Mechanism of Enhancing the Inhibitory Effect of Metschnikowia rubicola on Apple Penicilliosis by Cofactors
The Metschnikowia rubicola obtained from laboratory screening could effectively inhibit the occurrence of postharvest apple penicillium rot. In order to improve the inhibitory effect of M. rubicola on apple penicillium rot, cofactors with M. rubicola were screened. The mechanism of cofactors in enha...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2025-08-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090161 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849340471983210496 |
|---|---|
| author | Jingting DU Nan LIN Longmei CONG Junyue HU Junfeng SHI Yuqing GE Qian ZHANG |
| author_facet | Jingting DU Nan LIN Longmei CONG Junyue HU Junfeng SHI Yuqing GE Qian ZHANG |
| author_sort | Jingting DU |
| collection | DOAJ |
| description | The Metschnikowia rubicola obtained from laboratory screening could effectively inhibit the occurrence of postharvest apple penicillium rot. In order to improve the inhibitory effect of M. rubicola on apple penicillium rot, cofactors with M. rubicola were screened. The mechanism of cofactors in enhancing the resistance of M. rubicola to penicilliosis was explored by compared the activity of M. rubicola, the spore germination and hyphal growth of Penicillium expansum, the activity of resistance related enzymes, and the degradation of PAT before and after adding cofactors. The results showed that the addition of 5 g/L AOS or 4 μmol/L PA could effectively improve the control effect of M. rubicola on apple penicilliosis and reduce the spore germination and mycelial growth rate of P. expansum. The addition of AOS was more significant, resulting in inhibition rate increases of 21.67% and 31.40%, respectively. AOS or PA could also enhance M. rubicola's colonization ability on apple wounds and surfaces. Increased the activity of enzymes related to the phenylpropane metabolic pathway (PPO, POD, PAL, CAD, C4H, 4CL, CHI, GLU) and the content of lignin, total phenols, and flavonoids significantly on apples. M. rubicola induced by AOS could enhance its ability to degrade PAT. Overall, the addition of AOS or PA could effectively enhance the preventive and therapeutic effects of M. rubicola on apple penicillium disease, and AOS could also enhance M Rubicola's ability to degrade PAT. |
| format | Article |
| id | doaj-art-3eb62800d9f04bfd9fdb512247d537aa |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-08-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-3eb62800d9f04bfd9fdb512247d537aa2025-08-20T03:43:55ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-08-01461636537410.13386/j.issn1002-0306.20240901612024090161-16Mechanism of Enhancing the Inhibitory Effect of Metschnikowia rubicola on Apple Penicilliosis by CofactorsJingting DU0Nan LIN1Longmei CONG2Junyue HU3Junfeng SHI4Yuqing GE5Qian ZHANG6School of Food Scince and Engineering, Shanxi Agricultural University, Taiyuan 030031, ChinaSchool of Food Scince and Engineering, Shanxi Agricultural University, Taiyuan 030031, ChinaSchool of Food Scince and Engineering, Shanxi Agricultural University, Taiyuan 030031, ChinaSchool of Food Scince and Engineering, Shanxi Agricultural University, Taiyuan 030031, ChinaSchool of Food Scince and Engineering, Shanxi Agricultural University, Taiyuan 030031, ChinaState Key Laboratory of Sensing Technology, Shanghai Institute of Microsystems and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaSchool of Food Scince and Engineering, Shanxi Agricultural University, Taiyuan 030031, ChinaThe Metschnikowia rubicola obtained from laboratory screening could effectively inhibit the occurrence of postharvest apple penicillium rot. In order to improve the inhibitory effect of M. rubicola on apple penicillium rot, cofactors with M. rubicola were screened. The mechanism of cofactors in enhancing the resistance of M. rubicola to penicilliosis was explored by compared the activity of M. rubicola, the spore germination and hyphal growth of Penicillium expansum, the activity of resistance related enzymes, and the degradation of PAT before and after adding cofactors. The results showed that the addition of 5 g/L AOS or 4 μmol/L PA could effectively improve the control effect of M. rubicola on apple penicilliosis and reduce the spore germination and mycelial growth rate of P. expansum. The addition of AOS was more significant, resulting in inhibition rate increases of 21.67% and 31.40%, respectively. AOS or PA could also enhance M. rubicola's colonization ability on apple wounds and surfaces. Increased the activity of enzymes related to the phenylpropane metabolic pathway (PPO, POD, PAL, CAD, C4H, 4CL, CHI, GLU) and the content of lignin, total phenols, and flavonoids significantly on apples. M. rubicola induced by AOS could enhance its ability to degrade PAT. Overall, the addition of AOS or PA could effectively enhance the preventive and therapeutic effects of M. rubicola on apple penicillium disease, and AOS could also enhance M Rubicola's ability to degrade PAT.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090161applepenicilliosismetschnikowia rubicolacofactors |
| spellingShingle | Jingting DU Nan LIN Longmei CONG Junyue HU Junfeng SHI Yuqing GE Qian ZHANG Mechanism of Enhancing the Inhibitory Effect of Metschnikowia rubicola on Apple Penicilliosis by Cofactors Shipin gongye ke-ji apple penicilliosis metschnikowia rubicola cofactors |
| title | Mechanism of Enhancing the Inhibitory Effect of Metschnikowia rubicola on Apple Penicilliosis by Cofactors |
| title_full | Mechanism of Enhancing the Inhibitory Effect of Metschnikowia rubicola on Apple Penicilliosis by Cofactors |
| title_fullStr | Mechanism of Enhancing the Inhibitory Effect of Metschnikowia rubicola on Apple Penicilliosis by Cofactors |
| title_full_unstemmed | Mechanism of Enhancing the Inhibitory Effect of Metschnikowia rubicola on Apple Penicilliosis by Cofactors |
| title_short | Mechanism of Enhancing the Inhibitory Effect of Metschnikowia rubicola on Apple Penicilliosis by Cofactors |
| title_sort | mechanism of enhancing the inhibitory effect of metschnikowia rubicola on apple penicilliosis by cofactors |
| topic | apple penicilliosis metschnikowia rubicola cofactors |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090161 |
| work_keys_str_mv | AT jingtingdu mechanismofenhancingtheinhibitoryeffectofmetschnikowiarubicolaonapplepenicilliosisbycofactors AT nanlin mechanismofenhancingtheinhibitoryeffectofmetschnikowiarubicolaonapplepenicilliosisbycofactors AT longmeicong mechanismofenhancingtheinhibitoryeffectofmetschnikowiarubicolaonapplepenicilliosisbycofactors AT junyuehu mechanismofenhancingtheinhibitoryeffectofmetschnikowiarubicolaonapplepenicilliosisbycofactors AT junfengshi mechanismofenhancingtheinhibitoryeffectofmetschnikowiarubicolaonapplepenicilliosisbycofactors AT yuqingge mechanismofenhancingtheinhibitoryeffectofmetschnikowiarubicolaonapplepenicilliosisbycofactors AT qianzhang mechanismofenhancingtheinhibitoryeffectofmetschnikowiarubicolaonapplepenicilliosisbycofactors |