Screening of Antifungal Lactic Acid Bacteria and Their Impact on the Quality and Shelf Life of Rye Bran Sourdough Bread

In the present study, <i>Lactiplantibacillus plantarum</i> G8, exhibiting higher antifungal activity, and G12, displaying weaker antifungal activity, were isolated from naturally fermented wheat sourdough. Their impacts on bread quality and shelf life were subsequently investigated. The...

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Main Authors: Tianyu Mou, Ruixia Xu, Qin Li, Jianlong Li, Shuliang Liu, Xiaolin Ao, Shujuan Chen, Aiping Liu
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/14/7/1253
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author Tianyu Mou
Ruixia Xu
Qin Li
Jianlong Li
Shuliang Liu
Xiaolin Ao
Shujuan Chen
Aiping Liu
author_facet Tianyu Mou
Ruixia Xu
Qin Li
Jianlong Li
Shuliang Liu
Xiaolin Ao
Shujuan Chen
Aiping Liu
author_sort Tianyu Mou
collection DOAJ
description In the present study, <i>Lactiplantibacillus plantarum</i> G8, exhibiting higher antifungal activity, and G12, displaying weaker antifungal activity, were isolated from naturally fermented wheat sourdough. Their impacts on bread quality and shelf life were subsequently investigated. The results demonstrated that both strains exhibited robust growth in rye bran sourdough. Compared to the blank control rye bran–wheat flour dough (RB dough), sourdough incorporation enhanced percentages of β-sheet and α-helix secondary structures, facilitating the formation of a more ordered gluten network structure. This contributed to reduced bread baking loss and decreased bread hardness, gumminess, and chewiness, with <i>Lpb. plantarum</i> G8 exhibiting the most pronounced effects. Notably, G8 bread displayed superior antifungal efficacy, extending shelf life by 8 d (mold appearance at room temperature: 12 d for G8 vs. 4 d for RB). Furthermore, G8 bread exhibited significantly increased diversity and content of volatile compounds, and received higher preference scores from the sensory panel. This study further advances the development of mold-resistant bakery products.
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issn 2304-8158
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publishDate 2025-04-01
publisher MDPI AG
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series Foods
spelling doaj-art-3ee66a38b09a4e42802e27afc1288bd82025-08-20T03:08:53ZengMDPI AGFoods2304-81582025-04-01147125310.3390/foods14071253Screening of Antifungal Lactic Acid Bacteria and Their Impact on the Quality and Shelf Life of Rye Bran Sourdough BreadTianyu Mou0Ruixia Xu1Qin Li2Jianlong Li3Shuliang Liu4Xiaolin Ao5Shujuan Chen6Aiping Liu7College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaIn the present study, <i>Lactiplantibacillus plantarum</i> G8, exhibiting higher antifungal activity, and G12, displaying weaker antifungal activity, were isolated from naturally fermented wheat sourdough. Their impacts on bread quality and shelf life were subsequently investigated. The results demonstrated that both strains exhibited robust growth in rye bran sourdough. Compared to the blank control rye bran–wheat flour dough (RB dough), sourdough incorporation enhanced percentages of β-sheet and α-helix secondary structures, facilitating the formation of a more ordered gluten network structure. This contributed to reduced bread baking loss and decreased bread hardness, gumminess, and chewiness, with <i>Lpb. plantarum</i> G8 exhibiting the most pronounced effects. Notably, G8 bread displayed superior antifungal efficacy, extending shelf life by 8 d (mold appearance at room temperature: 12 d for G8 vs. 4 d for RB). Furthermore, G8 bread exhibited significantly increased diversity and content of volatile compounds, and received higher preference scores from the sensory panel. This study further advances the development of mold-resistant bakery products.https://www.mdpi.com/2304-8158/14/7/1253antifungal LABbreaddough structurerye bran sourdough
spellingShingle Tianyu Mou
Ruixia Xu
Qin Li
Jianlong Li
Shuliang Liu
Xiaolin Ao
Shujuan Chen
Aiping Liu
Screening of Antifungal Lactic Acid Bacteria and Their Impact on the Quality and Shelf Life of Rye Bran Sourdough Bread
Foods
antifungal LAB
bread
dough structure
rye bran sourdough
title Screening of Antifungal Lactic Acid Bacteria and Their Impact on the Quality and Shelf Life of Rye Bran Sourdough Bread
title_full Screening of Antifungal Lactic Acid Bacteria and Their Impact on the Quality and Shelf Life of Rye Bran Sourdough Bread
title_fullStr Screening of Antifungal Lactic Acid Bacteria and Their Impact on the Quality and Shelf Life of Rye Bran Sourdough Bread
title_full_unstemmed Screening of Antifungal Lactic Acid Bacteria and Their Impact on the Quality and Shelf Life of Rye Bran Sourdough Bread
title_short Screening of Antifungal Lactic Acid Bacteria and Their Impact on the Quality and Shelf Life of Rye Bran Sourdough Bread
title_sort screening of antifungal lactic acid bacteria and their impact on the quality and shelf life of rye bran sourdough bread
topic antifungal LAB
bread
dough structure
rye bran sourdough
url https://www.mdpi.com/2304-8158/14/7/1253
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