Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study
This study aimed at examining the early effects of hyperbaric oxygen therapy (HBOT) on inducible nitric oxide synthase (iNOS) activity/expression in lymphocytes of type 1 diabetes mellitus (T1DM) patients. A group of 19 patients (mean age: 63 ± 2.1) with T1DM and with the peripheral arterial disease...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2019/2328505 |
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| author | Ivana Resanovic Zoran Gluvic Bozidarka Zaric Emina Sudar-Milovanovic Aleksandra Jovanovic Davorka Milacic Radmilo Isakovic Esma R. Isenovic |
| author_facet | Ivana Resanovic Zoran Gluvic Bozidarka Zaric Emina Sudar-Milovanovic Aleksandra Jovanovic Davorka Milacic Radmilo Isakovic Esma R. Isenovic |
| author_sort | Ivana Resanovic |
| collection | DOAJ |
| description | This study aimed at examining the early effects of hyperbaric oxygen therapy (HBOT) on inducible nitric oxide synthase (iNOS) activity/expression in lymphocytes of type 1 diabetes mellitus (T1DM) patients. A group of 19 patients (mean age: 63 ± 2.1) with T1DM and with the peripheral arterial disease were included in this study. Patients were exposed to 10 sessions of HBOT in the duration of 1 h to 100% oxygen inhalation at 2.4 ATA. Blood samples were collected for the plasma C-reactive protein (CRP), plasma free fatty acid (FFA), serum nitrite/nitrate, and serum arginase activity measurements. Expression of iNOS and phosphorylation of p65 subunit of nuclear factor-κB (NFκB-p65), extracellular-regulated kinases 1/2 (ERK1/2), and protein kinase B (Akt) were examined in lymphocyte lysates by Western blot. After exposure to HBOT, plasma CRP and FFA were significantly decreased (p<0.001). Protein expression of iNOS and serum nitrite/nitrate levels were decreased (p<0.01), while serum arginase activity was increased (p<0.05) versus before exposure to HBOT. Increased phosphorylation of NFκB-p65 at Ser536 (p<0.05) and decreased level of NFκB-p65 protein (p<0.001) in lymphocytes of T1DM patients were observed after HBOT. Decreased phosphorylation of ERK1/2 (p<0.05) and Akt (p<0.05) was detected after HBOT. Our results indicate that exposure to HBO decreased iNOS activity/expression via decreasing phosphorylation of ERK1/2 and Akt followed by decreased activity of NFκB. |
| format | Article |
| id | doaj-art-3edc125acaf849349367086008239228 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-3edc125acaf8493493670860082392282025-02-03T05:59:38ZengWileyInternational Journal of Endocrinology1687-83371687-83452019-01-01201910.1155/2019/23285052328505Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot StudyIvana Resanovic0Zoran Gluvic1Bozidarka Zaric2Emina Sudar-Milovanovic3Aleksandra Jovanovic4Davorka Milacic5Radmilo Isakovic6Esma R. Isenovic7Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Belgrade, SerbiaClinic for Internal Medicine, Zemun Clinical Hospital, School of Medicine, University of Belgrade, Belgrade, SerbiaInstitute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Belgrade, SerbiaInstitute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Belgrade, SerbiaInstitute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Belgrade, SerbiaDepartment of Hyperbaric Medicine, Zemun Clinical Hospital, Belgrade, SerbiaDepartment of Hyperbaric Medicine, Zemun Clinical Hospital, Belgrade, SerbiaInstitute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Belgrade, SerbiaThis study aimed at examining the early effects of hyperbaric oxygen therapy (HBOT) on inducible nitric oxide synthase (iNOS) activity/expression in lymphocytes of type 1 diabetes mellitus (T1DM) patients. A group of 19 patients (mean age: 63 ± 2.1) with T1DM and with the peripheral arterial disease were included in this study. Patients were exposed to 10 sessions of HBOT in the duration of 1 h to 100% oxygen inhalation at 2.4 ATA. Blood samples were collected for the plasma C-reactive protein (CRP), plasma free fatty acid (FFA), serum nitrite/nitrate, and serum arginase activity measurements. Expression of iNOS and phosphorylation of p65 subunit of nuclear factor-κB (NFκB-p65), extracellular-regulated kinases 1/2 (ERK1/2), and protein kinase B (Akt) were examined in lymphocyte lysates by Western blot. After exposure to HBOT, plasma CRP and FFA were significantly decreased (p<0.001). Protein expression of iNOS and serum nitrite/nitrate levels were decreased (p<0.01), while serum arginase activity was increased (p<0.05) versus before exposure to HBOT. Increased phosphorylation of NFκB-p65 at Ser536 (p<0.05) and decreased level of NFκB-p65 protein (p<0.001) in lymphocytes of T1DM patients were observed after HBOT. Decreased phosphorylation of ERK1/2 (p<0.05) and Akt (p<0.05) was detected after HBOT. Our results indicate that exposure to HBO decreased iNOS activity/expression via decreasing phosphorylation of ERK1/2 and Akt followed by decreased activity of NFκB.http://dx.doi.org/10.1155/2019/2328505 |
| spellingShingle | Ivana Resanovic Zoran Gluvic Bozidarka Zaric Emina Sudar-Milovanovic Aleksandra Jovanovic Davorka Milacic Radmilo Isakovic Esma R. Isenovic Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study International Journal of Endocrinology |
| title | Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study |
| title_full | Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study |
| title_fullStr | Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study |
| title_full_unstemmed | Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study |
| title_short | Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study |
| title_sort | early effects of hyperbaric oxygen on inducible nitric oxide synthase activity expression in lymphocytes of type 1 diabetes patients a prospective pilot study |
| url | http://dx.doi.org/10.1155/2019/2328505 |
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