Whole exome sequencing shows novel COL4A3 and COL4A4 variants as causes of Alport syndrome in Rio Grande do Norte, Brazil
Abstract Background Alport syndrome is a progressive and hereditary nephropathy characterized by hematuria and proteinuria as well as extra renal manifestations as hearing loss and eye abnormalities. The disease can be expressed as autosomal recessive or autosomal dominant at COL4A3 and COL4A4 loci,...
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2025-04-01
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| Online Access: | https://doi.org/10.1186/s12864-025-11466-4 |
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| author | Washington Candeia de Araújo Raul Maia Falcão Raquel Araujo Costa Uchoa Carlos Alexandre Garcia Arthur Quintiliano Bezerra da Silva Kesia Larissa Medeiros Quirino Francisco Paulo Freire-Neto Genilson Pereira Gurgel Paulo Ricardo Porfirio Nascimento Leonardo Capistrano Ferreira Priya Duggal Jorge Estefano S. de Souza Selma M. B. Jeronimo |
| author_facet | Washington Candeia de Araújo Raul Maia Falcão Raquel Araujo Costa Uchoa Carlos Alexandre Garcia Arthur Quintiliano Bezerra da Silva Kesia Larissa Medeiros Quirino Francisco Paulo Freire-Neto Genilson Pereira Gurgel Paulo Ricardo Porfirio Nascimento Leonardo Capistrano Ferreira Priya Duggal Jorge Estefano S. de Souza Selma M. B. Jeronimo |
| author_sort | Washington Candeia de Araújo |
| collection | DOAJ |
| description | Abstract Background Alport syndrome is a progressive and hereditary nephropathy characterized by hematuria and proteinuria as well as extra renal manifestations as hearing loss and eye abnormalities. The disease can be expressed as autosomal recessive or autosomal dominant at COL4A3 and COL4A4 loci, respectively, or X-linked at the COL4A5 locus. This study investigated two unrelated families with nephropathy from Brazil with the aim to identify the mutations involved with the disease. Methods Whole Exome Sequencing was performed for 4 people from each pedigree (case, parents and a sibling). DNA sequences were mapped against the human genome (GRCh38/hg38 build) to identify associated mutations. Results Two novel deleterious variants in COL4A3 and COL4A4 loci on chromosome 2 were identified. The variants were detected in the probands with mutant alleles in the homozygous state, a premature stop codon at position 481 of COL4A3 protein and a frameshift mutation leading to a stop codon at position 786 of COL4A4 protein. For both Alport cases the putative variants were surrounded by broad Runs of Homozygosity as well as genes associated with other hereditary nephropathies. Genotyping for COL4A3 validated the exome findings. Conclusions Two novel variants were identified in two unrelated families from northeast of Brazil. The two deleterious variants identified are located on ROH´s locus with all variants in a homozygous state. |
| format | Article |
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| institution | DOAJ |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-04-01 |
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| series | BMC Genomics |
| spelling | doaj-art-3ea72e95114940d78f3093810b29b1e12025-08-20T03:08:05ZengBMCBMC Genomics1471-21642025-04-0126111010.1186/s12864-025-11466-4Whole exome sequencing shows novel COL4A3 and COL4A4 variants as causes of Alport syndrome in Rio Grande do Norte, BrazilWashington Candeia de Araújo0Raul Maia Falcão1Raquel Araujo Costa Uchoa2Carlos Alexandre Garcia3Arthur Quintiliano Bezerra da Silva4Kesia Larissa Medeiros Quirino5Francisco Paulo Freire-Neto6Genilson Pereira Gurgel7Paulo Ricardo Porfirio Nascimento8Leonardo Capistrano Ferreira9Priya Duggal10Jorge Estefano S. de Souza11Selma M. B. Jeronimo12Institute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do NorteBiome, Bioinformatics Multidisciplinary Environment, Federal University of Rio Grande do NorteInstitute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do NorteInstitute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do NorteDivision of Nephrology, Department of Medicine, Federal University of Rio Grande do NorteInstitute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do NorteInstitute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do NorteDepartment of Biochemistry, Biosciences Center, Federal University of Rio Grande do NorteInstitute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do NorteInstitute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do NorteDepartment of Epidemiology, Bloomberg School of Public Health, Johns Hopkins UniversityBiome, Bioinformatics Multidisciplinary Environment, Federal University of Rio Grande do NorteInstitute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do NorteAbstract Background Alport syndrome is a progressive and hereditary nephropathy characterized by hematuria and proteinuria as well as extra renal manifestations as hearing loss and eye abnormalities. The disease can be expressed as autosomal recessive or autosomal dominant at COL4A3 and COL4A4 loci, respectively, or X-linked at the COL4A5 locus. This study investigated two unrelated families with nephropathy from Brazil with the aim to identify the mutations involved with the disease. Methods Whole Exome Sequencing was performed for 4 people from each pedigree (case, parents and a sibling). DNA sequences were mapped against the human genome (GRCh38/hg38 build) to identify associated mutations. Results Two novel deleterious variants in COL4A3 and COL4A4 loci on chromosome 2 were identified. The variants were detected in the probands with mutant alleles in the homozygous state, a premature stop codon at position 481 of COL4A3 protein and a frameshift mutation leading to a stop codon at position 786 of COL4A4 protein. For both Alport cases the putative variants were surrounded by broad Runs of Homozygosity as well as genes associated with other hereditary nephropathies. Genotyping for COL4A3 validated the exome findings. Conclusions Two novel variants were identified in two unrelated families from northeast of Brazil. The two deleterious variants identified are located on ROH´s locus with all variants in a homozygous state.https://doi.org/10.1186/s12864-025-11466-4Alport syndromeCollagenExome |
| spellingShingle | Washington Candeia de Araújo Raul Maia Falcão Raquel Araujo Costa Uchoa Carlos Alexandre Garcia Arthur Quintiliano Bezerra da Silva Kesia Larissa Medeiros Quirino Francisco Paulo Freire-Neto Genilson Pereira Gurgel Paulo Ricardo Porfirio Nascimento Leonardo Capistrano Ferreira Priya Duggal Jorge Estefano S. de Souza Selma M. B. Jeronimo Whole exome sequencing shows novel COL4A3 and COL4A4 variants as causes of Alport syndrome in Rio Grande do Norte, Brazil BMC Genomics Alport syndrome Collagen Exome |
| title | Whole exome sequencing shows novel COL4A3 and COL4A4 variants as causes of Alport syndrome in Rio Grande do Norte, Brazil |
| title_full | Whole exome sequencing shows novel COL4A3 and COL4A4 variants as causes of Alport syndrome in Rio Grande do Norte, Brazil |
| title_fullStr | Whole exome sequencing shows novel COL4A3 and COL4A4 variants as causes of Alport syndrome in Rio Grande do Norte, Brazil |
| title_full_unstemmed | Whole exome sequencing shows novel COL4A3 and COL4A4 variants as causes of Alport syndrome in Rio Grande do Norte, Brazil |
| title_short | Whole exome sequencing shows novel COL4A3 and COL4A4 variants as causes of Alport syndrome in Rio Grande do Norte, Brazil |
| title_sort | whole exome sequencing shows novel col4a3 and col4a4 variants as causes of alport syndrome in rio grande do norte brazil |
| topic | Alport syndrome Collagen Exome |
| url | https://doi.org/10.1186/s12864-025-11466-4 |
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