SPP1 mRNA Expression Is Associated with M2 Macrophage Infiltration and Poor Prognosis in Triple-Negative Breast Cancer
(1): Triple-negative breast cancer (TNBC) is an especially aggressive form of breast cancer defined by a poor prognosis and a lack of effective treatment options. There is a pressing need for validated predictive and prognostic biomarkers to assist in making treatment decisions and improve the progn...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/46/12/806 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850050894535589888 |
|---|---|
| author | Yu-Chia Chen Chia-Ching Chen Rong-Fu Chen Hsin-Hung Chen Po-Ming Chen |
| author_facet | Yu-Chia Chen Chia-Ching Chen Rong-Fu Chen Hsin-Hung Chen Po-Ming Chen |
| author_sort | Yu-Chia Chen |
| collection | DOAJ |
| description | (1): Triple-negative breast cancer (TNBC) is an especially aggressive form of breast cancer defined by a poor prognosis and a lack of effective treatment options. There is a pressing need for validated predictive and prognostic biomarkers to assist in making treatment decisions and improve the prognostic accuracy for patients with this challenging disease. (2): We analyzed the RNA-seq data from three TNBC tissue samples alongside their corresponding normal tissues. Gene set enrichment analysis (GSEA) identified potential pathways. Additionally, we examined SPP1 mRNA expression datasets available in the Kaplan–Meier plotter and investigated the SPP1 protein expression patterns in our own tissue microarray cohort via immunohistochemistry. (3): The results revealed that genes associated with the Toll-like receptor signaling pathway showed a significant increase in activity in TNBC tissues when compared to normal breast tissues. Furthermore, <i>SPP1</i> expression was found to be elevated in the TCGA TNBC dataset and correlated with a poor prognosis. This pattern was corroborated at the protein level in our TNBC tissue cohort; however, SPP1 protein expression did not demonstrate a significant impact on survival. Notably, <i>SPP1</i> mRNA expression was strongly linked to tumor-associated macrophages (TAMs), particularly the M2 macrophage subtype, indicating a substantial association in the context of TNBC. (4): Our research highlights the significance of <i>SPP1</i> mRNA as a key prognostic indicator and a potential molecular responder for TNBC treatment utilizing targeted therapies that focus on Toll-like receptor signaling pathways. |
| format | Article |
| id | doaj-art-3f2dd826dec8470c9ac35cbec9f55504 |
| institution | DOAJ |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-3f2dd826dec8470c9ac35cbec9f555042025-08-20T02:53:19ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-11-014612134991351310.3390/cimb46120806SPP1 mRNA Expression Is Associated with M2 Macrophage Infiltration and Poor Prognosis in Triple-Negative Breast CancerYu-Chia Chen0Chia-Ching Chen1Rong-Fu Chen2Hsin-Hung Chen3Po-Ming Chen4Division of General Surgery, Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 813414, TaiwanDepartment of Emergency Medicine, Chang Bing Show Chwan Memorial Hospital, Changhua 505, TaiwanDivision of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung 807, TaiwanDepartment of Medical Education and Research, Kaohsiung Veterans General Hospital, No. 386, Dazhong 1st Road, Zuoying District, Kaohsiung 813414, TaiwanResearch Assistant Center, Show Chwan Memorial Hospital, No. 542, Section 1, Zhongshan Road, Changhua 500, Taiwan(1): Triple-negative breast cancer (TNBC) is an especially aggressive form of breast cancer defined by a poor prognosis and a lack of effective treatment options. There is a pressing need for validated predictive and prognostic biomarkers to assist in making treatment decisions and improve the prognostic accuracy for patients with this challenging disease. (2): We analyzed the RNA-seq data from three TNBC tissue samples alongside their corresponding normal tissues. Gene set enrichment analysis (GSEA) identified potential pathways. Additionally, we examined SPP1 mRNA expression datasets available in the Kaplan–Meier plotter and investigated the SPP1 protein expression patterns in our own tissue microarray cohort via immunohistochemistry. (3): The results revealed that genes associated with the Toll-like receptor signaling pathway showed a significant increase in activity in TNBC tissues when compared to normal breast tissues. Furthermore, <i>SPP1</i> expression was found to be elevated in the TCGA TNBC dataset and correlated with a poor prognosis. This pattern was corroborated at the protein level in our TNBC tissue cohort; however, SPP1 protein expression did not demonstrate a significant impact on survival. Notably, <i>SPP1</i> mRNA expression was strongly linked to tumor-associated macrophages (TAMs), particularly the M2 macrophage subtype, indicating a substantial association in the context of TNBC. (4): Our research highlights the significance of <i>SPP1</i> mRNA as a key prognostic indicator and a potential molecular responder for TNBC treatment utilizing targeted therapies that focus on Toll-like receptor signaling pathways.https://www.mdpi.com/1467-3045/46/12/806triple-negative breast cancerSPP1prognosis |
| spellingShingle | Yu-Chia Chen Chia-Ching Chen Rong-Fu Chen Hsin-Hung Chen Po-Ming Chen SPP1 mRNA Expression Is Associated with M2 Macrophage Infiltration and Poor Prognosis in Triple-Negative Breast Cancer Current Issues in Molecular Biology triple-negative breast cancer SPP1 prognosis |
| title | SPP1 mRNA Expression Is Associated with M2 Macrophage Infiltration and Poor Prognosis in Triple-Negative Breast Cancer |
| title_full | SPP1 mRNA Expression Is Associated with M2 Macrophage Infiltration and Poor Prognosis in Triple-Negative Breast Cancer |
| title_fullStr | SPP1 mRNA Expression Is Associated with M2 Macrophage Infiltration and Poor Prognosis in Triple-Negative Breast Cancer |
| title_full_unstemmed | SPP1 mRNA Expression Is Associated with M2 Macrophage Infiltration and Poor Prognosis in Triple-Negative Breast Cancer |
| title_short | SPP1 mRNA Expression Is Associated with M2 Macrophage Infiltration and Poor Prognosis in Triple-Negative Breast Cancer |
| title_sort | spp1 mrna expression is associated with m2 macrophage infiltration and poor prognosis in triple negative breast cancer |
| topic | triple-negative breast cancer SPP1 prognosis |
| url | https://www.mdpi.com/1467-3045/46/12/806 |
| work_keys_str_mv | AT yuchiachen spp1mrnaexpressionisassociatedwithm2macrophageinfiltrationandpoorprognosisintriplenegativebreastcancer AT chiachingchen spp1mrnaexpressionisassociatedwithm2macrophageinfiltrationandpoorprognosisintriplenegativebreastcancer AT rongfuchen spp1mrnaexpressionisassociatedwithm2macrophageinfiltrationandpoorprognosisintriplenegativebreastcancer AT hsinhungchen spp1mrnaexpressionisassociatedwithm2macrophageinfiltrationandpoorprognosisintriplenegativebreastcancer AT pomingchen spp1mrnaexpressionisassociatedwithm2macrophageinfiltrationandpoorprognosisintriplenegativebreastcancer |