In Vitro Biological and GC-MS Analysis of Whole Plant Calotropis procera
Calotropis procera is a medicinal, traditional, and therapeutic plant in Pakistan. In this research study, the biological activities, for instance, anticancer, antileishmanial, antibacterial, antifungal, anti-inflammatory, and brine shrimp lethality assay and GC-MS are studied. MTT assay, antileishm...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2024/2012983 |
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| author | Qahir Shah Jahangir Khan Achakzai Muhammad Anwar Panezai Basira Akhtar Abdul Manan Kakar Ali Akbar Shahabuddin Kakar Nisar Ahmed Shahwani Javed Khan Nazima Yousaf Khan Ghulam Mustafa Khan Nizam Baloch Bakht Zareen Rahim Noor Hassan |
| author_facet | Qahir Shah Jahangir Khan Achakzai Muhammad Anwar Panezai Basira Akhtar Abdul Manan Kakar Ali Akbar Shahabuddin Kakar Nisar Ahmed Shahwani Javed Khan Nazima Yousaf Khan Ghulam Mustafa Khan Nizam Baloch Bakht Zareen Rahim Noor Hassan |
| author_sort | Qahir Shah |
| collection | DOAJ |
| description | Calotropis procera is a medicinal, traditional, and therapeutic plant in Pakistan. In this research study, the biological activities, for instance, anticancer, antileishmanial, antibacterial, antifungal, anti-inflammatory, and brine shrimp lethality assay and GC-MS are studied. MTT assay, antileishmanial bioassay, microplate Alamar Blue assay, agar tube dilution method, oxidative burst assay using chemiluminescence technique, B-hatching techniques, and triple quadrupole acquisition method MS parameters were the methods used for anticancer, antileishmanial, antibacterial, antifungal, anti-inflammatory, brine shrimp lethality assay, and gas chromatography-mass spectrometry. Whole plant methanol extract of Calotropis procera (WMECP) inhibited 69.1% of the growth of HeLa cell line with an IC50 value of 3.1 ± 0.4 and whole plant n-hexane fraction of Calotropis procera (WHFCP) and whole plant and aqueous fraction of Calotropis procera (WAFCP) inhibited the growth by 70.2% and 65.2% with IC50 values of 5.0 ± 0.3 and 17.1 ± 1.0. Whole plant methanol extract of Calotropis procera (WMECP) inhibited 70.1% of the growth of the PC3 cell line with an IC50 value of 5.1 ± 0.3 and whole plant n-hexane fraction of Calotropis procera (WHFCP) and whole plant aqueous fraction of Calotropis procera (WAFCP) inhibited 61.6% and 59.7% with IC50 values of 3.7 ± 0.5 and 16.4 ± 1.0. None of the extract and fractions of Calotropis procera showed anticancer activities against the 3T3 cell line. None of the extract and fractions of Calotropis procera showed antileishmanial, antibacterial, antifungal, and anti-inflammatory activities. Whole plant methanol extract of Calotropis procera (WMECP) exhibited lethality at the highest concentration while other fractions did not exhibit lethality. GC-MS studies revealed that the whole plant methanol extract of Calotropis procera (WMECP) consists of 11 compounds, whole plant n-hexane fraction of Calotropis procera (WHFCP) consists of 9 compounds, and whole plant aqueous fraction of Calotropis procera (WAFCP) consists of 7 compounds. |
| format | Article |
| id | doaj-art-3f02d747a2c04cba82b973569bef74ae |
| institution | Kabale University |
| issn | 2090-9071 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
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| series | Journal of Chemistry |
