Interaction of Mycotoxins with α<sub>1</sub>-Acid Glycoprotein (AGP) and Bovine Milk Proteins: Zearalenone, Zearalenols, and Sterigmatocystin Form Highly Stable Complexes with AGP
Mycotoxins are frequent food contaminants posing health risk to humans and animals. Since these interactions have been barely studied yet, we examined the potential complex formation of mycotoxins with human α<sub>1</sub>-acid glycoprotein (AGP) and with bovine milk proteins (including c...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6651/17/4/151 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850180456360706048 |
|---|---|
| author | Miklós Poór Patrik Gömbös András Szabó Balázs Zoltán Zsidó Csaba Hetényi Tamás Huber András Lukács Sándor Kunsági-Máté |
| author_facet | Miklós Poór Patrik Gömbös András Szabó Balázs Zoltán Zsidó Csaba Hetényi Tamás Huber András Lukács Sándor Kunsági-Máté |
| author_sort | Miklós Poór |
| collection | DOAJ |
| description | Mycotoxins are frequent food contaminants posing health risk to humans and animals. Since these interactions have been barely studied yet, we examined the potential complex formation of mycotoxins with human α<sub>1</sub>-acid glycoprotein (AGP) and with bovine milk proteins (including casein (CSN), β-lactoglobulin (LG), and α-lactalbumin (LA)) based on fluorescence spectroscopic and ultracentrifugation techniques. Only weak interactions (log<i>K</i> = 2.7 to 3.5) of certain mycotoxins were observed with CSN, LG, and/or LA. Ultracentrifugation experiments demonstrated that aflatoxin M1, zearalenone, and α-zearalenol form more stable complexes with CSN than with LG or LA. These mycotoxins bound to bovine serum albumin with more than a tenfold higher affinity compared to CSN; nevertheless, it has likely limited importance due to the relatively low levels of BSA in bovine milk. Zearalenone, zearalenols, and sterigmatocystin showed strong interactions with AGP (log<i>K</i> = 5.5 to 6.4), suggesting that AGP may play an important role in the plasma protein binding of these mycotoxins. |
| format | Article |
| id | doaj-art-3ee25a138d594a34b1f4e3c577c8a16b |
| institution | OA Journals |
| issn | 2072-6651 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj-art-3ee25a138d594a34b1f4e3c577c8a16b2025-08-20T02:18:10ZengMDPI AGToxins2072-66512025-03-0117415110.3390/toxins17040151Interaction of Mycotoxins with α<sub>1</sub>-Acid Glycoprotein (AGP) and Bovine Milk Proteins: Zearalenone, Zearalenols, and Sterigmatocystin Form Highly Stable Complexes with AGPMiklós Poór0Patrik Gömbös1András Szabó2Balázs Zoltán Zsidó3Csaba Hetényi4Tamás Huber5András Lukács6Sándor Kunsági-Máté7Department of Laboratory Medicine, Medical School, University of Pécs, Ifjúság útja 13, H-7624 Pécs, HungaryInstitute of Physiology and Nutrition, Department of Physiology and Animal Health, Agribiotechnology and Precision Breeding for Food Security National Laboratory, Hungarian University of Agriculture and Life Sciences, H-2103 Gödöllő, HungaryInstitute of Physiology and Nutrition, Department of Physiology and Animal Health, Agribiotechnology and Precision Breeding for Food Security National Laboratory, Hungarian University of Agriculture and Life Sciences, H-2103 Gödöllő, HungaryPharmacoinformatics Unit, Department of Pharmacology and Pharmacotherapy, Medical School, University of Pécs, Szigeti út 12, H-7624 Pécs, HungaryPharmacoinformatics Unit, Department of Pharmacology and Pharmacotherapy, Medical School, University of Pécs, Szigeti út 12, H-7624 Pécs, HungaryDepartment of Biophysics, Medical School, University of Pécs, Szigeti út 12, H-7624 Pécs, HungaryDepartment of Biophysics, Medical School, University of Pécs, Szigeti út 12, H-7624 Pécs, HungaryDepartment of Organic and Medicinal Chemistry, Faculty of Pharmacy, University of Pécs, Honvéd u. 1, H-7624 Pécs, HungaryMycotoxins are frequent food contaminants posing health risk to humans and animals. Since these interactions have been barely studied yet, we examined the potential complex formation of mycotoxins with human α<sub>1</sub>-acid glycoprotein (AGP) and