrition, Korea Food Research Institute, 516 Paekhyon-dong, Pundang-ku, Songnam, Kyongki-do 463-747, Republic of KoreaDepartment of Food Metabolism and Nutrition, Korea Food Research Institute, 516 Paekhyon-dong, Pundang-ku, Songnam, Kyongki-do 463-747, Republic of KoreaResearch and Development Center, Nong Shim Co., Ltd., Seoul 156-709, Republic of KoreaDepartment of Food & Nutrition, Yonsei University, Seoul 120-749, Republic of KoreaDepartment of Food Science & Nutrition, Yongin University, Kyongki-do, 134 Samka-dong, Chuin-ku, Yongin, Kyongki-do 449-714, Republic of KoreaDepartment of Food Metabolism and Nutrition, Korea Food Research Institute, 516 Paekhyon-dong, Pundang-ku, Songnam, Kyongki-do 463-747, Republic of KoreaThe present study aimed to identify key metabolites related to weight reduction in humans by studying the metabolic profiles of sera obtained from 34 participants who underwent dietary intervention with black soybean peptides (BSP) for 12 weeks. This research is a sequel to our previous work in which the effects of BSP on BMI and blood composition of lipid were investigated. Sera of the study were subjected to ultra performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and the data were analyzed using partial least-squares discriminate analysis (PLS-DA) score plots. Body mass index and percent body fat of the test group were reduced. Levels of betaine, benzoic acid, pyroglutamic acid, pipecolic acid, N-phenylacetamide, uric acid, l-aspartyl-l-phenylalanine, and lysophosphatidyl cholines (lysoPCs) (C18:1, C18:2, C20:1, and C20:4) showed significant increases. Levels of l-proline, valine, l-leucine/isoleucine, hypoxanthine, glutamine, l-methionine, phenylpyruvic acid, several carnitine derivatives, and lysoPCs (C14:0, PC16:0, C15:0, C16:0, C17:1, C18:0, and C22:0) were significantly decreased. In particular, lysoPC 16:0 with a VIP value of 12.02 is esteemed to be the most important metabolite for evaluating the differences between the 2 serum samples. Our result confirmed weight-lowering effects of BSP, accompanied by favorable changes in metabolites in the subjects’ blood. Therefore, this research enables us to better understand obesity and increases the predictability of the obesity-related risk by studying metabolites present in the blood.http://dx.doi.org/10.1155/2013/874981 |
| spellingShingle | Min Jung Kim Hye Jeong Yang Jin Hee Kim Chang-Won Ahn Jong Ho Lee Kang Sung Kim Dae Young Kwon Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry Journal of Obesity |
| title | Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry |
| title_full | Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry |
| title_fullStr | Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry |
| title_full_unstemmed | Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry |
| title_short | Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry |
| title_sort | obesity related metabolomic analysis of human subjects in black soybean peptide intervention study by ultraperformance liquid chromatography and quadrupole time of flight mass spectrometry |
| url | http://dx.doi.org/10.1155/2013/874981 |
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