Emergence of multidrug-resistant Mycobacterium tuberculosis of the Beijing lineage in Portugal and Guinea-Bissau: a snapshot of moving clones by whole-genome sequencing
The Beijing genotype comprises a highly disseminated strain type that is frequently associated with multidrug resistant (MDR) tuberculosis (TB) and increased transmissibility but, countries such as Portugal and Guinea-Bissau fall outside the regions phylogeographically associated with this specific...
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| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1774425 |
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| author | João Perdigão Carla Silva Fernando Maltez Diana Machado Anabela Miranda Isabel Couto Paulo Rabna Paola Florez de Sessions Jody Phelan Arnab Pain Ruth McNerney Martin L. Hibberd Igor Mokrousov Taane G. Clark Miguel Viveiros Isabel Portugal |
| author_facet | João Perdigão Carla Silva Fernando Maltez Diana Machado Anabela Miranda Isabel Couto Paulo Rabna Paola Florez de Sessions Jody Phelan Arnab Pain Ruth McNerney Martin L. Hibberd Igor Mokrousov Taane G. Clark Miguel Viveiros Isabel Portugal |
| author_sort | João Perdigão |
| collection | DOAJ |
| description | The Beijing genotype comprises a highly disseminated strain type that is frequently associated with multidrug resistant (MDR) tuberculosis (TB) and increased transmissibility but, countries such as Portugal and Guinea-Bissau fall outside the regions phylogeographically associated with this specific genotype. Nevertheless, recent data shows that this genotype might be gradually emerging in these two countries as an underlying cause of primary MDR-TB. Here, we describe the emergence of Mycobacterium tuberculosis Beijing strains associated with MDR-TB in Portugal and Guinea-Bissau demonstrating the presence of the well described superclusters 100-32 and 94-32 in Portugal and Guinea-Bissau, respectively. Genome-wide analysis and comparison with a global genomic dataset of M. tuberculosis Beijing strains, revealed the presence of two genomic clusters encompassing isolates from Portugal and Guinea-Bissau, GC1 (n = 121) and GC2 (n = 39), both of which bore SNP signatures compatible with the 100-32/B0/W148 and 94-32/Central Asia Outbreak clades, respectively. Moreover, GC2 encompasses a cross-border cluster between Portugal, Guinea-Bissau and Brazil thus supporting migration-associated introduction of MDR-TB and subsequent clonal expansion at the community-level. The comparison with global Beijing datasets demonstrates the global reach of the disease and its complex dissemination across multiple countries while in parallel there are clear microevolutionary trajectories towards extensively drug resistant TB. |
| format | Article |
| id | doaj-art-3edea9799d0240ff88001ab87c3c22d1 |
| institution | DOAJ |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-3edea9799d0240ff88001ab87c3c22d12025-08-20T03:08:32ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01911342135310.1080/22221751.2020.1774425Emergence of multidrug-resistant Mycobacterium tuberculosis of the Beijing lineage in Portugal and Guinea-Bissau: a snapshot of moving clones by whole-genome sequencingJoão Perdigão0Carla Silva1Fernando Maltez2Diana Machado3Anabela Miranda4Isabel Couto5Paulo Rabna6Paola Florez de Sessions7Jody Phelan8Arnab Pain9Ruth McNerney10Martin L. Hibberd11Igor Mokrousov12Taane G. Clark13Miguel Viveiros14Isabel Portugal15iMed.ULisboa – Instituto de Investigação do Medicamento, Faculdade de Farmácia, Universidade de Lisboa, Lisboa, PortugaliMed.ULisboa – Instituto de Investigação do Medicamento, Faculdade de Farmácia, Universidade de Lisboa, Lisboa, PortugalServiço de Doenças Infecciosas, Hospital de Curry Cabral-CHLC, Lisboa, PortugalGlobal Health and Tropical Medicine, GHTM, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, UNL, Lisboa, PortugalNational Mycobacterium Reference Laboratory, Instituto Nacional de Saúde Dr. Ricardo Jorge, Porto, PortugalGlobal Health and Tropical Medicine, GHTM, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, UNL, Lisboa, PortugalInstituto Nacional de Saúde Pública/Projecto de Saúde de Bandim (INASA/PSB), Bissau, Guiné-BissauGenomics