Protocol for isolation of endothelial cells from adult mouse brain

Protocol for isolation of endothelial cells from adult mouse brain

Summary: Brain endothelial cells (ECs) line blood vessel walls and form the blood-brain barrier (BBB), facilitating interactions between the brain and the periphery. Here, we present a protocol for isolating purified ECs from adult mouse brains using fluorescence-activated cell sorting (FACS) for do...

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Bibliographic Details
Main Authors: Audrey G. Miglietta, Jennifer David-Bercholz, Roeben N. Munji, Robert S. Pulido, Allison L. Soung, Niccolò Terrando, Richard Daneman, Ting Yang
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Cell isolation
Cell separation/fractionation
Flow Cytometry
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002436
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