Liquid chromatography–mass spectrometry phytochemical analysis, antioxidant, anti–inflammatory and analgesic efficacy of Origanum majorana L. aerial part extracts collected from North–Eastern of Algeria
Origanum majorana L. (Lamiaceae) has been prescribed in folk medicine for therapeutic purposes such as cancer, asthma, dizziness, rheumatism and inflammatory diseases. To evaluate for the first time the decoction (DecE) and hydro–ethanolic (HEE) extracts of the aerial part of O. majorana. The phyto...
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Universidad del Zulia
2025-06-01
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| Series: | Revista Científica |
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| Online Access: | https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43929 |
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| author | Ahlem Karbab Noureddine Charef Salima Amari Areej Jaber Ayat Siedat Alaa Sanabrah Solomon Derese |
| author_facet | Ahlem Karbab Noureddine Charef Salima Amari Areej Jaber Ayat Siedat Alaa Sanabrah Solomon Derese |
| author_sort | Ahlem Karbab |
| collection | DOAJ |
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Origanum majorana L. (Lamiaceae) has been prescribed in folk medicine for therapeutic purposes such as cancer, asthma, dizziness, rheumatism and inflammatory diseases. To evaluate for the first time the decoction (DecE) and hydro–ethanolic (HEE) extracts of the aerial part of O. majorana. The phytochemical analysis (LC–MS/MS), antioxidant, anti–inflammatory and analgesic potentials were analysed. LC–MS/MS analysis identified 17 components in the extracts. The main compounds detected in the aerial parts of O. majorana include rosmarinic acid (≥ 50%), elagic acid (≥ 20%), indomethacin along with flavonoids, phenolics, benzopyrone, hydroquinones, indolyl acetic acid. Both extracts showed potent antioxidant effects in various antioxidant assay models. Through the eggs protein denaturation assay, both plant extracts showed high in vitro anti–inflammatory effect of the order of 86.91±0.00% for DecE and 85.04±0.00 % for HEE (1.4 mg·mL-1). Acute toxicity measurement revealed no mortality or behavioral changes during the testing period, confirming the two extracts not toxic with an LD50 > 2 g·kg-1. The in vivo anti–inflammatory effects were found to be statistically significant (P<0.05), reducing the edematous response by 78.40 ± 2.52 % and 77.36 ± 3.88 % in DecE and HEE, respectively. Additionally, at a dose of 400.0 mg·kg-1, both extracts exhibited significant analgesics activity (P<0.05) against acetic acid induced abdominal constriction in mice, with inhibition rates of 78.54 ± 3.30% for DecE and 66.99 ± 1.34% for HEE. The results indicate aerial part extracts of O. majorana have potent anti–inflammatory effects and may prove to have potential health benefits.
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| format | Article |
| id | doaj-art-3ee8436a51884ed7b79ffb90119d10db |
| institution | OA Journals |
| issn | 0798-2259 2521-9715 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Universidad del Zulia |
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| series | Revista Científica |
| spelling | doaj-art-3ee8436a51884ed7b79ffb90119d10db2025-08-20T02:05:40ZengUniversidad del ZuliaRevista Científica0798-22592521-97152025-06-01352Liquid chromatography–mass spectrometry phytochemical analysis, antioxidant, anti–inflammatory and analgesic efficacy of Origanum majorana L. aerial part extracts collected from North–Eastern of AlgeriaAhlem Karbab0Noureddine Charef1Salima Amari2Areej Jaber3Ayat Siedat4Alaa Sanabrah5Solomon Derese6Setif-1 University Ferhat Abbas, Faculty of Natural and Life Sciences, Laboratory of Applied Biochemistry. Setif-1, Algeria. Setif-1 University Ferhat Abbas, Faculty of Natural and Life Sciences, Laboratory of Applied Biochemistry. Setif-1, Algeria. Setif-1 University Ferhat Abbas, Faculty of Natural and Life Sciences, Laboratory of Applied Biochemistry. Setif-1, Algeria. Al–Ahliyya Amman University, Faculty of Pharmacy, Pharmacological and