Convergence of root ectomycorrhizal fungal communities across Pinaceae developmental stages in temperate forests in northeastern China
Summary: Ectomycorrhizal fungi (EMF) are crucial for the development of temperate forests. We conducted a field study via a “space-for-time” approach to investigate root EMF succession across saplings, juveniles, and adults of four Pinaceae species in northeastern China. By high-throughput sequencin...
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Elsevier
2025-06-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225010156 |
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| author | Dongxue Zhao Yulian Wei Ting Cao Yiwei Yuan Jiarui Yu Zhen Bai Haisheng Yuan |
| author_facet | Dongxue Zhao Yulian Wei Ting Cao Yiwei Yuan Jiarui Yu Zhen Bai Haisheng Yuan |
| author_sort | Dongxue Zhao |
| collection | DOAJ |
| description | Summary: Ectomycorrhizal fungi (EMF) are crucial for the development of temperate forests. We conducted a field study via a “space-for-time” approach to investigate root EMF succession across saplings, juveniles, and adults of four Pinaceae species in northeastern China. By high-throughput sequencing of the fungal internal transcribed spacer 2 region, we cataloged 1,641 EMF amplicon sequence variants. As Pinaceae matured, EMF alpha diversity notably increased, while their Bray-Curtis dissimilarity significantly decreased. A random forest algorithm identified stage-specific taxa, marking the age-related transition from structural divergence to similarity in root EMF communities. EMF genera Amphinema and Suillus dominated in Pinaceae saplings, while Piloderma, Russula, Sebacina, and Tomentella were more common in Pinaceae adults. Co-occurrence network analysis demonstrated greater interconnectivity among EMF taxa in adults than in their younger counterparts. These findings suggest that root EMF community dynamics are synchronized with the recruitment and development of Pinaceae species in temperate forests. |
| format | Article |
| id | doaj-art-3ead46a3f3374f88a47cedb1cf347abf |
| institution | DOAJ |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-3ead46a3f3374f88a47cedb1cf347abf2025-08-20T03:10:20ZengElsevieriScience2589-00422025-06-0128611275410.1016/j.isci.2025.112754Convergence of root ectomycorrhizal fungal communities across Pinaceae developmental stages in temperate forests in northeastern ChinaDongxue Zhao0Yulian Wei1Ting Cao2Yiwei Yuan3Jiarui Yu4Zhen Bai5Haisheng Yuan6CAS Key Laboratory of Forest Ecology and Silviculture, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P.R. China; University of the Chinese Academy of Sciences, Beijing 100049, P.R. ChinaCAS Key Laboratory of Forest Ecology and Silviculture, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P.R. ChinaSchool of Public Health, Shenyang Medical College, Shenyang 110034, P.R. ChinaCollege of Life Sciences, Shanxi Agricultural University, Taigu, Shanxi 030801, P.R. ChinaCAS Key Laboratory of Forest Ecology and Silviculture, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P.R. China; University of the Chinese Academy of Sciences, Beijing 100049, P.R. ChinaCAS Key Laboratory of Forest Ecology and Silviculture, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P.R. China; Corresponding authorCAS Key Laboratory of Forest Ecology and Silviculture, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P.R. China; Corresponding authorSummary: Ectomycorrhizal fungi (EMF) are crucial for the development of temperate forests. We conducted a field study via a “space-for-time” approach to investigate root EMF succession across saplings, juveniles, and adults of four Pinaceae species in northeastern China. By high-throughput sequencing of the fungal internal transcribed spacer 2 region, we cataloged 1,641 EMF amplicon sequence variants. As Pinaceae matured, EMF alpha diversity notably increased, while their Bray-Curtis dissimilarity significantly decreased. A random forest algorithm identified stage-specific taxa, marking the age-related transition from structural divergence to similarity in root EMF communities. EMF genera Amphinema and Suillus dominated in Pinaceae saplings, while Piloderma, Russula, Sebacina, and Tomentella were more common in Pinaceae adults. Co-occurrence network analysis demonstrated greater interconnectivity among EMF taxa in adults than in their younger counterparts. These findings suggest that root EMF community dynamics are synchronized with the recruitment and development of Pinaceae species in temperate forests.http://www.sciencedirect.com/science/article/pii/S2589004225010156GeneticsMycology |
| spellingShingle | Dongxue Zhao Yulian Wei Ting Cao Yiwei Yuan Jiarui Yu Zhen Bai Haisheng Yuan Convergence of root ectomycorrhizal fungal communities across Pinaceae developmental stages in temperate forests in northeastern China iScience Genetics Mycology |
| title | Convergence of root ectomycorrhizal fungal communities across Pinaceae developmental stages in temperate forests in northeastern China |
| title_full | Convergence of root ectomycorrhizal fungal communities across Pinaceae developmental stages in temperate forests in northeastern China |
| title_fullStr | Convergence of root ectomycorrhizal fungal communities across Pinaceae developmental stages in temperate forests in northeastern China |
| title_full_unstemmed | Convergence of root ectomycorrhizal fungal communities across Pinaceae developmental stages in temperate forests in northeastern China |
| title_short | Convergence of root ectomycorrhizal fungal communities across Pinaceae developmental stages in temperate forests in northeastern China |
| title_sort | convergence of root ectomycorrhizal fungal communities across pinaceae developmental stages in temperate forests in northeastern china |
| topic | Genetics Mycology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225010156 |
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