Glycosylation Gene Signatures as Prognostic Biomarkers in Glioblastoma

ABSTRACT Objective Glioblastoma (GBM) is an aggressive brain tumor characterized by significant heterogeneity. This study investigates the role of glycosylation‐related genes in GBM subtyping, prognosis, and response to therapy. Methods We analyzed mRNA expression data and clinical information from...

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Bibliographic Details
Main Authors: Tong Zhao, Hongliang Ge, Chenchao Lin, Xiyue Wu, Jianwu Chen
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Annals of Clinical and Translational Neurology
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Online Access:https://doi.org/10.1002/acn3.70068
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Summary:ABSTRACT Objective Glioblastoma (GBM) is an aggressive brain tumor characterized by significant heterogeneity. This study investigates the role of glycosylation‐related genes in GBM subtyping, prognosis, and response to therapy. Methods We analyzed mRNA expression data and clinical information from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Glycosylation‐related genes were selected for differential expression analysis, sample clustering, and survival analysis. Immune cell infiltration and drug sensitivity were evaluated using CIBERSORT and oncoPredict, respectively. A prognostic model was constructed with Lasso regression. Results GBM samples were stratified into two glycosylation‐related subtypes, showing distinct survival outcomes, with higher glycosylation expression correlating with poorer prognosis. Immune microenvironment analysis revealed differences in T‐cell infiltration and immune checkpoint expression between subtypes, indicating variable immunotherapy responses. The prognostic model based on glycosylation genes demonstrated significant predictive value for patient survival. Conclusion Glycosylation‐related gene expression contributes to GBM heterogeneity and is a valuable biomarker for prognosis and treatment stratification. This study provides insights into personalized treatment approaches for GBM based on glycosylation‐related molecular subtypes.
ISSN:2328-9503