Genetic alterations in B-cell non-Hodgkin's lymphoma
Background. Although the patients with diagnosed B-NHL are classified into the same disease stage on the basis of clinical, histopathological, and immunological parameters, they respond significantly different to the applied treatment. This points out the possibility that within the same group of ly...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2005-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2005/0042-84500502087M.pdf |
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| author | Magić Zvonko Novković Tamara Cikota Bojana Tasić-Radić Olga Tarabar Olivera Stamatović Dragana |
| author_facet | Magić Zvonko Novković Tamara Cikota Bojana Tasić-Radić Olga Tarabar Olivera Stamatović Dragana |
| author_sort | Magić Zvonko |
| collection | DOAJ |
| description | Background. Although the patients with diagnosed B-NHL are classified into the same disease stage on the basis of clinical, histopathological, and immunological parameters, they respond significantly different to the applied treatment. This points out the possibility that within the same group of lymphoma there are different diseases at molecular level. For that reason many studies deal with the detection of gene alterations in lymphomas to provide a better framework for diagnosis and treatment of these hematological malignancies. Aim. To define genetic alterations in the B-NHL with highest possibilities for diagnostic purposes and molecular detection of MRD. Methods. Formalin fixed and paraffin embedded lymph node tissues from 45 patients were examined by different PCR techniques for the presence of IgH and TCR γ gene rearrangement; K-ras and H-ras mutations; c-myc amplification and bcl-2 translocation. There were 34 cases of B-cell non-Hodgkin’s lymphoma (B-NHL), 5 cases of T-cell non-Hodgkin’s lymphoma (T-NHL) and 6 cases of chronic lymphadenitis (CL). The mononuclear cell fraction of the peripheral blood of 12 patients with B-NHL was analyzed for the presence of monoclonality at the time of diagnosis and in 3 to 6 months time intervals after an autologous bone marrow transplantation (BMT). Results. The monoclonality of B-lymphocytes, as evidenced by DNA fragment length homogeneity, was detected in 88 % (30/34) of B-NHL, but never in CL, T-NHL, or in normal PBL. Bcl-2 translocation was detected in 7/31 (22.6%) B-NHL specimens, c-myc amplification 9/31 (29%, all were more than doubled), K-ras mutations in 1/31 (3.23%) and H-ras mutations in 2/31 (6.45%) of the examined B-NHL samples. In the case of LC and normal PBL, however, these gene alterations were not detected. All the patients (12) with B-NHL had dominant clone of B-lymphocyte in the peripheral blood at the time of diagnosis while only in 2 of 12 patients MRD was detected 3 or 6 months after BMT. Conclusion. Because it is quick and simple, PCR analysis of clonal IgH rearrangements is very useful when diagnostic assistance is required. This technique is also very efficient for tracking minimal residual disease in lymphomas and leukemia's and for monitoring clonal evolution in acute and chronic lymphoblastic leukemia's and lymphomas. The presence of other genetic alterations, which we detected, should serve as an additional prognostic or predictive factor in the patients with B-NHL. |
| format | Article |
| id | doaj-art-3ebdfddc862c4d9cbec983c355d75b65 |
| institution | OA Journals |
| issn | 0042-8450 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-3ebdfddc862c4d9cbec983c355d75b652025-08-20T01:56:25ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502005-01-01622879610.2298/VSP0502087MGenetic alterations in B-cell non-Hodgkin's lymphomaMagić ZvonkoNovković TamaraCikota BojanaTasić-Radić OlgaTarabar OliveraStamatović DraganaBackground. Although the patients with diagnosed B-NHL are classified into the same disease stage on the basis of clinical, histopathological, and immunological parameters, they respond significantly different to the applied treatment. This points out the possibility that within the same group of lymphoma there are different diseases at molecular level. For that reason many studies deal with the detection of gene alterations in lymphomas to provide a better framework for diagnosis and treatment of these hematological malignancies. Aim. To define genetic alterations in the B-NHL with highest possibilities for diagnostic purposes and molecular detection of MRD. Methods. Formalin fixed and paraffin embedded lymph node tissues from 45 patients were examined by different PCR techniques for the presence of IgH and TCR γ gene rearrangement; K-ras and H-ras mutations; c-myc amplification and bcl-2 translocation. There were 34 cases of B-cell non-Hodgkin’s lymphoma (B-NHL), 5 cases of T-cell non-Hodgkin’s lymphoma (T-NHL) and 6 cases of chronic lymphadenitis (CL). The mononuclear cell fraction of the peripheral blood of 12 patients with B-NHL was analyzed for the presence of monoclonality at the time of diagnosis and in 3 to 6 months time intervals after an autologous bone marrow transplantation (BMT). Results. The monoclonality of B-lymphocytes, as evidenced by DNA fragment length homogeneity, was detected in 88 % (30/34) of B-NHL, but never in CL, T-NHL, or in normal PBL. Bcl-2 translocation was detected in 7/31 (22.6%) B-NHL specimens, c-myc amplification 9/31 (29%, all were more than doubled), K-ras mutations in 1/31 (3.23%) and H-ras mutations in 2/31 (6.45%) of the examined B-NHL samples. In the case of LC and normal PBL, however, these gene alterations were not detected. All the patients (12) with B-NHL had dominant clone of B-lymphocyte in the peripheral blood at the time of diagnosis while only in 2 of 12 patients MRD was detected 3 or 6 months after BMT. Conclusion. Because it is quick and simple, PCR analysis of clonal IgH rearrangements is very useful when diagnostic assistance is required. This technique is also very efficient for tracking minimal residual disease in lymphomas and leukemia's and for monitoring clonal evolution in acute and chronic lymphoblastic leukemia's and lymphomas. The presence of other genetic alterations, which we detected, should serve as an additional prognostic or predictive factor in the patients with B-NHL.http://www.doiserbia.nb.rs/img/doi/0042-8450/2005/0042-84500502087M.pdflymphomanon-Hodgkingenetic phenomenamutationpolymerase chain reaction |
| spellingShingle | Magić Zvonko Novković Tamara Cikota Bojana Tasić-Radić Olga Tarabar Olivera Stamatović Dragana Genetic alterations in B-cell non-Hodgkin's lymphoma Vojnosanitetski Pregled lymphoma non-Hodgkin genetic phenomena mutation polymerase chain reaction |
| title | Genetic alterations in B-cell non-Hodgkin's lymphoma |
| title_full | Genetic alterations in B-cell non-Hodgkin's lymphoma |
| title_fullStr | Genetic alterations in B-cell non-Hodgkin's lymphoma |
| title_full_unstemmed | Genetic alterations in B-cell non-Hodgkin's lymphoma |
| title_short | Genetic alterations in B-cell non-Hodgkin's lymphoma |
| title_sort | genetic alterations in b cell non hodgkin s lymphoma |
| topic | lymphoma non-Hodgkin genetic phenomena mutation polymerase chain reaction |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2005/0042-84500502087M.pdf |
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