The bantam microRNA is associated with drosophila fragile X mental retardation protein and regulates the fate of germline stem cells.
Fragile X syndrome, a common form of inherited mental retardation, is caused by the loss of fragile X mental retardation protein (FMRP). We have previously demonstrated that dFmr1, the Drosophila ortholog of the fragile X mental retardation 1 gene, plays a role in the proper maintenance of germline...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2009-04-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1000444&type=printable |
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| _version_ | 1849686627408936960 |
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| author | Yingyue Yang Shunliang Xu Laixin Xia Jun Wang Shengmei Wen Peng Jin Dahua Chen |
| author_facet | Yingyue Yang Shunliang Xu Laixin Xia Jun Wang Shengmei Wen Peng Jin Dahua Chen |
| author_sort | Yingyue Yang |
| collection | DOAJ |
| description | Fragile X syndrome, a common form of inherited mental retardation, is caused by the loss of fragile X mental retardation protein (FMRP). We have previously demonstrated that dFmr1, the Drosophila ortholog of the fragile X mental retardation 1 gene, plays a role in the proper maintenance of germline stem cells in Drosophila ovary; however, the molecular mechanism behind this remains elusive. In this study, we used an immunoprecipitation assay to reveal that specific microRNAs (miRNAs), particularly the bantam miRNA (bantam), are physically associated with dFmrp in ovary. We show that, like dFmr1, bantam is not only required for repressing primordial germ cell differentiation, it also functions as an extrinsic factor for germline stem cell maintenance. Furthermore, we find that bantam genetically interacts with dFmr1 to regulate the fate of germline stem cells. Collectively, our results support the notion that the FMRP-mediated translation pathway functions through specific miRNAs to control stem cell regulation. |
| format | Article |
| id | doaj-art-3f2245d06be54805b01730319bc1064e |
| institution | DOAJ |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2009-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-3f2245d06be54805b01730319bc1064e2025-08-20T03:22:38ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042009-04-0154e100044410.1371/journal.pgen.1000444The bantam microRNA is associated with drosophila fragile X mental retardation protein and regulates the fate of germline stem cells.Yingyue YangShunliang XuLaixin XiaJun WangShengmei WenPeng JinDahua ChenFragile X syndrome, a common form of inherited mental retardation, is caused by the loss of fragile X mental retardation protein (FMRP). We have previously demonstrated that dFmr1, the Drosophila ortholog of the fragile X mental retardation 1 gene, plays a role in the proper maintenance of germline stem cells in Drosophila ovary; however, the molecular mechanism behind this remains elusive. In this study, we used an immunoprecipitation assay to reveal that specific microRNAs (miRNAs), particularly the bantam miRNA (bantam), are physically associated with dFmrp in ovary. We show that, like dFmr1, bantam is not only required for repressing primordial germ cell differentiation, it also functions as an extrinsic factor for germline stem cell maintenance. Furthermore, we find that bantam genetically interacts with dFmr1 to regulate the fate of germline stem cells. Collectively, our results support the notion that the FMRP-mediated translation pathway functions through specific miRNAs to control stem cell regulation.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1000444&type=printable |
| spellingShingle | Yingyue Yang Shunliang Xu Laixin Xia Jun Wang Shengmei Wen Peng Jin Dahua Chen The bantam microRNA is associated with drosophila fragile X mental retardation protein and regulates the fate of germline stem cells. PLoS Genetics |
| title | The bantam microRNA is associated with drosophila fragile X mental retardation protein and regulates the fate of germline stem cells. |
| title_full | The bantam microRNA is associated with drosophila fragile X mental retardation protein and regulates the fate of germline stem cells. |
| title_fullStr | The bantam microRNA is associated with drosophila fragile X mental retardation protein and regulates the fate of germline stem cells. |
| title_full_unstemmed | The bantam microRNA is associated with drosophila fragile X mental retardation protein and regulates the fate of germline stem cells. |
| title_short | The bantam microRNA is associated with drosophila fragile X mental retardation protein and regulates the fate of germline stem cells. |
| title_sort | bantam microrna is associated with drosophila fragile x mental retardation protein and regulates the fate of germline stem cells |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1000444&type=printable |
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