CRISPR/Cas Technology in Insect Insecticide Resistance
Chemicals and biological insecticides play a crucial role as pest management strategies in modern agriculture and forestry. However, their excessive and unreasonable use inevitably leads to varying degrees of resistance among insect populations, which seriously affects the sustainability of insectic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/16/4/345 |
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| author | Qiuchen Xu Mingyun Wang Jiahui Zeng Hangzhen Sun Xiaoqi Wei Hui Jiang Xuping Shentu Dan Sun |
| author_facet | Qiuchen Xu Mingyun Wang Jiahui Zeng Hangzhen Sun Xiaoqi Wei Hui Jiang Xuping Shentu Dan Sun |
| author_sort | Qiuchen Xu |
| collection | DOAJ |
| description | Chemicals and biological insecticides play a crucial role as pest management strategies in modern agriculture and forestry. However, their excessive and unreasonable use inevitably leads to varying degrees of resistance among insect populations, which seriously affects the sustainability of insecticide use. One primary reason for this resistance is alterations or mutations in insect gene expression. One class of genes encodes proteins that serve as critical targets for insecticides to exert their toxic effects in insects, while another class of genes encodes proteins involved in the detoxification process of insecticides within insects. Reverse genetics has become a vital research tool for studying the molecular mechanisms underlying changes and mutations in these target genes and their impact on insect resistance. The advent of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the CRISPR-associated gene Cas as gene-editing technologies has significantly advanced our understanding of how insects adapt to and resist insecticides. This article aims to provide a comprehensive and objective review of the progress made using the CRISPR/Cas system in various arthropods within the field of pest control. |
| format | Article |
| id | doaj-art-3eef5f8eb01b41b391643884bed7de82 |
| institution | DOAJ |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-3eef5f8eb01b41b391643884bed7de822025-08-20T03:13:55ZengMDPI AGInsects2075-44502025-03-0116434510.3390/insects16040345CRISPR/Cas Technology in Insect Insecticide ResistanceQiuchen Xu0Mingyun Wang1Jiahui Zeng2Hangzhen Sun3Xiaoqi Wei4Hui Jiang5Xuping Shentu6Dan Sun7Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, ChinaKey Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, ChinaChemicals and biological insecticides play a crucial role as pest management strategies in modern agriculture and forestry. However, their excessive and unreasonable use inevitably leads to varying degrees of resistance among insect populations, which seriously affects the sustainability of insecticide use. One primary reason for this resistance is alterations or mutations in insect gene expression. One class of genes encodes proteins that serve as critical targets for insecticides to exert their toxic effects in insects, while another class of genes encodes proteins involved in the detoxification process of insecticides within insects. Reverse genetics has become a vital research tool for studying the molecular mechanisms underlying changes and mutations in these target genes and their impact on insect resistance. The advent of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the CRISPR-associated gene Cas as gene-editing technologies has significantly advanced our understanding of how insects adapt to and resist insecticides. This article aims to provide a comprehensive and objective review of the progress made using the CRISPR/Cas system in various arthropods within the field of pest control.https://www.mdpi.com/2075-4450/16/4/345insectsinsecticide resistanceCRISPR/Castarget site |
| spellingShingle | Qiuchen Xu Mingyun Wang Jiahui Zeng Hangzhen Sun Xiaoqi Wei Hui Jiang Xuping Shentu Dan Sun CRISPR/Cas Technology in Insect Insecticide Resistance Insects insects insecticide resistance CRISPR/Cas target site |
| title | CRISPR/Cas Technology in Insect Insecticide Resistance |
| title_full | CRISPR/Cas Technology in Insect Insecticide Resistance |
| title_fullStr | CRISPR/Cas Technology in Insect Insecticide Resistance |
| title_full_unstemmed | CRISPR/Cas Technology in Insect Insecticide Resistance |
| title_short | CRISPR/Cas Technology in Insect Insecticide Resistance |
| title_sort | crispr cas technology in insect insecticide resistance |
| topic | insects insecticide resistance CRISPR/Cas target site |
| url | https://www.mdpi.com/2075-4450/16/4/345 |
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