ASD-GFP Vectors for In Vivo Expression Technology in Pseudomonas aeruginosa and Other Gram-Negative Bacteria
We describe the construction of promoter probe vectors designed for identification of bacterial genes induced in vitro and/or in vivo and for measurement of gene expression levels for in vivo expression technology. These plasmids use the Pseudomonas aeruginosa aspartate β-semialdehyde dehydrogenase...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-02-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98242st02 |
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| Summary: | We describe the construction of promoter probe vectors designed for identification of bacterial genes induced in vitro and/or in vivo and for measurement of gene expression levels for in vivo expression technology. These plasmids use the Pseudomonas aeruginosa aspartate β-semialdehyde dehydrogenase (asd) gene as a selectable marker and β-galactosidase (pIVPRO, 10.88 kb) or mutant green fluorescent protein with enhanced fluorescence properties (mut3GFP, pIVET-GFP, 5.48 kb) as reporter gene systems. The proposed strategies can be adapted for use in most Gram-negative bacteria. |
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| ISSN: | 0736-6205 1940-9818 |