| spelling | doaj-art-3f02d747a2c04cba82b973569bef74ae2025-02-03T07:23:37ZengWileyJournal of Chemistry2090-90712024-01-01202410.1155/2024/2012983In Vitro Biological and GC-MS Analysis of Whole Plant Calotropis proceraQahir Shah0Jahangir Khan Achakzai1Muhammad Anwar Panezai2Basira Akhtar3Abdul Manan Kakar4Ali Akbar5Shahabuddin Kakar6Nisar Ahmed Shahwani7Javed Khan8Nazima Yousaf Khan9Ghulam Mustafa Khan10Nizam Baloch11Bakht Zareen Rahim12Noor Hassan13Department of ChemistryDiscipline of BiochemistryInstitute of BiochemistryDepartment of BotanyInstitute of BiochemistryCentre for Biotechnology and Microbiology (CBM) University of SwatDepartment of ZoologyFaculty of PharmacyDepartment of MicrobiologyInstitute of BiochemistryDepartment of ChemistryDepartment of ChemistryDepartment of BotanyInstitute of BiochemistryCalotropis procera is a medicinal, traditional, and therapeutic plant in Pakistan. In this research study, the biological activities, for instance, anticancer, antileishmanial, antibacterial, antifungal, anti-inflammatory, and brine shrimp lethality assay and GC-MS are studied. MTT assay, antileishmanial bioassay, microplate Alamar Blue assay, agar tube dilution method, oxidative burst assay using chemiluminescence technique, B-hatching techniques, and triple quadrupole acquisition method MS parameters were the methods used for anticancer, antileishmanial, antibacterial, antifungal, anti-inflammatory, brine shrimp lethality assay, and gas chromatography-mass spectrometry. Whole plant methanol extract of Calotropis procera (WMECP) inhibited 69.1% of the growth of HeLa cell line with an IC50 value of 3.1 ± 0.4 and whole plant n-hexane fraction of Calotropis procera (WHFCP) and whole plant and aqueous fraction of Calotropis procera (WAFCP) inhibited the growth by 70.2% and 65.2% with IC50 values of 5.0 ± 0.3 and 17.1 ± 1.0. Whole plant methanol extract of Calotropis procera (WMECP) inhibited 70.1% of the growth of the PC3 cell line with an IC50 value of 5.1 ± 0.3 and whole plant n-hexane fraction of Calotropis procera (WHFCP) and whole plant aqueous fraction of Calotropis procera (WAFCP) inhibited 61.6% and 59.7% with IC50 values of 3.7 ± 0.5 and 16.4 ± 1.0. None of the extract and fractions of Calotropis procera showed anticancer activities against the 3T3 cell line. None of the extract and fractions of Calotropis procera showed antileishmanial, antibacterial, antifungal, and anti-inflammatory activities. Whole plant methanol extract of Calotropis procera (WMECP) exhibited lethality at the highest concentration while other fractions did not exhibit lethality. GC-MS studies revealed that the whole plant methanol extract of Calotropis procera (WMECP) consists of 11 compounds, whole plant n-hexane fraction of Calotropis procera (WHFCP) consists of 9 compounds, and whole plant aqueous fraction of Calotropis procera (WAFCP) consists of 7 compounds.http://dx.doi.org/10.1155/2024/2012983 |
| spellingShingle | Qahir Shah Jahangir Khan Achakzai Muhammad Anwar Panezai Basira Akhtar Abdul Manan Kakar Ali Akbar Shahabuddin Kakar Nisar Ahmed Shahwani Javed Khan Nazima Yousaf Khan Ghulam Mustafa Khan Nizam Baloch Bakht Zareen Rahim Noor Hassan In Vitro Biological and GC-MS Analysis of Whole Plant Calotropis procera Journal of Chemistry |
| title | In Vitro Biological and GC-MS Analysis of Whole Plant Calotropis procera |
| title_full | In Vitro Biological and GC-MS Analysis of Whole Plant Calotropis procera |
| title_fullStr | In Vitro Biological and GC-MS Analysis of Whole Plant Calotropis procera |
| title_full_unstemmed | In Vitro Biological and GC-MS Analysis of Whole Plant Calotropis procera |
| title_short | In Vitro Biological and GC-MS Analysis of Whole Plant Calotropis procera |
| title_sort | in vitro biological and gc ms analysis of whole plant calotropis procera |
| url | http://dx.doi.org/10.1155/2024/2012983 |
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