Shenlian (SL) Decoction, a Traditional Chinese Medicine Compound, May Ameliorate Blood Glucose via Mediating the Gut Microbiota in db/db Mice
Shenlian (SL) decoction is a herbal formula composed of Coptis and ginseng, of which berberine and ginsenoside are the main constituents. Even though SL decoction is widely used in treating diabetes in China, the mechanism of its antidiabetes function still needs further study. Gut microbiota disord...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2022/7802107 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832555916290949120 |
|---|---|
| author | Rui-xi Sun Wei-jun Huang Yao Xiao Dou-dou Wang Guo-hua Mu He Nan Bo-ran Ni Xiao-qiang Huang Hsuan-chuan Wang Yi-fan Liu Qiang Fu Jin-xi Zhao |
| author_facet | Rui-xi Sun Wei-jun Huang Yao Xiao Dou-dou Wang Guo-hua Mu He Nan Bo-ran Ni Xiao-qiang Huang Hsuan-chuan Wang Yi-fan Liu Qiang Fu Jin-xi Zhao |
| author_sort | Rui-xi Sun |
| collection | DOAJ |
| description | Shenlian (SL) decoction is a herbal formula composed of Coptis and ginseng, of which berberine and ginsenoside are the main constituents. Even though SL decoction is widely used in treating diabetes in China, the mechanism of its antidiabetes function still needs further study. Gut microbiota disorder is one of the important factors that cause diabetes. To explore the effect of SL decoction on intestinal microbiota, gut microbiota of mice was analyzed by sequencing the gut bacterial 16S rRNA V3+V4 region and metagenomics. In this study, results demonstrated that SL decoction had a better hypoglycemic effect and β cell protection effect than either ginseng or Coptis chinensis. Alpha diversity analysis showed that all interventions with ginseng, Coptis, and SL decoction could reverse the increased diversity and richness of gut microbiota in db/db mice. PCoA analysis showed oral SL decoction significantly alters gut microbiota composition in db/db mice. 395 OTUs showed significant differences after SL treatment, of which 37 OTUs enriched by SL decoction showed a significant negative correlation with FBG, and 204 OTUs decreased by SL decoction showed a significant positive correlation with FBG. Results of KEGG analysis and metagenomic sequencing showed that SL decoction could reduce the Prevotellaceae, Rikenellaceae, and Helicobacteraceae, which were related to lipopolysaccharide biosynthesis, riboflavin metabolism, and peroxisome, respectively. It could also upregulate the abundance of Bacteroidaceae, which contributed to the metabolism of starch and sucrose as well as pentose-glucuronate interconversions. In the species level, SL decoction significantly upregulates the relative abundance of Bacteroides_acidifaciens which showed a significant negative correlation with FBG and was reported to be a potential agent for modulating metabolic disorders such as diabetes and obesity. In conclusion, SL decoction was effective in hypoglycemia and its mechanism may be related to regulating gut microbiota via upregulating Bacteroides_acidifaciens. |
| format | Article |
| id | doaj-art-3f35784c1cba45d287910f8e77b82cf0 |
| institution | Kabale University |
| issn | 2314-6753 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-3f35784c1cba45d287910f8e77b82cf02025-02-03T05:46:48ZengWileyJournal of Diabetes Research2314-67532022-01-01202210.1155/2022/7802107Shenlian (SL) Decoction, a Traditional Chinese Medicine Compound, May Ameliorate Blood Glucose via Mediating the Gut Microbiota in db/db MiceRui-xi Sun0Wei-jun Huang1Yao Xiao2Dou-dou Wang3Guo-hua Mu4He Nan5Bo-ran Ni6Xiao-qiang Huang7Hsuan-chuan Wang8Yi-fan Liu9Qiang Fu10Jin-xi Zhao11Beijing University of Chinese MedicineKey Laboratory of Chinese Internal Medicine of Ministry of Education and BeijingNephropathy DepartmentSection II of Endocrinology & Nephropathy DepartmentSection II of Endocrinology & Nephropathy DepartmentSection II of Endocrinology & Nephropathy DepartmentSection II of Endocrinology & Nephropathy DepartmentSection II of Endocrinology & Nephropathy DepartmentSection II of Endocrinology & Nephropathy DepartmentSection II of Endocrinology & Nephropathy DepartmentSection II of Endocrinology & Nephropathy DepartmentSection II of Endocrinology & Nephropathy DepartmentShenlian (SL) decoction is a herbal formula composed of Coptis and