Structural Diversity of Mitochondria in the Neuromuscular System across Development Revealed by 3D Electron Microscopy
Abstract As an animal matures, its neural circuit undergoes alterations, yet the developmental changes in intracellular organelles to facilitate these changes is less understood. Using 3D electron microscopy and deep learning, the study develops semi‐automated methods for reconstructing mitochondria...
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| Format: | Article |
| Language: | English |
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Wiley
2025-05-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202411191 |
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| author | J. Alexander Bae Myung‐kyu Choi Soungyub Ahn Gwanho Ko Daniel T. Choe Hyunsoo Yim Ken C. Nguyen Jinseop S. Kim David H. Hall Junho Lee |
| author_facet | J. Alexander Bae Myung‐kyu Choi Soungyub Ahn Gwanho Ko Daniel T. Choe Hyunsoo Yim Ken C. Nguyen Jinseop S. Kim David H. Hall Junho Lee |
| author_sort | J. Alexander Bae |
| collection | DOAJ |
| description | Abstract As an animal matures, its neural circuit undergoes alterations, yet the developmental changes in intracellular organelles to facilitate these changes is less understood. Using 3D electron microscopy and deep learning, the study develops semi‐automated methods for reconstructing mitochondria in C. elegans and collected mitochondria reconstructions from normal reproductive stages and dauer, enabling comparative study on mitochondria structure within the neuromuscular system. It is found that various mitochondria structural properties in neurons correlate with synaptic connections and these properties are preserved across development in different neural circuits. To test the necessity of these universal mitochondria properties, the study examines the behavior in drp‐1 mutants with impaired mitochondria fission and discovers that it causes behavioral deficits. Moreover, it is observed that dauer neurons display distinctive mitochondrial features, and mitochondria in dauer muscles exhibit unique reticulum‐like structure. It is proposed that these specialized mitochondria structures may serve as an adaptive mechanism to support stage‐specific behavioral and physiological needs. |
| format | Article |
| id | doaj-art-3edcd941041247fca628f0bb8fcc985f |
| institution | DOAJ |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-3edcd941041247fca628f0bb8fcc985f2025-08-20T03:20:10ZengWileyAdvanced Science2198-38442025-05-011220n/an/a10.1002/advs.202411191Structural Diversity of Mitochondria in the Neuromuscular System across Development Revealed by 3D Electron MicroscopyJ. Alexander Bae0Myung‐kyu Choi1Soungyub Ahn2Gwanho Ko3Daniel T. Choe4Hyunsoo Yim5Ken C. Nguyen6Jinseop S. Kim7David H. Hall8Junho Lee9Research Institute of Basic Sciences Seoul National University Seoul 08826 Republic of KoreaDepartment of Biological Sciences Seoul National University Seoul 08826 Republic of KoreaDepartment of Biological Sciences Seoul National University Seoul 08826 Republic of KoreaDepartment of Biological Sciences Seoul National University Seoul 08826 Republic of KoreaDepartment of Biological Sciences Seoul National University Seoul 08826 Republic of KoreaDepartment of Biological Sciences Seoul National University Seoul 08826 Republic of KoreaDominick P. Purpura Department of Neuroscience Albert Einstein College of Medicine Bronx NY 10461 USADepartment of Biological Sciences Sungkyunkwan University Suwon 16419 Republic of KoreaDominick P. Purpura Department of Neuroscience Albert Einstein College of Medicine Bronx NY 10461 USAResearch Institute of Basic Sciences Seoul National University Seoul 08826 Republic of KoreaAbstract As an animal matures, its neural circuit undergoes alterations, yet the developmental changes in intracellular organelles to facilitate these changes is less understood. Using 3D electron microscopy and deep learning, the study develops semi‐automated methods for reconstructing mitochondria in C. elegans and collected mitochondria reconstructions from normal reproductive stages and dauer, enabling comparative study on mitochondria structure within the neuromuscular system. It is found that various mitochondria structural properties in neurons correlate with synaptic connections and these properties are preserved across development in different neural circuits. To test the necessity of these universal mitochondria properties, the study examines the behavior in drp‐1 mutants with impaired mitochondria fission and discovers that it causes behavioral deficits. Moreover, it is observed that dauer neurons display distinctive mitochondrial features, and mitochondria in dauer muscles exhibit unique reticulum‐like structure. It is proposed that these specialized mitochondria structures may serve as an adaptive mechanism to support stage‐specific behavioral and physiological needs.https://doi.org/10.1002/advs.202411191C. elegansdeep learningdevelopmentelectron microscopyfluorescence imagingmitochondria |
| spellingShingle | J. Alexander Bae Myung‐kyu Choi Soungyub Ahn Gwanho Ko Daniel T. Choe Hyunsoo Yim Ken C. Nguyen Jinseop S. Kim David H. Hall Junho Lee Structural Diversity of Mitochondria in the Neuromuscular System across Development Revealed by 3D Electron Microscopy Advanced Science C. elegans deep learning development electron microscopy fluorescence imaging mitochondria |
| title | Structural Diversity of Mitochondria in the Neuromuscular System across Development Revealed by 3D Electron Microscopy |
| title_full | Structural Diversity of Mitochondria in the Neuromuscular System across Development Revealed by 3D Electron Microscopy |
| title_fullStr | Structural Diversity of Mitochondria in the Neuromuscular System across Development Revealed by 3D Electron Microscopy |
| title_full_unstemmed | Structural Diversity of Mitochondria in the Neuromuscular System across Development Revealed by 3D Electron Microscopy |
| title_short | Structural Diversity of Mitochondria in the Neuromuscular System across Development Revealed by 3D Electron Microscopy |
| title_sort | structural diversity of mitochondria in the neuromuscular system across development revealed by 3d electron microscopy |
| topic | C. elegans deep learning development electron microscopy fluorescence imaging mitochondria |
| url | https://doi.org/10.1002/advs.202411191 |
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