Institute Singapore, SingaporeLondon School of Hygiene & Tropical Medicine, London, UKPathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi ArabiaDepartment of Medicine, University of Cape Town, Cape Town, South AfricaLondon School of Hygiene & Tropical Medicine, London, UKLaboratory of Molecular Epidemiology and Evolutionary Genetics, St Petersburg Pasteur Institute, St Petersburg, RussiaLondon School of Hygiene & Tropical Medicine, London, UKGlobal Health and Tropical Medicine, GHTM, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, UNL, Lisboa, PortugaliMed.ULisboa – Instituto de Investigação do Medicamento, Faculdade de Farmácia, Universidade de Lisboa, Lisboa, PortugalThe Beijing genotype comprises a highly disseminated strain type that is frequently associated with multidrug resistant (MDR) tuberculosis (TB) and increased transmissibility but, countries such as Portugal and Guinea-Bissau fall outside the regions phylogeographically associated with this specific genotype. Nevertheless, recent data shows that this genotype might be gradually emerging in these two countries as an underlying cause of primary MDR-TB. Here, we describe the emergence of Mycobacterium tuberculosis Beijing strains associated with MDR-TB in Portugal and Guinea-Bissau demonstrating the presence of the well described superclusters 100-32 and 94-32 in Portugal and Guinea-Bissau, respectively. Genome-wide analysis and comparison with a global genomic dataset of M. tuberculosis Beijing strains, revealed the presence of two genomic clusters encompassing isolates from Portugal and Guinea-Bissau, GC1 (n = 121) and GC2 (n = 39), both of which bore SNP signatures compatible with the 100-32/B0/W148 and 94-32/Central Asia Outbreak clades, respectively. Moreover, GC2 encompasses a cross-border cluster between Portugal, Guinea-Bissau and Brazil thus supporting migration-associated introduction of MDR-TB and subsequent clonal expansion at the community-level. The comparison with global Beijing datasets demonstrates the global reach of the disease and its complex dissemination across multiple countries while in parallel there are clear microevolutionary trajectories towards extensively drug resistant TB.https://www.tandfonline.com/doi/10.1080/22221751.2020.1774425MIRU-VNTRtuberculosismigrationMycobacteriaBeijingMtbC15-9 94-32 |
| spellingShingle | João Perdigão Carla Silva Fernando Maltez Diana Machado Anabela Miranda Isabel Couto Paulo Rabna Paola Florez de Sessions Jody Phelan Arnab Pain Ruth McNerney Martin L. Hibberd Igor Mokrousov Taane G. Clark Miguel Viveiros Isabel Portugal Emergence of multidrug-resistant Mycobacterium tuberculosis of the Beijing lineage in Portugal and Guinea-Bissau: a snapshot of moving clones by whole-genome sequencing Emerging Microbes and Infections MIRU-VNTR tuberculosis migration Mycobacteria Beijing MtbC15-9 94-32 |
| title | Emergence of multidrug-resistant Mycobacterium tuberculosis of the Beijing lineage in Portugal and Guinea-Bissau: a snapshot of moving clones by whole-genome sequencing |
| title_full | Emergence of multidrug-resistant Mycobacterium tuberculosis of the Beijing lineage in Portugal and Guinea-Bissau: a snapshot of moving clones by whole-genome sequencing |
| title_fullStr | Emergence of multidrug-resistant Mycobacterium tuberculosis of the Beijing lineage in Portugal and Guinea-Bissau: a snapshot of moving clones by whole-genome sequencing |
| title_full_unstemmed | Emergence of multidrug-resistant Mycobacterium tuberculosis of the Beijing lineage in Portugal and Guinea-Bissau: a snapshot of moving clones by whole-genome sequencing |
| title_short | Emergence of multidrug-resistant Mycobacterium tuberculosis of the Beijing lineage in Portugal and Guinea-Bissau: a snapshot of moving clones by whole-genome sequencing |
| title_sort | emergence of multidrug resistant mycobacterium tuberculosis of the beijing lineage in portugal and guinea bissau a snapshot of moving clones by whole genome sequencing |
| topic | MIRU-VNTR tuberculosis migration Mycobacteria Beijing MtbC15-9 94-32 |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1774425 |
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