Circulating tsRNAs serve as potential biomarkers for predicting postoperative delirium in elderly patients receiving lower extremity orthopedic surgery
BackgroundPostoperative delirium (POD) is a serious neuropsychiatric complication in elderly surgical patients, yet its pathogenesis remains incompletely understood. Transfer RNA-derived small RNAs (tsRNAs) have emerged as crucial regulators in neurological disorders. We investigated whether specifi...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Psychiatry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpsyt.2025.1522984/full |
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| author | Angyang Cao Angyang Cao Rui Zhao Rui Zhao Chunqu Chen Chunqu Chen Can Wu Can Wu Yiwei Zhang Yiwei Zhang Changshun Huang Changshun Huang Binbin Zhu Binbin Zhu |
| author_facet | Angyang Cao Angyang Cao Rui Zhao Rui Zhao Chunqu Chen Chunqu Chen Can Wu Can Wu Yiwei Zhang Yiwei Zhang Changshun Huang Changshun Huang Binbin Zhu Binbin Zhu |
| author_sort | Angyang Cao |
| collection | DOAJ |
| description | BackgroundPostoperative delirium (POD) is a serious neuropsychiatric complication in elderly surgical patients, yet its pathogenesis remains incompletely understood. Transfer RNA-derived small RNAs (tsRNAs) have emerged as crucial regulators in neurological disorders. We investigated whether specific tsRNAs could serve as predictive biomarkers for POD.MethodsThis study conducted a prospective case-control study of 158 elderly patients (≥60 years) undergoing orthopedic surgery. Plasma samples were collected preoperatively and on postoperative day 3.tsRNA expression profiles were analyzed using RNA sequencing and validated by RT-qPCR. Propensity score matching was performed to balance demographic and clinical variables. The predictive value of candidate tsRNAs was assessed using ROC analysis, and their potential functions were explored through bioinformatic analyses.ResultsAmong 128 non-POD and 30 POD patients, two tsRNAs (Other-14: 31-tRNA-Gly-CCC-3 and Other-39: 73-tRNA-Arg-TCG-5) showed significantly elevated preoperative levels in POD patients (p<0.001).ROC analysis revealed strong predictive performance (AUC=0.868 and 0.956, respectively).These differences persisted in the propensity-matched cohort (29 pairs).Bioinformatic analyses indicated enrichment in pathways related to neurotransmission, inflammation, and metabolism.ConclusionThis study identified novel tsRNA biomarkers that robustly predict POD risk and provide insights into its molecular pathogenesis. These findings may facilitate early risk stratification and preventive interventions. |
| format | Article |
| id | doaj-art-3f1c1edbbafd47b69e718868bb26fdb3 |
| institution | Kabale University |
| issn | 1664-0640 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Psychiatry |
| spelling | doaj-art-3f1c1edbbafd47b69e718868bb26fdb32025-08-20T03:40:41ZengFrontiers Media S.A.Frontiers in Psychiatry1664-06402025-03-011610.3389/fpsyt.2025.15229841522984Circulating tsRNAs serve as potential biomarkers for predicting postoperative delirium in elderly patients receiving lower extremity orthopedic surgeryAngyang Cao0Angyang Cao1Rui Zhao2Rui Zhao3Chunqu Chen4Chunqu Chen5Can Wu6Can Wu7Yiwei Zhang8Yiwei Zhang9Changshun Huang10Changshun Huang11Binbin Zhu12Binbin Zhu13Department of Anesthesiology, The First Affliated Hospital of Ningbo University, Ningbo, ChinaSchool of Medicine, Ningbo University, Ningbo, ChinaDepartment of Anesthesiology, The First Affliated Hospital of Ningbo University, Ningbo, ChinaSchool of Medicine, Ningbo University, Ningbo, ChinaSchool of Medicine, Ningbo University, Ningbo, ChinaDepartment of imaging, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, ChinaSchool of Medicine, Ningbo University, Ningbo, ChinaDepartment of Clinical laboratory, Jinhua Maternal and Child Health Care