ginseng, of which berberine and ginsenoside are the main constituents. Even though SL decoction is widely used in treating diabetes in China, the mechanism of its antidiabetes function still needs further study. Gut microbiota disorder is one of the important factors that cause diabetes. To explore the effect of SL decoction on intestinal microbiota, gut microbiota of mice was analyzed by sequencing the gut bacterial 16S rRNA V3+V4 region and metagenomics. In this study, results demonstrated that SL decoction had a better hypoglycemic effect and β cell protection effect than either ginseng or Coptis chinensis. Alpha diversity analysis showed that all interventions with ginseng, Coptis, and SL decoction could reverse the increased diversity and richness of gut microbiota in db/db mice. PCoA analysis showed oral SL decoction significantly alters gut microbiota composition in db/db mice. 395 OTUs showed significant differences after SL treatment, of which 37 OTUs enriched by SL decoction showed a significant negative correlation with FBG, and 204 OTUs decreased by SL decoction showed a significant positive correlation with FBG. Results of KEGG analysis and metagenomic sequencing showed that SL decoction could reduce the Prevotellaceae, Rikenellaceae, and Helicobacteraceae, which were related to lipopolysaccharide biosynthesis, riboflavin metabolism, and peroxisome, respectively. It could also upregulate the abundance of Bacteroidaceae, which contributed to the metabolism of starch and sucrose as well as pentose-glucuronate interconversions. In the species level, SL decoction significantly upregulates the relative abundance of Bacteroides_acidifaciens which showed a significant negative correlation with FBG and was reported to be a potential agent for modulating metabolic disorders such as diabetes and obesity. In conclusion, SL decoction was effective in hypoglycemia and its mechanism may be related to regulating gut microbiota via upregulating Bacteroides_acidifaciens.http://dx.doi.org/10.1155/2022/7802107 |
| spellingShingle | Rui-xi Sun Wei-jun Huang Yao Xiao Dou-dou Wang Guo-hua Mu He Nan Bo-ran Ni Xiao-qiang Huang Hsuan-chuan Wang Yi-fan Liu Qiang Fu Jin-xi Zhao Shenlian (SL) Decoction, a Traditional Chinese Medicine Compound, May Ameliorate Blood Glucose via Mediating the Gut Microbiota in db/db Mice Journal of Diabetes Research |
| title | Shenlian (SL) Decoction, a Traditional Chinese Medicine Compound, May Ameliorate Blood Glucose via Mediating the Gut Microbiota in db/db Mice |
| title_full | Shenlian (SL) Decoction, a Traditional Chinese Medicine Compound, May Ameliorate Blood Glucose via Mediating the Gut Microbiota in db/db Mice |
| title_fullStr | Shenlian (SL) Decoction, a Traditional Chinese Medicine Compound, May Ameliorate Blood Glucose via Mediating the Gut Microbiota in db/db Mice |
| title_full_unstemmed | Shenlian (SL) Decoction, a Traditional Chinese Medicine Compound, May Ameliorate Blood Glucose via Mediating the Gut Microbiota in db/db Mice |
| title_short | Shenlian (SL) Decoction, a Traditional Chinese Medicine Compound, May Ameliorate Blood Glucose via Mediating the Gut Microbiota in db/db Mice |
| title_sort | shenlian sl decoction a traditional chinese medicine compound may ameliorate blood glucose via mediating the gut microbiota in db db mice |
| url | http://dx.doi.org/10.1155/2022/7802107 |
| work_keys_str_mv | AT ruixisun shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice AT weijunhuang shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice AT yaoxiao shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice AT doudouwang shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice AT guohuamu shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice AT henan shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice AT boranni shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice AT xiaoqianghuang shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice AT hsuanchuanwang shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice AT yifanliu shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice AT qiangfu shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice AT jinxizhao shenliansldecoctionatraditionalchinesemedicinecompoundmayamelioratebloodglucoseviamediatingthegutmicrobiotaindbdbmice |