Diagnostic Research Center. Amman, Jordan. University of Jordan, Department of Chemistry. Amman, Jordan.Al–Ahliyya Amman University, Faculty of Pharmacy, Pharmacological and Diagnostic Research Center. Amman, Jordan. University of Nairobi, Department of Chemistry. Nairobi, Kenya. Origanum majorana L. (Lamiaceae) has been prescribed in folk medicine for therapeutic purposes such as cancer, asthma, dizziness, rheumatism and inflammatory diseases. To evaluate for the first time the decoction (DecE) and hydro–ethanolic (HEE) extracts of the aerial part of O. majorana. The phytochemical analysis (LC–MS/MS), antioxidant, anti–inflammatory and analgesic potentials were analysed. LC–MS/MS analysis identified 17 components in the extracts. The main compounds detected in the aerial parts of O. majorana include rosmarinic acid (≥ 50%), elagic acid (≥ 20%), indomethacin along with flavonoids, phenolics, benzopyrone, hydroquinones, indolyl acetic acid. Both extracts showed potent antioxidant effects in various antioxidant assay models. Through the eggs protein denaturation assay, both plant extracts showed high in vitro anti–inflammatory effect of the order of 86.91±0.00% for DecE and 85.04±0.00 % for HEE (1.4 mg·mL-1). Acute toxicity measurement revealed no mortality or behavioral changes during the testing period, confirming the two extracts not toxic with an LD50 > 2 g·kg-1. The in vivo anti–inflammatory effects were found to be statistically significant (P<0.05), reducing the edematous response by 78.40 ± 2.52 % and 77.36 ± 3.88 % in DecE and HEE, respectively. Additionally, at a dose of 400.0 mg·kg-1, both extracts exhibited significant analgesics activity (P<0.05) against acetic acid induced abdominal constriction in mice, with inhibition rates of 78.54 ± 3.30% for DecE and 66.99 ± 1.34% for HEE. The results indicate aerial part extracts of O. majorana have potent anti–inflammatory effects and may prove to have potential health benefits. https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43929Origanum majorana Lphytochemical analysistoxicityanti–inflammatory activityanalgesic activity |
| spellingShingle | Ahlem Karbab Noureddine Charef Salima Amari Areej Jaber Ayat Siedat Alaa Sanabrah Solomon Derese Liquid chromatography–mass spectrometry phytochemical analysis, antioxidant, anti–inflammatory and analgesic efficacy of Origanum majorana L. aerial part extracts collected from North–Eastern of Algeria Revista Científica Origanum majorana L phytochemical analysis toxicity anti–inflammatory activity analgesic activity |
| title | Liquid chromatography–mass spectrometry phytochemical analysis, antioxidant, anti–inflammatory and analgesic efficacy of Origanum majorana L. aerial part extracts collected from North–Eastern of Algeria |
| title_full | Liquid chromatography–mass spectrometry phytochemical analysis, antioxidant, anti–inflammatory and analgesic efficacy of Origanum majorana L. aerial part extracts collected from North–Eastern of Algeria |
| title_fullStr | Liquid chromatography–mass spectrometry phytochemical analysis, antioxidant, anti–inflammatory and analgesic efficacy of Origanum majorana L. aerial part extracts collected from North–Eastern of Algeria |
| title_full_unstemmed | Liquid chromatography–mass spectrometry phytochemical analysis, antioxidant, anti–inflammatory and analgesic efficacy of Origanum majorana L. aerial part extracts collected from North–Eastern of Algeria |
| title_short | Liquid chromatography–mass spectrometry phytochemical analysis, antioxidant, anti–inflammatory and analgesic efficacy of Origanum majorana L. aerial part extracts collected from North–Eastern of Algeria |
| title_sort | liquid chromatography mass spectrometry phytochemical analysis antioxidant anti inflammatory and analgesic efficacy of origanum majorana l aerial part extracts collected from north eastern of algeria |
| topic | Origanum majorana L phytochemical analysis toxicity anti–inflammatory activity analgesic activity |
| url | https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43929 |
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