Safety evaluation of the food enzyme acetolactate decarboxylase from the genetically modified Bacillus subtilis strain DP‐Ezz65
Abstract The food enzyme acetolactate decarboxylase (α‐acetolactate decarboxylase; EC 4.1.1.5) is produced with the genetically modified Bacillus subtilis strain DP‐Ezz65 by Genencor International B.V. The genetic modifications do not give rise to safety concerns. The production strain meets the req...
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| Language: | English |
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Wiley
2025-05-01
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| Series: | EFSA Journal |
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| Online Access: | https://doi.org/10.2903/j.efsa.2025.9419 |
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| author | EFSA Panel on Food Enzymes (FEZ) Holger Zorn José Manuel Barat Baviera Claudia Bolognesi Francesco Catania Gabriele Gadermaier Ralf Greiner Baltasar Mayo Alicja Mortensen Yrjö Henrik Roos Marize L. M. Solano Monika Sramkova Henk Van Loveren Laurence Vernis Magdalena Andryszkiewicz Daniele Cavanna Cristina Fernàndez‐Fraguas Silvia Peluso Yi Liu |
| author_facet | EFSA Panel on Food Enzymes (FEZ) Holger Zorn José Manuel Barat Baviera Claudia Bolognesi Francesco Catania Gabriele Gadermaier Ralf Greiner Baltasar Mayo Alicja Mortensen Yrjö Henrik Roos Marize L. M. Solano Monika Sramkova Henk Van Loveren Laurence Vernis Magdalena Andryszkiewicz Daniele Cavanna Cristina Fernàndez‐Fraguas Silvia Peluso Yi Liu |
| author_sort | EFSA Panel on Food Enzymes (FEZ) |
| collection | DOAJ |
| description | Abstract The food enzyme acetolactate decarboxylase (α‐acetolactate decarboxylase; EC 4.1.1.5) is produced with the genetically modified Bacillus subtilis strain DP‐Ezz65 by Genencor International B.V. The genetic modifications do not give rise to safety concerns. The production strain meets the requirements for the qualified presumption of safety (QPS) approach. The food enzyme was considered free from viable cells of the production organism and its DNA. It is intended to be used in the processing of cereals and other grains for the production of brewed products. Dietary exposure was estimated to be up to 0.001 mg total organic solids/kg body weight per day in European populations. Given the QPS status of the production strain and the absence of concerns resulting from the food enzyme manufacturing process, toxicity tests were considered unnecessary by the Panel. A search for the homology of the amino acid sequence of the acetolactate decarboxylase to known allergens was made and no match was found. The Panel considered that a risk of allergic reactions upon dietary exposure to the food enzyme cannot be excluded, but that the likelihood is low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns, under the intended conditions of use. |
| format | Article |
| id | doaj-art-3ee104591af347f8b46df43bb2b61357 |
| institution | OA Journals |
| issn | 1831-4732 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | EFSA Journal |
| spelling | doaj-art-3ee104591af347f8b46df43bb2b613572025-08-20T02:00:59ZengWileyEFSA Journal1831-47322025-05-01235n/an/a10.2903/j.efsa.2025.9419Safety evaluation of the food enzyme acetolactate decarboxylase from the genetically modified Bacillus subtilis strain DP‐Ezz65EFSA Panel on Food Enzymes (FEZ)Holger ZornJosé Manuel Barat BavieraClaudia BolognesiFrancesco CataniaGabriele GadermaierRalf GreinerBaltasar MayoAlicja MortensenYrjö Henrik RoosMarize L. M. SolanoMonika SramkovaHenk Van LoverenLaurence VernisMagdalena AndryszkiewiczDaniele CavannaCristina Fernàndez‐FraguasSilvia PelusoYi LiuAbstract The food enzyme acetolactate decarboxylase (α‐acetolactate decarboxylase; EC 4.1.1.5) is produced with the genetically modified Bacillus subtilis strain DP‐Ezz65 by Genencor International B.V. The genetic modifications do not give rise to safety concerns. The production strain meets the requirements for the qualified presumption of safety (QPS) approach. The food enzyme was considered free from viable cells of the production organism and its DNA. It is intended to be used in the processing of cereals and other grains for the production of brewed products. Dietary exposure was estimated to be up to 0.001 mg total organic solids/kg body weight per day in European populations. Given the QPS status of the production strain and the absence of concerns resulting from the food enzyme manufacturing process, toxicity tests were considered unnecessary by the Panel. A search for the homology of the amino acid sequence of the acetolactate decarboxylase to known allergens was made and no match was found. The Panel considered that a risk of allergic reactions upon dietary exposure to the food enzyme cannot be excluded, but that the likelihood is low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns, under the intended conditions of use.https://doi.org/10.2903/j.efsa.2025.9419acetolactate decarboxylaseBacillus subtilisEC 4.1.1.5EFSA‐Q‐2024‐00069food enzymegenetically modified microorganism |
| spellingShingle | EFSA Panel on Food Enzymes (FEZ) Holger Zorn José Manuel Barat Baviera Claudia Bolognesi Francesco Catania Gabriele Gadermaier Ralf Greiner Baltasar Mayo Alicja Mortensen Yrjö Henrik Roos Marize L. M. Solano Monika Sramkova Henk Van Loveren Laurence Vernis Magdalena Andryszkiewicz Daniele Cavanna Cristina Fernàndez‐Fraguas Silvia Peluso Yi Liu Safety evaluation of the food enzyme acetolactate decarboxylase from the genetically modified Bacillus subtilis strain DP‐Ezz65 EFSA Journal acetolactate decarboxylase Bacillus subtilis EC 4.1.1.5 EFSA‐Q‐2024‐00069 food enzyme genetically modified microorganism |
| title | Safety evaluation of the food enzyme acetolactate decarboxylase from the genetically modified Bacillus subtilis strain DP‐Ezz65 |
| title_full | Safety evaluation of the food enzyme acetolactate decarboxylase from the genetically modified Bacillus subtilis strain DP‐Ezz65 |
| title_fullStr | Safety evaluation of the food enzyme acetolactate decarboxylase from the genetically modified Bacillus subtilis strain DP‐Ezz65 |
| title_full_unstemmed | Safety evaluation of the food enzyme acetolactate decarboxylase from the genetically modified Bacillus subtilis strain DP‐Ezz65 |
| title_short | Safety evaluation of the food enzyme acetolactate decarboxylase from the genetically modified Bacillus subtilis strain DP‐Ezz65 |
| title_sort | safety evaluation of the food enzyme acetolactate decarboxylase from the genetically modified bacillus subtilis strain dp ezz65 |
| topic | acetolactate decarboxylase Bacillus subtilis EC 4.1.1.5 EFSA‐Q‐2024‐00069 food enzyme genetically modified microorganism |
| url | https://doi.org/10.2903/j.efsa.2025.9419 |
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