Assessment of the Effect of Selenium Nanoparticles on Virulence Genes Expression csgD, adrA, and gcpA of Multi-Drug Resistant Salmonella enterica
This study explores the use of Selenium nanoparticles (SeNPs) to combat multi-drug resistant (MDR) Salmonella enterica serovars Typhi and Typhimurium, with a focus on their biofilm formation and antibiotic resistance patterns. The research involved isolating these Salmonella serovars from clinical s...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Anbar
2024-12-01
|
| Series: | مجلة جامعة الانبار للعلوم الصرفة |
| Subjects: | |
| Online Access: | https://juaps.uoanbar.edu.iq/article_185747_6002e0dc2096396c8f0ca62b4afd7821.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850194770615336960 |
|---|---|
| author | mariam Ayad Sawsan Al-Quhli Mahir Jassim |
| author_facet | mariam Ayad Sawsan Al-Quhli Mahir Jassim |
| author_sort | mariam Ayad |
| collection | DOAJ |
| description | This study explores the use of Selenium nanoparticles (SeNPs) to combat multi-drug resistant (MDR) Salmonella enterica serovars Typhi and Typhimurium, with a focus on their biofilm formation and antibiotic resistance patterns. The research involved isolating these Salmonella serovars from clinical samples, assessing their biofilm-forming abilities using the Microtiter plate assay, and analyzing their resistance to various antibiotics. A significant finding of this study is the high propensity of Salmonella isolates to form biofilms on plastic surfaces, raising concerns for infection control and food safety. The antibiotic susceptibility tests revealed an alarming level of multi-drug resistance, particularly in isolates from immunocompromised patients. This resistance was evident against frontline antibiotics like chloramphenicol, amoxicillin, and ceftriaxone. Notably, the study demonstrates that SeNPs, when combined with antibiotics, significantly improve their effectiveness. This enhancement is particularly evident in the reduced expression of biofilm- and virulence-related genes such as adrA, gcpA, and csgD. SeNPs could be a promising approach in treating resistant Salmonella infections, offering a potential solution to the escalating problem of antibiotic resistance.Overall, the study highlights the urgent need for alternative treatments against MDR Salmonella and suggests that SeNPs might be an effective option, warranting further research for clinical application. |
| format | Article |
| id | doaj-art-3f293a16cc5641c3b0a031f0ff0aa2b9 |
| institution | OA Journals |
| issn | 1991-8941 2706-6703 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة جامعة الانبار للعلوم الصرفة |
| spelling | doaj-art-3f293a16cc5641c3b0a031f0ff0aa2b92025-08-20T02:13:55ZengUniversity of Anbarمجلة جامعة الانبار للعلوم الصرفة1991-89412706-67032024-12-0118251510.37652/juaps.2024.146103.1178185747Assessment of the Effect of Selenium Nanoparticles on Virulence Genes Expression csgD, adrA, and gcpA of Multi-Drug Resistant Salmonella entericamariam Ayad0Sawsan Al-Quhli1Mahir Jassim2Department microbiology , College of Medicine, University of AnbarDepartment of Microbiology, College of Medicine, University of Anbar,Ramadi,Iraq,3 Internal Medicine Department, College of Medicine, University of AnbarThis study explores the use of Selenium nanoparticles (SeNPs) to combat multi-drug resistant (MDR) Salmonella enterica serovars Typhi and Typhimurium, with a focus on their biofilm formation and antibiotic resistance patterns. The research involved isolating these Salmonella serovars from clinical samples, assessing their biofilm-forming abilities using the Microtiter plate assay, and analyzing their resistance to various antibiotics. A significant finding of this study is the high propensity of Salmonella isolates to form biofilms on plastic surfaces, raising concerns for infection control and food safety. The antibiotic susceptibility tests revealed an alarming level of multi-drug resistance, particularly in isolates from immunocompromised patients. This resistance was evident against frontline antibiotics like chloramphenicol, amoxicillin, and ceftriaxone. Notably, the study demonstrates that SeNPs, when combined with antibiotics, significantly improve their effectiveness. This enhancement is particularly evident in the reduced expression of biofilm- and virulence-related genes such as adrA, gcpA, and csgD. SeNPs could be a promising approach in treating resistant Salmonella infections, offering a potential solution to the escalating problem of antibiotic resistance.Overall, the study highlights the urgent need for alternative treatments against MDR Salmonella and suggests that SeNPs might be an effective option, warranting further research for clinical application.https://juaps.uoanbar.edu.iq/article_185747_6002e0dc2096396c8f0ca62b4afd7821.pdfsalmonellagene expresionantibiotic resistance |
| spellingShingle | mariam Ayad Sawsan Al-Quhli Mahir Jassim Assessment of the Effect of Selenium Nanoparticles on Virulence Genes Expression csgD, adrA, and gcpA of Multi-Drug Resistant Salmonella enterica مجلة جامعة الانبار للعلوم الصرفة salmonella gene expresion antibiotic resistance |
| title | Assessment of the Effect of Selenium Nanoparticles on Virulence Genes Expression csgD, adrA, and gcpA of Multi-Drug Resistant Salmonella enterica |
| title_full | Assessment of the Effect of Selenium Nanoparticles on Virulence Genes Expression csgD, adrA, and gcpA of Multi-Drug Resistant Salmonella enterica |
| title_fullStr | Assessment of the Effect of Selenium Nanoparticles on Virulence Genes Expression csgD, adrA, and gcpA of Multi-Drug Resistant Salmonella enterica |
| title_full_unstemmed | Assessment of the Effect of Selenium Nanoparticles on Virulence Genes Expression csgD, adrA, and gcpA of Multi-Drug Resistant Salmonella enterica |
| title_short | Assessment of the Effect of Selenium Nanoparticles on Virulence Genes Expression csgD, adrA, and gcpA of Multi-Drug Resistant Salmonella enterica |
| title_sort | assessment of the effect of selenium nanoparticles on virulence genes expression csgd adra and gcpa of multi drug resistant salmonella enterica |
| topic | salmonella gene expresion antibiotic resistance |
| url | https://juaps.uoanbar.edu.iq/article_185747_6002e0dc2096396c8f0ca62b4afd7821.pdf |
| work_keys_str_mv | AT mariamayad assessmentoftheeffectofseleniumnanoparticlesonvirulencegenesexpressioncsgdadraandgcpaofmultidrugresistantsalmonellaenterica AT sawsanalquhli assessmentoftheeffectofseleniumnanoparticlesonvirulencegenesexpressioncsgdadraandgcpaofmultidrugresistantsalmonellaenterica AT mahirjassim assessmentoftheeffectofseleniumnanoparticlesonvirulencegenesexpressioncsgdadraandgcpaofmultidrugresistantsalmonellaenterica |