ToF-SIMS spectral analysis of Shewanella oneidensis MR-1 biofilms
Analysis of bacterial biofilms is particularly challenging and important with diverse applications from systems biology to biotechnology. Among the variety of techniques that have been applied, time-of-flight secondary ion mass spectrometry (ToF-SIMS) has many powerful features in studying the surfa...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Results in Surfaces and Interfaces |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666845924002162 |
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| author | Gabriel D. Parker Andrew Plymale Luke Hanley Xiao-Ying Yu |
| author_facet | Gabriel D. Parker Andrew Plymale Luke Hanley Xiao-Ying Yu |
| author_sort | Gabriel D. Parker |
| collection | DOAJ |
| description | Analysis of bacterial biofilms is particularly challenging and important with diverse applications from systems biology to biotechnology. Among the variety of techniques that have been applied, time-of-flight secondary ion mass spectrometry (ToF-SIMS) has many powerful features in studying the surface characteristics of biofilms. ToF-SIMS offers high spatial resolution, mass resolution, and mass accuracy, which permit surface sensitive analysis of biofilm components. Thus, ToF-SIMS provides a powerful solution to addressing the challenge of bacterial biofilm analysis. This dataset covers ToF-SIMS analysis of Shewanella oneidensis MR-1 isolated from freshwater lake sediment in New York state. The MR-1 strain is known to have metal and sulfur reducing properties and it can be used for bioremediation and wastewater treatment. There is a current need to identify small molecules and fragments produced from bacterial biofilms, especially those from extracellular polymeric substance (EPS). Static ToF-SIMS spectra of MR-1 were obtained using an IONTOF TOF.SIMS V instrument equipped with a 25 keV Bi3+ metal ion gun. Identified molecules and molecular fragments are compared against known biological databases and the reported peaks have at least 65 ppm mass accuracy. These molecules range from lipids, fatty acids, flavonoids, and quinolones to other naturally occurring organic compounds. It is anticipated that the mass spectral identification of key peaks will assist detection of metabolites, EPS molecules like polysaccharides, and biologically relevant small organic molecules using ToF-SIMS in future surface and interface research. |
| format | Article |
| id | doaj-art-3f004205dd4a498da0c527c97eddf5be |
| institution | DOAJ |
| issn | 2666-8459 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Surfaces and Interfaces |
| spelling | doaj-art-3f004205dd4a498da0c527c97eddf5be2025-08-20T02:57:33ZengElsevierResults in Surfaces and Interfaces2666-84592025-01-011810039610.1016/j.rsurfi.2024.100396ToF-SIMS spectral analysis of Shewanella oneidensis MR-1 biofilmsGabriel D. Parker0Andrew Plymale1Luke Hanley2Xiao-Ying Yu3Department of Chemistry, University of Illinois Chicago, Chicago, IL, 60607, USA; Materials Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37380, USAEnergy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99354, USADepartment of Chemistry, University of Illinois Chicago, Chicago, IL, 60607, USAMaterials Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37380, USA; Corresponding author.Analysis of bacterial biofilms is particularly challenging and important with diverse applications from systems biology to biotechnology. Among the variety of techniques that have been applied, time-of-flight secondary ion mass spectrometry (ToF-SIMS) has many powerful features in studying the surface characteristics of biofilms. ToF-SIMS offers high spatial resolution, mass resolution, and mass accuracy, which permit surface sensitive analysis of biofilm components. Thus, ToF-SIMS provides a powerful solution to addressing the challenge of bacterial biofilm analysis. This dataset covers ToF-SIMS analysis of Shewanella oneidensis MR-1 isolated from freshwater lake sediment in New York state. The MR-1 strain is known to have metal and sulfur reducing properties and it can be used for bioremediation and wastewater treatment. There is a current need to identify small molecules and fragments produced from bacterial biofilms, especially those from extracellular polymeric substance (EPS). Static ToF-SIMS spectra of MR-1 were obtained using an IONTOF TOF.SIMS V instrument equipped with a 25 keV Bi3+ metal ion gun. Identified molecules and molecular fragments are compared against known biological databases and the reported peaks have at least 65 ppm mass accuracy. These molecules range from lipids, fatty acids, flavonoids, and quinolones to other naturally occurring organic compounds. It is anticipated that the mass spectral identification of key peaks will assist detection of metabolites, EPS molecules like polysaccharides, and biologically relevant small organic molecules using ToF-SIMS in future surface and interface research.http://www.sciencedirect.com/science/article/pii/S2666845924002162Time-of-flight secondary ion mass spectrometryShewanella oneidensis MR-1BiofilmsMetabolitesFatty acidsextracellular polymeric substance |
| spellingShingle | Gabriel D. Parker Andrew Plymale Luke Hanley Xiao-Ying Yu ToF-SIMS spectral analysis of Shewanella oneidensis MR-1 biofilms Results in Surfaces and Interfaces Time-of-flight secondary ion mass spectrometry Shewanella oneidensis MR-1 Biofilms Metabolites Fatty acids extracellular polymeric substance |
| title | ToF-SIMS spectral analysis of Shewanella oneidensis MR-1 biofilms |
| title_full | ToF-SIMS spectral analysis of Shewanella oneidensis MR-1 biofilms |
| title_fullStr | ToF-SIMS spectral analysis of Shewanella oneidensis MR-1 biofilms |
| title_full_unstemmed | ToF-SIMS spectral analysis of Shewanella oneidensis MR-1 biofilms |
| title_short | ToF-SIMS spectral analysis of Shewanella oneidensis MR-1 biofilms |
| title_sort | tof sims spectral analysis of shewanella oneidensis mr 1 biofilms |
| topic | Time-of-flight secondary ion mass spectrometry Shewanella oneidensis MR-1 Biofilms Metabolites Fatty acids extracellular polymeric substance |
| url | http://www.sciencedirect.com/science/article/pii/S2666845924002162 |
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