with bovine milk proteins (including casein (CSN), β-lactoglobulin (LG), and α-lactalbumin (LA)) based on fluorescence spectroscopic and ultracentrifugation techniques. Only weak interactions (log<i>K</i> = 2.7 to 3.5) of certain mycotoxins were observed with CSN, LG, and/or LA. Ultracentrifugation experiments demonstrated that aflatoxin M1, zearalenone, and α-zearalenol form more stable complexes with CSN than with LG or LA. These mycotoxins bound to bovine serum albumin with more than a tenfold higher affinity compared to CSN; nevertheless, it has likely limited importance due to the relatively low levels of BSA in bovine milk. Zearalenone, zearalenols, and sterigmatocystin showed strong interactions with AGP (log<i>K</i> = 5.5 to 6.4), suggesting that AGP may play an important role in the plasma protein binding of these mycotoxins.https://www.mdpi.com/2072-6651/17/4/151mycotoxinszearalenonezearalenolssterigmatocystinaflatoxinsα<sub>1</sub>-acid glycoprotein |
| spellingShingle | Miklós Poór Patrik Gömbös András Szabó Balázs Zoltán Zsidó Csaba Hetényi Tamás Huber András Lukács Sándor Kunsági-Máté Interaction of Mycotoxins with α<sub>1</sub>-Acid Glycoprotein (AGP) and Bovine Milk Proteins: Zearalenone, Zearalenols, and Sterigmatocystin Form Highly Stable Complexes with AGP Toxins mycotoxins zearalenone zearalenols sterigmatocystin aflatoxins α<sub>1</sub>-acid glycoprotein |
| title | Interaction of Mycotoxins with α<sub>1</sub>-Acid Glycoprotein (AGP) and Bovine Milk Proteins: Zearalenone, Zearalenols, and Sterigmatocystin Form Highly Stable Complexes with AGP |
| title_full | Interaction of Mycotoxins with α<sub>1</sub>-Acid Glycoprotein (AGP) and Bovine Milk Proteins: Zearalenone, Zearalenols, and Sterigmatocystin Form Highly Stable Complexes with AGP |
| title_fullStr | Interaction of Mycotoxins with α<sub>1</sub>-Acid Glycoprotein (AGP) and Bovine Milk Proteins: Zearalenone, Zearalenols, and Sterigmatocystin Form Highly Stable Complexes with AGP |
| title_full_unstemmed | Interaction of Mycotoxins with α<sub>1</sub>-Acid Glycoprotein (AGP) and Bovine Milk Proteins: Zearalenone, Zearalenols, and Sterigmatocystin Form Highly Stable Complexes with AGP |
| title_short | Interaction of Mycotoxins with α<sub>1</sub>-Acid Glycoprotein (AGP) and Bovine Milk Proteins: Zearalenone, Zearalenols, and Sterigmatocystin Form Highly Stable Complexes with AGP |
| title_sort | interaction of mycotoxins with α sub 1 sub acid glycoprotein agp and bovine milk proteins zearalenone zearalenols and sterigmatocystin form highly stable complexes with agp |
| topic | mycotoxins zearalenone zearalenols sterigmatocystin aflatoxins α<sub>1</sub>-acid glycoprotein |
| url | https://www.mdpi.com/2072-6651/17/4/151 |
| work_keys_str_mv | AT miklospoor interactionofmycotoxinswithasub1subacidglycoproteinagpandbovinemilkproteinszearalenonezearalenolsandsterigmatocystinformhighlystablecomplexeswithagp AT patrikgombos interactionofmycotoxinswithasub1subacidglycoproteinagpandbovinemilkproteinszearalenonezearalenolsandsterigmatocystinformhighlystablecomplexeswithagp AT andrasszabo interactionofmycotoxinswithasub1subacidglycoproteinagpandbovinemilkproteinszearalenonezearalenolsandsterigmatocystinformhighlystablecomplexeswithagp AT balazszoltanzsido interactionofmycotoxinswithasub1subacidglycoproteinagpandbovinemilkproteinszearalenonezearalenolsandsterigmatocystinformhighlystablecomplexeswithagp AT csabahetenyi interactionofmycotoxinswithasub1subacidglycoproteinagpandbovinemilkproteinszearalenonezearalenolsandsterigmatocystinformhighlystablecomplexeswithagp AT tamashuber interactionofmycotoxinswithasub1subacidglycoproteinagpandbovinemilkproteinszearalenonezearalenolsandsterigmatocystinformhighlystablecomplexeswithagp AT andraslukacs interactionofmycotoxinswithasub1subacidglycoproteinagpandbovinemilkproteinszearalenonezearalenolsandsterigmatocystinformhighlystablecomplexeswithagp AT sandorkunsagimate interactionofmycotoxinswithasub1subacidglycoproteinagpandbovinemilkproteinszearalenonezearalenolsandsterigmatocystinformhighlystablecomplexeswithagp |