Hospital, Jinhua, ChinaDepartment of Anesthesiology, The First Affliated Hospital of Ningbo University, Ningbo, ChinaSchool of Medicine, Ningbo University, Ningbo, ChinaDepartment of Anesthesiology, The First Affliated Hospital of Ningbo University, Ningbo, ChinaSchool of Medicine, Ningbo University, Ningbo, ChinaDepartment of Anesthesiology, The First Affliated Hospital of Ningbo University, Ningbo, ChinaSchool of Medicine, Ningbo University, Ningbo, ChinaBackgroundPostoperative delirium (POD) is a serious neuropsychiatric complication in elderly surgical patients, yet its pathogenesis remains incompletely understood. Transfer RNA-derived small RNAs (tsRNAs) have emerged as crucial regulators in neurological disorders. We investigated whether specific tsRNAs could serve as predictive biomarkers for POD.MethodsThis study conducted a prospective case-control study of 158 elderly patients (≥60 years) undergoing orthopedic surgery. Plasma samples were collected preoperatively and on postoperative day 3.tsRNA expression profiles were analyzed using RNA sequencing and validated by RT-qPCR. Propensity score matching was performed to balance demographic and clinical variables. The predictive value of candidate tsRNAs was assessed using ROC analysis, and their potential functions were explored through bioinformatic analyses.ResultsAmong 128 non-POD and 30 POD patients, two tsRNAs (Other-14: 31-tRNA-Gly-CCC-3 and Other-39: 73-tRNA-Arg-TCG-5) showed significantly elevated preoperative levels in POD patients (p<0.001).ROC analysis revealed strong predictive performance (AUC=0.868 and 0.956, respectively).These differences persisted in the propensity-matched cohort (29 pairs).Bioinformatic analyses indicated enrichment in pathways related to neurotransmission, inflammation, and metabolism.ConclusionThis study identified novel tsRNA biomarkers that robustly predict POD risk and provide insights into its molecular pathogenesis. These findings may facilitate early risk stratification and preventive interventions.https://www.frontiersin.org/articles/10.3389/fpsyt.2025.1522984/fulltsRNApostoperative delirium (POD)RT-qPCRelderly patientspropensity score matching (PSM) |
| spellingShingle | Angyang Cao Angyang Cao Rui Zhao Rui Zhao Chunqu Chen Chunqu Chen Can Wu Can Wu Yiwei Zhang Yiwei Zhang Changshun Huang Changshun Huang Binbin Zhu Binbin Zhu Circulating tsRNAs serve as potential biomarkers for predicting postoperative delirium in elderly patients receiving lower extremity orthopedic surgery Frontiers in Psychiatry tsRNA postoperative delirium (POD) RT-qPCR elderly patients propensity score matching (PSM) |
| title | Circulating tsRNAs serve as potential biomarkers for predicting postoperative delirium in elderly patients receiving lower extremity orthopedic surgery |
| title_full | Circulating tsRNAs serve as potential biomarkers for predicting postoperative delirium in elderly patients receiving lower extremity orthopedic surgery |
| title_fullStr | Circulating tsRNAs serve as potential biomarkers for predicting postoperative delirium in elderly patients receiving lower extremity orthopedic surgery |
| title_full_unstemmed | Circulating tsRNAs serve as potential biomarkers for predicting postoperative delirium in elderly patients receiving lower extremity orthopedic surgery |
| title_short | Circulating tsRNAs serve as potential biomarkers for predicting postoperative delirium in elderly patients receiving lower extremity orthopedic surgery |
| title_sort | circulating tsrnas serve as potential biomarkers for predicting postoperative delirium in elderly patients receiving lower extremity orthopedic surgery |
| topic | tsRNA postoperative delirium (POD) RT-qPCR elderly patients propensity score matching (PSM) |
| url | https://www.frontiersin.org/articles/10.3389/fpsyt.2025.1522984/full |
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